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LY/T 3108-2019: Preparation procedure of rattan specimens
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Preparation procedure of rattan specimens
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LY/T 3108-2019
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Basic data | Standard ID | LY/T 3108-2019 (LY/T3108-2019) | | Description (Translated English) | Preparation procedure of rattan specimens | | Sector / Industry | Forestry Industry Standard (Recommended) | | Classification of Chinese Standard | B60 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 10,195 | | Date of Issue | 2019-10-23 | | Date of Implementation | 2020-04-01 | | Issuing agency(ies) | State Forestry and Grassland Administration | LY/T 3108-2019: Preparation procedure of rattan specimens---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Palm vine plant specimen making rules)
ICS 65.020.01
B 60
LY
People's Republic of China Forestry Industry Standard
Palm vine plant specimen making rules
Preparation procedure of rattan specimens
2019-10-23 released
2020-04-01 implementation
Published by the National Forestry and Grassland Administration
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed and managed by the National Bamboo and Rattan Standardization Technical Committee (SAC/TC263).
Drafting organizations for this standard. Institute of Tropical Forestry, Chinese Academy of Forestry, South China Botanical Garden, Chinese Academy of Sciences, Southwest Forestry University
learn.
The main drafters of this standard. Li Rongsheng, Yin Guangtian, Guo Lixiu, Wang Kuanglin, Yang Jinchang, and Zou Wentao.
Palm vine plant specimen making rules
1 Scope
This standard specifies the technical requirements for the collection, processing, binding and preservation of palm vine plant specimens.
This standard applies to the preparation of palm vine plant wax leaf specimens.
2 Normative references
The following documents are essential for the application of this document. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
GB/T 26914-2011 palm vine terminology
LY/T 2052 Palm vine and rattan defects
3 terms and definitions
The terms and definitions defined in GB/T 26914-2011 and LY/T 2052 and the following terms apply to this document.
3.1
Pinna, leaflet
Lobules with pinnatifid or entire leaves.
Note. Modified GB/T 26914-2011, terms and definitions 3.12.
3.2
Leaf rachis
A strip-shaped organ on the leaf starting from the position where the first feathers were born to the end of the last one.
3.3
Leaf sheath
The base of the leaf expands into a tubular and cane-like organ.
[GB/T 26914-2011, terms and definitions 3.8]
3.4
Axe cirrus
A whiplike organ extending from the apex of the leaf axis with spines but without feathers.
[GB/T 26914-2011, terms and definitions 3.13]
3.5
Sheath whip flagellum
Whip-shaped organs born with spines but without feathers on leaf sheaths.
[GB/T 26914-2011, terms and definitions 3.14]
3.6
Knee
The structure of the petiole and leaf sheath is a sac-like protrusion.
[GB/T 26914-2011, terms and definitions 3.10]
3.7
Stipule sheath
A protrusion extending from where the sheath and petiole meet.
Note. Modified GB/T 26914-2011, term and definition 3.9.
4 Acquisition
4.1 Materials and tools
Branch shears, telescopic branch shears, high branch shears, pliers, tweezers, specimen holders, collection bags (boxes), straw paper, corrugated paper, positioners, tape measure
Tag, pen, paper bag, plastic bag, silicone, record book, etc.
4.2 Object
Stems, leaves, inflorescences, and inflorescences that are fully developed, highly representative, and disease-free. Vine species with climbing organs should also collect sheath whip or axe whip.
4.3 Process
Firstly, the petiole is disconnected in the middle of the petiole, and the pinion and axillary whip (with axe vine species) of the distal end of the petiole and its shaft are collected;
Sheath whip vine species), inflorescence or inflorescence base disconnected from leaf sheath, collecting sheath whip, inflorescence or inflorescence; finally the main stem is cut
Non-endangered vine species), collecting leaf sheaths and canes.
4.4 Requirements
4.4.1 Petiole
Petioles ≤ 30 cm in length should be collected intact. For petioles > 30cm, representative petiole segments must be collected. Petioles from 30 cm to 60 cm
It should be collected at the base and top. Petioles > 60 cm are generally collected at the base, middle and top, and the length of each segment is ≤30 cm.
4.4.2 Feather
Feathers should be collected together with leaf axis. Leaf blades with leaf axis length ≤ 30 cm should be collected intact, and leaves with leaf axis length > 30 cm should be selected
Feather segments with representative morphological characteristics are generally collected at the base, middle, and top, and each segment is ≤30 cm in length.
Collected feathers with a feather length of < 15cm can be retained. Only feathers with a feather length of> 30 cm should be retained on one side, and all other feathers on the other side should be retained.
Cut off and keep only the base of the feathers, not exceeding 1 cm in length.
4.4.3 Axle Whip
The shaft whip with a length of ≤100 cm should be collected completely and folded. Axial whip > 100 cm should be selected from representative morphological features.
Generally, it should be collected at the base, middle and top.
4.4.4 Inflorescence
Collect all well-developed inflorescences or parts that have typical classification characteristics. At the same time, collect some flowers and place them in paper or plastic bags.
Add silica gel to dry.
4.4.5 Fruit order
Collect all well-developed fruit sequences or parts that have typical classification characteristics. At the same time, collect some fruits and place them in paper or plastic bags.
Dry into silica gel.
4.4.6 Sheath Whip
The shaft whip with a length of ≤100 cm should be collected completely and folded. Axial whip > 100 cm should be selected from representative morphological features.
Generally, it should be collected at the base, middle and top.
4.4.7 Leaf sheath
The leaf sheath should be collected with a stipule sheath and a small petiole. The exposed part of the leaf sheath ≤ 30 cm should be collected completely;
The leaf sheath of cm can only collect the part 30cm downward from the petiole connection, or the 15cm part of the upper and lower leaf sheaths as the center.
4.4.8 Cane
Canes with obvious canes and not endangered should be collected with canes; canes should be collected with knots; vines with internode length ≤ 30 cm can be collected intact
1 internode with vines with an internode length of > 30 cm. Collect the upper and lower 15 cm sections centered on the node; the collected samples should be free of LY/T 2052.
Defects specified.
4.5 Record
See Appendix A.
4.6 Numbering
The number of the collected plant material is the same as the same plant number, and each part should be marked with the corresponding number. The signing should include the collector,
Basic information such as location, collection number and collection date.
5 Processing
5.1 suppression
Lay the collected specimens one by one on several layers of absorbent paper, and then press the specimen clamps up and down to make them flat as soon as possible.
Pay attention to the following situations when suppressing.
-Try to make the cane, flowers, fruits, and feathers flat, and make the back of some feathers face up to observe the characteristics of the feathers;
-Feathers with a feather length < 15cm can be folded from the base, and feathers with a feather length> 30 cm should be folded in the middle;
-The thorny leaf sheath can be flattened first and then pressed;
-The specimens should be placed with care to ensure that the specimens are balanced, and that the specimens are balanced evenly and not topple over.
5.2 Drying
5.2.1 Paper change drying method
Press the absorbent paper to dry the specimen. Change the absorbent paper once every 1 d to 2 d, and change the paper 10 times.
5.2.2 Thermal drying method
Dry in an oven or hot air blower. The drying temperature should not exceed 45 ° C, and the drying time should not exceed 48 h.
5.3 Disinfection
5.3.1 Cryopreservation
Seal the dried specimen in a plastic bag and put it in a low-temperature refrigerator below -18 ° C for 7 d, or in a -30 ° C refrigerator for 3 d or a -50 ° C refrigerator 1
d.
5.3.2 Ultraviolet disinfection method
Place under UV light for 1 h.
6 Staple
6.1 Materials and tools
Specimen paper, scissors, utility knife, pencil, gel, gloves, needlework, etc.
6.2 Permutations
6.2.1 Integer
The dried specimens were further trimmed and shaped to preserve the useful classification features of each part. If there are too many feathers, you can cut off some feathers
Piece, but leave the base of the feather piece to show where the feather piece was cut.
6.2.2 Placement
Specimen placement should follow the following principles.
-Reserve labels and paper bags;
-The specimen should be selected for the best side to show as many features as possible;
-The feathers need to be displayed on 2 sides, and the flowers should be displayed on 2 sides as much as possible;
--The specimens should be placed in the wrong direction from beginning to end. Large specimens should be placed diagonally. Excessively long specimens can also be folded into "V", "N" or
"W" type placement.
-When the same number of specimens cannot be placed on a single sheet of paper, the specimens can be placed on different sheets of paper separately, and each sheet should be marked with the same collection
And the total number of sets of paper, and add A, B, C, D or -1, -2, -3, -4 to the collection number to indicate the difference.
6.3 Fixed
The placed specimens should be fixed with sutures or adhesive banding. When using adhesive strapping to fix, use a pencil to select a few fixed points on the plant specimen
Remember, use a utility knife to cut a gap of 0.5 cm to 1.0 cm in length, use a thin strip of paper to pass through the gap, and fix the back with glue.
Fragments such as flowers, fruits, and leaves are packed in separate small paper bags and pasted in blank spaces. Lay flat on a dry place until the glue completely fixes the specimen.
6.4 Label
Enter the content of the record into the computer and print it, and affix the printed specimen collection record label to the upper left corner of the paper, see B1 in Appendix B;
The printed specimen identification label is affixed to the bottom right corner, see B2 in Appendix B.
7 Save
7.1 Packaging
The prepared specimens should be packed in cardboard or placed in a specimen box, and the paper clip or box should be labelled. The label contains the Chinese name of the rattan and Latin
Scientific name information.
7.2 Storage conditions
Specimens should be stored in a specimen cabinet, which should be protected from sunlight and other light sources; the temperature of the place where the specimen cabinet is located should be controlled at
20 ℃ ~ 23 ℃, relative humidity is 40% ~ 55%.
Appendix A
(Informative appendix)
Contents of palm vine plant specimen collection records
I. Origin Information
1. Collection location.
2. Longitude.
3. Latitude.
Habitat and ecological information
(1) Geographical environment
Terrain
2. Small terrain.
3. Altitude. m
(II) Vegetation information
1. Vegetation type.
2. associated plant
(1) Arbor.
(2) Shrubs.
(3) Fujimoto.
(4) Herbs.
3. Population information
(1) Quantity.
(2) Common or not.
(3) Survival methods.
(4) Frequency of feature variation.
Third, habits
1. Life type.
2. Branch type.
Third, species biological information
(A) the root system
1. Root system type.
2. Distribution characteristics.
3. Root incision odor.
(B) the stem
1. Total stem number.
2. Maximum stem length.
3. Color.
4. Texture.
5. Color.
6. Wax layer.
7. Diameter. cm
8.Section diameter. cm
6.Inner diameter of section. cm
8. Internode length. cm
(Three) leaves
Leaf sheath.
2. Sheath whip.
3. Knee process.
4. Stipule sheath.
5. Petiole.
6. Leaf axis.
7. Feather.
8. Axle whip.
9. Basal leaf characteristics.
(Four) inflorescence
1. Location.
2. Number of times.
3. Length.
4. Branch inflorescence.
5. Bracts.
6. Pattern.
(Five) flowers
Style
2. Sex of the flower.
3. The smell of flowers.
4. Flower shape.
5. Corolla color. Corolla texture.
6. Calyx color. Calyx texture.
7. Secretions or glands.
8. Behavior.
9. Pollen Media.
(6) Fruits and seeds
1. Odor.
2. Color. Texture.
3. Size. Shape.
4. Scale characteristics. Number of scales.
5. Seed coat color. Seed coat.
6. Color of aril coat.
7. Seed shape. Seed size.
8. Spread.
(7) Native name
1. Native names.
2. Detailed name of the native name.
(Eight) uses
1. Application organ.
2. Application function or purpose.
Appendix B
(Informative appendix)
Palm vine plant specimen collection and identification information label format
Palm vine plant specimens should be affixed with labels in the B1 and B2 format on the bottom and bottom corners, respectively.
B1. Sample collection record label
Collection unit name
Acquisition records
Specimen number.
Collected by. Collection number.
Collection date. year, month, day
Collection location.
Small terrain. Altitude. m
Vegetation Types.
surroundings.
Features. height m; DBH cm
leaf.
flower.
fruit.
Local use.
Section name.
Field identification species name.
Land name (specify language).
PS.
B2. Specimen identification label
Herbarium Room Name
Specimen number.
Middle name.
scientific name.
Collected by. Collection number.
Appraiser. Appraisal time.
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