JJG 646-2006_English: PDF (JJG646-2006)
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Verification Regulation of Locomotive Pipette
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JJG 646-2006
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Standards related to: JJG 646-2006
| Standard ID | JJG 646-2006 (JJG646-2006) | | Description (Translated English) | Verification Regulation of Locomotive Pipette | | Sector / Industry | Metrology & Measurement Industry Standard | | Classification of Chinese Standard | A53 | | Classification of International Standard | 17.120 | | Word Count Estimation | 18,191 | | Date of Issue | 2006-12-08 | | Date of Implementation | 2007-06-08 | | Older Standard (superseded by this standard) | JJG 646-1990 | | Quoted Standard | GB/T 6682 | | Drafting Organization | China Institute of Metrology | | Administrative Organization | National Flow & Volume Capacity Measurement Technical Committee | | Regulation (derived from) | AQSIQ Announcement No. 187 of 2006 | | Issuing agency(ies) | State Administration of Quality Supervision, Inspection and Quarantine | | Summary | This standard applies to the pipette initial verification, testing and use of follow-up inspection. | JJG 646-2006
JJG
National Metrological Verification Regulation
of the People's Republic of China
Locomotive Pipette
移液器
ISSUED ON. DECEMBER 08, 2006
IMPLEMENTED ON. JUNE 08, 2007
Issued by. The State Administration of Quality Supervision, Inspection
and Quarantine
This Regulation was approved by the General Administration of Quality Supervision,
Inspection and Quarantine of the People’s Republic of China on December 8, 2006 and
was implemented on June 8, 2007.
Jurisdiction organization. National Technical Committee of Flow Capacity Measurement
Main drafting organization. National Institute of Metrology
Participating organization. Shanghai Institute of Measurement and Testing Technology
National Technical Committee of Flow Capacity Measurement is responsible for the
interpretation of this Regulation
Main drafter of this Regulation.
Verification Regulation of
Locomotive Pipette
replacing JJG 646-1990
Zhang Long (National Institute of Metrology Science)
Participating drafters.
Tong Lin (National Institute of Metrology Science)
Bao Xuesong (National Institute of Metrology Science)
Zhang Hongya (Shanghai Institute of Measurement and Metrology Technology)
Table of Contents
1 Scope... 5
2 Cited reference ... 5
3 Terms and measurement units ... 5
4 Overview ... 6
5 Metrological properties requirements ... 6
6 General technical requirements ... 8
6.1 Appearance requirements ... 8
6.2 Piston ... 8
6.3 Regulator ... 8
6.4 Aspirating-nozzle ... 8
6.5 Air-tightness ... 8
6 Metrological measuring tool control ... 8
7.1 Verification conditions ... 8
7.2 Verification items ... 9
7.3 Verification method ... 9
7.4 Verification results processing ... 13
7.5 Verification period ... 13
Annex A Schematic diagrams for various locomotive pipettes ... 14
Annex B K(t) value table... 17
Annex C Recording format of locomotive pipette verification ... 18
Verification Regulation of Locomotive Pipette
1 Scope
This Regulation applies to the initial verification, subsequent verification and in-service
inspection of locomotive pipette.
2 Cited reference
This Regulation cites the following document.
GB 6682-1992 Water for analytical laboratory use – Specification and test methods
Pay attention to use the current valid version of the above cited reference in using this
Regulation.
3 Terms and measurement units
3.1 Locomotive pipette
Measuring instrument with a certain scale range that can suck the liquid from container
and transfer it into another container (Filling injector, sample-filling storage, aspirator etc.
collectively referred to as locomotive pipettes).
3.2 Adjustable locomotive pipette
Locomotive pipette whose volume value can be adjusted.
3.3 Quantitative locomotive pipette
Locomotive pipette with single volume value.
3.4 Aspirating-nozzle
The part installed on the lower end of locomotive pipette and used to suck and discharge
liquid.
3.5 Display window
The window on locomotive pipette displaying volume value.
3.6 Suction tube
The connecting member between locomotive pipette and aspirating-nozzle.
3.7 Volume regulator
The rotary knob of digital device used to regulate volume.
3.8 Measurement unit
The measurement unit of locomotive pipette is µL or mL.
4 Overview
Locomotive pipettes are mainly used in environmental protection, medicine, food and
health research department, and other departments to sample liquid or add liquid in
biochemical analysis and assays. It is a piston pipette working with the principle of air
discharge and determining the volume of the locomotive pipette through the moving
distance of piston in piston sleeve.
Locomotive pipette is ex-quantity measurement instrument, which is divided into
quantitative locomotive pipette and adjustable locomotive pipette. Its type is divided into
single-channel type and multichannel type. Its structure consists of display window, volume
adjustable member, piston, piston sleeve, suction tube, aspirating-nozzle and so on (See
Figure A.1-10).
5 Metrological properties requirements
At the standard temperature 20°C, the volume permissible tolerance and measurement
repeatability of the locomotive pipette shall meet the requirements in Table 1.
Table 1 Volume permissible tolerance and measurement repeatability of
locomotive pipettes
Nominal volume /μL Monitoring points /μL
Volume permissible
tolerance ± (%)
Measurement repeatability
≤ (%)
0.1 20.0 10.0
0.5 20.0 10.0
1 12.0 6.0
0.2 20.0 10.0
1 12.0 6.0
2 12.0 6.0
0.5 20.0 10.0
1 12.0 6.0
5 8.0 4.0
1 12.0 6.0
5 8.0 4.0
10 8.0 4.0
6 General technical requirements
6.1 Appearance requirements
6.1.1 The appearance of plastic parts of locomotive parts shall be flat and smooth; there
shall be no obvious sink marks, scrap, cracks, blistering and deformation phenomena on
it; the surface coating of metal parts shall be no fall-off, rust and flaking.
6.1.2 The body of locomotive pipette shall have the following tags. product name,
manufacturer or trademark, nominal capacity (μL or mL), model specifications and
manufacturing number.
6.2 Piston
The piston of locomotive pipette shall be able to move up and down flexibly with clear sub-
block boundaries when being pressed.
6.3 Regulator
The display window of locomotive pipette shall be flexible in rotation with clear and
complete indicating numbers in the action of regulating volume.
6.4 Aspirating-nozzle
6.4.1 Aspirating-nozzle shall be made of polypropylene material.
6.4.2 Aspirating-nozzle shall have no obvious bending phenomenon. The internal wall shall
be clean and smooth; there shall be no residual liquid after draining.
6.4.3 Different specifications of locomotive pipettes shall use corresponding matching
aspirating-nozzle.
6.5 Air-tightness
Under 0.04 MPa, the locomotive pipette shall have no air leakage phenomenon in 5
seconds.
6 Metrological measuring tool control
Metrological measuring tool control includes initial verification, subsequent verification and
in-service inspection.
7.1 Verification conditions
7.1.1 Verification environment
The locomotive pipette shall be verified under the condition at (20±5)°C of room
7.3.3.1 Preparation before verification
The selected aspirating-nozzle shall match with the aspirating-rod. At the lower end of the
aspirating-rod of locomotive pipette, gently turn the aspirating-nozzle to ensue the air-
tightness of locomotive pipette; there shall be no hanging-water phenomena after the
process of several times of aspirating and discharging.
Ensure the fixing installation of each aspirating-nozzle of multichannel locomotive pipette
before verification.
7.3.3.2 Verification steps
(1) Place the measuring glass in electronic balance; press the control rod to make the
electronic balance to reset to zero, after the indication is stable.
(2) Regulate the volume of the locomotive pipette to the verified point.
(3) Hold the locomotive pipette vertically; press the button to the verification point; at
this time, place the aspirating-nozzle in the container that contains distilled water;
keep it 2mm-3mm under the liquid level; slowly release the button; after 1 s ~ 2 s,
remove it out of the liquid; wipe out the liquid at aspirating-nozzle (At this time, do
not touch the liquid outlet, so as to avoid sucking away the liquid in aspirating-
nozzle).
(4) Take the measuring glass out of the electronic balance; the liquid-discharge mouth
of aspirating-nozzle is leaned to the inner wall of measuring glass, and is formed
45° with it; slowly press the button to the first stop point; wait for 1 s ~ 2 s; fully
press down the button; then remove the aspirating-nozzle along the inner wall of
measuring glass.
(5) Place the measuring glass on balance’s pan; recode the value displayed by the
balance at this time; meantime, measure and recode the temperature of the
distilled water in the container.
(6) Repeat item (1) to item (5) for six times; the error of each measurement must not
exceed the provisions in Table 1.
7.3.4 Data processing
7.3.4.1 Calculation ...
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