GB 5009.8-2023 PDF English
US$335.00 · In stock · Download in 9 secondsGB 5009.8-2023: National food safety standard - Determination of fructose, glucose, sucrose, maltose and lactose in foods Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedureStatus: Valid GB 5009.8: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| GB 5009.8-2023 | English | 335 |
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National food safety standard - Determination of fructose, glucose, sucrose, maltose and lactose in foods
| Valid |
| GB 5009.8-2016 | English | 85 |
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Determination of saccharose in foods
| Obsolete |
| GB/T 5009.8-2008 | English | 319 |
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3 days
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Determination of saccharose in foods
| Obsolete |
| GB/T 5009.8-2003 | English | 90 |
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Determination of saccharose in foods
| Obsolete |
| GB/T 5009.8-1985 | English | 199 |
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2 days
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Method for determination of saccharose in foods
| Obsolete |
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GB 5009.8-2023: National food safety standard - Determination of fructose, glucose, sucrose, maltose and lactose in foods
---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB5009.8-2023
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
National food safety standard - Determination of fructose,
glucose, sucrose, maltose and lactose in foods
Issued on. SEPTEMBER 06, 2023
Implemented on. MARCH 06, 2024
Issued by. National Health Commission of the People’s Republic of China;
State Administration for Market Regulation.
Table of Contents
Foreword... 3
1 Scope... 4
2 Principle... 4
3 Reagents and materials... 4
4 Instruments and apparatuses... 6
5 Analysis steps... 6
6 Expression of analysis results... 8
7 Precision... 9
8 Others... 9
9 Principle... 9
10 Reagents and materials... 9
11 Instruments and apparatuses... 11
12 Test steps... 11
13 Expression of analysis results... 14
14 Precision... 14
15 Others... 14
16 Principle... 15
17 Reagents and solutions... 15
18 Instruments and apparatuses... 16
19 Analysis steps... 16
20 Expression of analysis results... 19
21 Precision... 21
22 Principle... 21
23 Reagents and materials... 21
24 Instruments and apparatuses... 22
25 Analysis steps... 22
26 Expression of analysis results... 24
27 Precision... 25
1 Scope
This Standard specifies the methods for the determination of fructose, glucose, sucrose,
maltose and lactose in foods.
Method 1 - High performance liquid chromatography applies to the determination of
fructose, glucose, sucrose, maltose and lactose in grains and grain products, milk and
dairy products, fruits, vegetables and fruit and vegetable products, sweeteners, candies,
beverages and infant foods.
Method 2 - Ion chromatography applies to the determination of fructose, glucose,
sucrose, maltose, and lactose in foods.
Method 3 - Acid hydrolysis-Rhein-Eynon method applies to the determination of
sucrose in foods.
Method 4 - Rhine-Enon method applies to the determination of lactose in infant foods
and dairy products.
Method 1 - High performance liquid chromatography
2 Principle
The fructose, glucose, sucrose, maltose and lactose in the sample are extracted, and then
separated by high performance liquid chromatography column, detected by a
differential refractive index detector or an evaporative light scattering detector, and
quantified by the external standard method.
3 Reagents and materials
Unless otherwise specified, all the reagents in this method are analytical reagents, and
the water is grade-1 water specified by GB/T 6682.
3.1 Reagents
3.2 Preparation of reagents
3.3 Standards
3.4 Preparation of standard solutions
3.5 Materials
3.5.2 Syringe.
4 Instruments and apparatuses
4.1 High performance liquid chromatograph. equipped with a differential refractive
index detector or an evaporative light scattering detector.
4.2 Analytical balance. the sensitivity is 1 mg and 10 mg.
4.3 Vortex mixer.
4.4 Centrifuge. speed ≥ 4 000 r/min.
4.5 Ultrasonic cleaner.
4.6 Sample crushing equipment. high-speed crusher.
4.7 Constant-temperature drying oven.
4.8 Constant-temperature water bath device.
5 Analysis steps
5.1 Sample pretreatment
5.1.2 Sample extraction
5.1.3 Purification
For the above sample extract solution, use a filter paper to filter (discard the primary
filtrate) or centrifuge to obtain the supernatant; then, use a 0.45 μm water-based
membrane syringe to filter into a sample bottle for analysis by a high-performance
liquid chromatograph.
5.2 Apparatus reference conditions
Apparatus reference conditions are as below.
90 °C; nitrogen flow rate 2.5 L/min.
5.3 Preparation of the standard curve
Inject the mixed standard working solution into the high-performance liquid
chromatograph in order from low to high concentration, and measure the corresponding
peak areas or peak heights of fructose, glucose, sucrose, maltose and lactose. For the
differential refractive index detector, use the concentration of the standard working fluid
as the abscissa and the peak area or peak height as the ordinate to draw a standard curve;
5.4 Determination of sample solution
Inject the sample solution into the high-performance liquid chromatograph;
qualitatively record the peak area or peak height of the target substance based on the
retention time; obtain the concentrations of fructose, glucose, sucrose, maltose and
lactose in the sample solution based on the standard curve.
5.5 Blank test
Except that no sample is added, proceed according to the above steps.
6 Expression of analysis results
Calculate the contents of fructose, glucose, sucrose, maltose and lactose in the sample
according to Formula (1).
7 Precision
The absolute difference of 2 independent test results obtained under repeatability cannot
exceed 10% of the arithmetic mean value.
8 Others
When the weighing sample is 10 g and the fixed volume is 100 mL, the method
detection limits for fructose, glucose, sucrose, maltose and lactose are all 0.2 g/100 g,
and the quantification limits are all 0.5 g/100 g.
9 Principle
After extraction and purification, the fructose, glucose, sucrose, maltose and lactose in
the sample are separated by an ion chromatography column, detected by a pulse ampere
detector, and quantified by the external standard method.
10 Reagents and materials
Unless otherwise specified, all the reagents in this method are analytical reagents, and
the water is grade-1 water specified by GB/T 6682.
10.1 Reagents
10.1.1 Sodium hydroxide (NaOH). chromatographic pure.
10.1.2 Glacial acetic acid (CH3COOH).
10.2 Preparation of reagents
10.3 Standards
10.3.1 Fructose (C6H12O6, CAS number. 57-48-7). purity ≥99%, or standard material
certified by the country and awarded a standard material certificate.
10.3.2 Glucose (C6H12O6, CAS number. 50-99-7). purity ≥99%, or standard material
certified by the country and awarded a standard material certificate.
10.4 Preparation of standard solution
10.5 Materials
10.5.1 0.45 μm water-based membrane syringe filter (except cellulose membrane).
10.5.2 Purification column. C18 solid-phase extraction cartridge (1.0 mL) or one with
equivalent performance.
10.5.3 Syringe.
11 Instruments and apparatuses
11.1 Ion chromatograph. equipped with pulse ampere detector.
11.2 Sample crushing equipment. high-speed crusher.
11.3 Ultrasonic cleaner.
11.4 Analytical balance. sensitivity 1 mg.
11.5 Vortex mixer.
11.6 Centrifuge. speed ≥ 4 000 r/min.
11.7 Constant-temperature drying oven.
11.8 Constant-temperature water bath device.
12 Test steps
12.1 Sample pretreatment
12.1.1 Sample preparation
Take an appropriate amount of representative sample; for drinks and other liquid
homogeneous samples, shake directly; for non-uniform samples, homogenize or crush
evenly; for frozen drinks, melt at room temperature, stir thoroughly and, if necessary,
heat and stir in a water batch at 30 °C ~ 40 °C; for sauces, grind or homogeneously mix;
for chocolate, heat and melt in a water bath at 50 °C ~ 60 °C, and stir thoroughly while
it is hot.
12.1.2 Sample extraction
12.1.3 Sample purification
The sample extraction solution can be diluted by an appropriate multiple according to
the target sugar content in the sample. When detecting lactose in infant formula milk
powder, dilute it 1 000 times and then purify it; when detecting high sugar content in
honey and candy, dilute it 500 times and then purify it.
After filtering or centrifuging the above sample extraction solution or dilution solution
to obtain the supernatant, pass it through a 0.45 μm aqueous membrane syringe filter
and a C18 solid phase extraction cartridge (1.0 mL) in sequence; discard the first 3 mL;
collect subsequent eluates for testing.
12.2 Apparatus reference conditions
Apparatus reference conditions are as below.
12.3 Drawing of standard working curve
Inject the mixed standard working solution into the ion chromatograph in order from
low to high concentration, and measure the corresponding peak areas of fructose,
glucose, sucrose, maltose and lactose. Taking the concentrations of fructose, glucose,
sucrose, maltose and lactose in the standard working solution as the abscissa and the
peak area as the ordinate, draw the standard curves, respectively. See Appendix B for
the ion chromatograms of standard solutions of fructose, glucose, sucrose, maltose and
lactose.
12.4 Determination of sample solution
Inject the sample solution into the ion chromatograph, identify it based on the retention
time, record the peak area, and obtain the concentrations of fructose, glucose, sucrose,
maltose and lactose in the sample solution based on the standard curve.
12.5 Blank test
Except that no sample is added, proceed according to the above steps.
13 Expression of analysis results
Calculate the contents of fructose, glucose, sucrose, maltose and lactose in the sample
according to Formula (2).
14 Precision
The absolute difference of 2 independent test results obtained under repeatability cannot
exceed 10% of the arithmetic mean value.
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
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