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GB/T 4789.10-2008 (GBT 4789.10-2008)

GB/T 4789.10-2008_English: PDF (GBT4789.10-2008)
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GB/T 4789.10-2008English599 Add to Cart Days<=4 Microbiological examination of food hygiene -- Detection of staphylococcus aureus GB/T 4789.10-2008 Obsolete GB/T 4789.10-2008

Standard ID GB/T 4789.10-2008 (GB/T4789.10-2008)
Description (Translated English) Microbiological examination of food hygiene. Detection of staphylococcus aureus
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard C53
Classification of International Standard 07.100.30
Word Count Estimation 15,136
Date of Issue 2008-11-21
Date of Implementation 2009-03-01
Older Standard (superseded by this standard) GB/T 4789.10-2003
Adopted Standard AOAC 987.09, NEQ; ISO 6888-1-1999, NEQ
Drafting Organization China Disease Prevention and Control Center of Nutrition and Food Safety
Administrative Organization Ministry of Health
Regulation (derived from) National Standard Approval Announcement 2008 No.22 (Total No.135); Health-Communication (2010) 7; National Standard Approval Announcement 2010 No.5 (Total No.160)
Proposing organization People's Republic of China Ministry of Health
Issuing agency(ies) Ministry of Health of People's Republic of China; Standardization Administration of China
Summary This standard specifies test methods Staphylococcus aureus in food.

GB/T 4789.10-2008
Microbiological examination of food hygiene. Detection of staphylococcus aureus
ICS 07.100.30
National Standards of People's Republic of China
Replacing GB/T 4789.10-2003
Microbiological examination of food hygiene
Staphylococcus aureus test
Posted 2008-11-21
2009-03-01 implementation
People's Republic of China Ministry of Health
Standardization Administration of China released
This standard corresponds to international analysts Society (AOACINTERNATIONAL) AOAC987.09 "food in Staphylococcus aureus
mostprobablenumbermethodforisolationandenumeration) and the International Organization for Standardization ISO 6888-1.1999 "food
Products and animal feed coagulase positive Staphylococcus aureus and other staphylococci counting method "(Microbiology
um). The Standard and AOACOfficialMethod987.09, ISO 6888-1.1999 is not equivalent degree of consistency.
This standard replaces GB/T 4789.10-2003 "Microbiological examination of food hygiene inspection of Staphylococcus aureus."
This standard compared with GB/T 4789.10-2003 The main changes are as follows.
--- Standardized sample preparation process;
--- The original standard coagulase section has been modified.
This standard Annex A, Annex B normative appendix.
This standard is proposed and administered by the People's Republic of China Ministry of Health.
This standard by the People's Republic of China Ministry of Health is responsible for interpretation.
This standard is drafted by. China Center for Disease Control Nutrition and Food Safety.
Involved in the drafting of this standard. Shanghai Disease Prevention and Control Center, People's Republic of China Liaoning Exit Inspection and Quarantine, the Chinese people
Inner Mongolia, People's Republic of Exit Inspection and Quarantine, Jiangsu Province Center for Disease Control and Prevention.
The main drafters of this standard. Xiumei, Chen Min, Liu Hong, Liu high school, Lu Xingan, Yuan Baojun, Tian Jing.
This standard replaces the standards previously issued as follows.
--- GB 4789.10-1984, GB/T 4789.10-1994, GB/T 4789.10-2003.
Microbiological examination of food hygiene
Staphylococcus aureus test
1 Scope
This standard specifies the test methods in food Staphylococcus aureus.
This standard applies to all types of food and food poisoning Staphylococcus aureus samples tested.
2 Equipment and Materials
In addition to the microbiological laboratory conventional sterilization and cultivation equipment, other equipment and materials as follows.
2.1 incubator. 36 ℃ ± 1 ℃.
2.2 Refrigerator. 2 ℃ ~ 5 ℃.
2.3 constant temperature water bath. 37 ℃ ± 1 ℃.
2.4 Balance. a sense of the amount of 0.1g.
2.5 homogenizer.
2.6 oscillator.
2.7 sterile pipette. 1mL (with 0.01mL scale), 10mL (with 0.1mL scale) or micro pipettes and tips.
2.8 sterile conical flask. capacity 100mL, 500mL.
2.9 sterile Petri dish. diameter of 90mm.
2.10 syringe. 0.5mL.
2.11 pH meter or pH colorimetric tubes or precision pH test paper.
3 media and reagents
3.1 10% sodium chloride trypticase soy broth. see Section A. Chapter 1.
3.2 7.5% sodium chloride broth. see Section A. 2.
3.3 blood agar plates. see Section A. 3.
3.4 Baird-Parker agar plates. see Section A. 4.
3.5 brain heart infusion (BHI) broth. See Section A. 5.
3.6 Rabbit plasma. see Section A. 6.
3.7 phosphate buffer. see Section A. 7.
3.8 nutrient agar slant. see Section A. 8.
3.9 Gram stain solution. See Section A. 9.
Sterile saline 3.10. Weigh 8.5g of sodium chloride dissolved in 1000mL of distilled water, 121 ℃ autoclave 15min.
3.11 1mol/L sodium hydroxide (NaOH). Weigh 40g of sodium hydroxide (NaOH) was dissolved in 1000mL of distilled water.
3.12 1mol/L hydrochloric acid (HCl). 37% concentrated hydrochloric acid 90mL, add distilled water to 1000mL.
4 inspection procedures
Test procedure shown in Figure 1.