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GB/T 42821-2023: Diagnostic methods for bonamiosis of molluscs
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| GB/T 42821-2023 | English | 349 |
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Diagnostic methods for bonamiosis of molluscs
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GB/T 42821-2023
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Basic data | Standard ID | GB/T 42821-2023 (GB/T42821-2023) | | Description (Translated English) | Diagnostic methods for bonamiosis of molluscs | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 65.020.30 | | Word Count Estimation | 18,116 | | Date of Issue | 2023-08-06 | | Date of Implementation | 2024-03-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 42821-2023: Diagnostic methods for bonamiosis of molluscs---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 65.020.30
CCSB41
National Standards of People's Republic of China
Diagnostic Methods for Nanoplasia in Shellfish Bags
Published on 2023-08-06
2024-03-01 Implementation
State Administration for Market Regulation
Released by the National Standardization Administration Committee
Table of contents
Preface III
1 Scope 1
2 Normative reference documents 1
3 Terms and Definitions 1
4 Abbreviations 1
5 Reagents and Materials 1
6 Equipment and equipment2
7 Clinical symptoms 3
8 sample 3
9 Organize print inspection 3
10 Histopathological examination 3
11 PCR Test 4
12 Fluorescence PCR detection 5
13 Comprehensive judgment 6
Appendix A (normative) Preparation of tissue prints, histopathological examination and DNA extraction related solutions 7
Appendix B (informative) Shellfish bag nanodisease10
Appendix C (Informative) Target amplification sequence and the locations of primers and probes 13
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some content in this document may be subject to patents. The publisher of this document assumes no responsibility for identifying patents.
This document is proposed by the Ministry of Agriculture and Rural Affairs of the People's Republic of China.
This document is under the jurisdiction of the National Fisheries Standardization Technical Committee (SAC/TC156).
This document was drafted by. Chinese Academy of Inspection and Quarantine, Chinese Academy of Fisheries Sciences Yellow Sea Fisheries Research Institute, National Fisheries Technology
Promotion Headquarters, Guangzhou Customs.
The main drafters of this document. Wu Shaoqiang, Wang Caixia, Bai Changming, Li Qing, Deng Yan, Feng Chunyan, Wang Chongming, Yin Hongda, Yuan Xiangfen, Xu Xiaolin,
Bai Jianshan.
Diagnostic Methods for Nanoplasia in Shellfish Bags
1 Scope
This document gives the abbreviations, reagents and materials, instruments and equipment for the diagnosis of shellfish bonamiosis, and describes the clinical
Symptoms, sampling, tissue print examination, histopathological examination, PCR detection, fluorescent PCR detection and comprehensive judgment methods.
This document applies to shellfish encrustation caused by Bonamiaostreae and Bonamiaexitiosa
Epidemiological investigation, diagnosis, quarantine and surveillance of nanodiseases.
2 Normative reference documents
The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, the dated quotations
For undated referenced documents, only the version corresponding to that date applies to this document; for undated referenced documents, the latest version (including all amendments) applies to
this document.
GB/T 6682 Specifications and test methods for water used in analytical laboratories
GB/T 18088 Entry and Exit Animal Quarantine Sampling
GB/T 19495.2 Technical requirements for genetically modified product testing laboratories
3 Terms and Definitions
There are no terms or definitions to be defined in this document.
4 Abbreviations
The following abbreviations apply to this document.
bp. base pair (basepair)
Ct. amplification cycle number (cyclethreshold)
FAM. carboxyfluorescein
Taq. Thermusaquaticus
TE. Tris hydrochloric acid and EDTA buffer solution (EDTATris·HCl)
5 Reagents and materials
All chemical reagents in this document, unless otherwise specified, use only analytically pure reagents.
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