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GB/T 42699.2-2023: Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 2: Peptide detection using MALDI-TOF-MS
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Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 2: Peptide detection using MALDI-TOF-MS
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GB/T 42699.2-2023
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Basic data | Standard ID | GB/T 42699.2-2023 (GB/T42699.2-2023) | | Description (Translated English) | Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 2: Peptide detection using MALDI-TOF-MS | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | W04 | | Classification of International Standard | 59.080.01 | | Word Count Estimation | 22,283 | | Date of Issue | 2023-08-06 | | Date of Implementation | 2024-03-01 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 42699.2-2023: Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 2: Peptide detection using MALDI-TOF-MS
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ICS 59.080.01
CCS W04
National Standards of People's Republic of China
Textile proteome characterization of certain animal hair fibers
and Quantitative Analysis Part 2.Reduced Protein Peptides
Analytical matrix-assisted laser desorption ionization
Time-of-flight mass spectrometry (MALDI-TOF-MS) method
(ISO 20418-2.2018,MOD)
Published on 2023-08-06
2024-03-01 Implementation
State Administration for Market Regulation
Released by the National Standardization Administration Committee
Table of contents
PrefaceⅠ
Introduction II
1 Scope 1
2 Normative reference documents 1
3 Terms and Definitions 1
4 Abbreviations 2
5 Principle 2
6 reagent 2
7 Instruments and Utensils 4
8 Test step 4
9 Precision 7
10 Interlaboratory Test 7
11 Test report 7
Appendix A (informative) Example 8 of SDS-PAGE gel image of extracted protein
Appendix B (Informative) Animal species-specific mass spectrum peak 9 analyzed by MALDI-TOF-MS
Appendix C (Informative) Species-specific monoisotopic peaks in MALDI-TOF-MS analysis10
Appendix D (informative) Standard curve for cashmere-sheep wool mixture 11
Appendix E (informative) Standard curve of cashmere-yak blend 12
Appendix F (Informative) Repeatability and Reproducibility 13
Appendix G (informative) MALDI-TOF-MS blind sample detection and analysis 15
Appendix H (Informative) Interlaboratory Validation 16
Reference 19
Foreword
This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
This document is Part 2 of GB/T 42699 "Qualitative and Quantitative Analysis of Proteome of Certain Animal Hair Fibers in Textiles".
GB/T 42699 has released the following parts.
---Part 1.Reduced protein peptide analysis liquid chromatography-mass spectrometry (LC-ESI-MS) method;
---Part 2.Reduced protein peptide analysis matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) method.
This document is modified using ISO 20418-2.2018 "Qualitative and quantitative analysis of proteome of certain animal hair fibers in textiles - Part 2"
Points. Reduced protein peptide analysis matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) method.
The technical differences between this document and ISO 20418-2.2018 and their reasons are as follows.
---Added warning;
---In Chapter 1, ISO 1833 (all parts) is replaced by our country's document GB/T 2910 (all parts), FZ/T 01057.3 generation
Replaced ISO /T R11827, GB/T 40905 (all parts) replaced ISO 17551 (all parts) to adapt to our country's technology
technical conditions;
---In Chapter 6, the regulations on reagents are made in line with the actual situation in our country;
---In 6.1, ISO 3696 is replaced by my country's document GB/T 6682 to adapt to my country's technical conditions;
---In 6.4, the mass of sodium dihydrogen phosphate is corrected to 24g;
---In 6.14, the CAS number of the substance is added;
---In Chapter 7, 7.9 "centrifuge tube", 7.10 "96-well microplate", 7.11 "pH meter" and 7.12 "centrifuge" are added;
---In 8.3.4, the recycling strip size is increased to make it more operable;
---In 8.5.1, change the reflection mode to reflection mode or linear mode.
The following editorial changes have been made to this document.
---In 3.1, the definition of animal hair fiber is adjusted to be consistent with the definition in ISO 20418-1.2018.
Please note that some content in this document may be subject to patents. The publisher of this document assumes no responsibility for identifying patents.
This document is proposed by the China Textile and Apparel Industry Federation.
This document is under the jurisdiction of the National Textile Standardization Technical Committee (SAC/TC209).
This document was drafted by. Shanghai Customs Industrial Products and Raw Materials Testing Technology Center, China Textile Standard Inspection and Certification Co., Ltd., Anhui Zheng
Lin Textile Co., Ltd., Wujiang Meijin Weaving Co., Ltd., Zhejiang Yilei Wool Textile Co., Ltd., Changxing Kingfa Textile Co., Ltd., Jinjiang
Guangzhilin Knitting Co., Ltd., Shanghai Aili Textile Technology Inspection Co., Ltd., Dongguan Weiside Technology Development Co., Ltd., Hunan Keli
Jia Textile Co., Ltd., Yiwu Niqing Household Products Co., Ltd.
The main drafters of this document. Fei Jing, Chen Xiao, Fei Yafei, Wei Mengyuan, Si Ying, Gao Linmei, Yao Ling, Wang Ya, Zhang Chunhua, Shen Yilei, Jin Fei,
Zhang Wenfu, Xu Shanwen, Zhang Zhihuan.
Introduction
For the qualitative and quantitative analysis test methods of certain animal hair fiber proteomes in textiles, for the convenience of use, according to different test principles
The principles and methods are divided into multiple parts. GB/T 42699 "Qualitative and Quantitative Analysis of Proteome of Certain Animal Hair Fibers in Textiles" is planned to be composed of the following two parts.
Composed of parts.
---Part 1.Reduced protein peptide analysis liquid chromatography-mass spectrometry (LC-ESI-MS) method. The purpose is to establish the use of liquid phase color
Spectrometry-mass spectrometry (LC-ESI-MS) is a qualitative and quantitative method for determining the components of animal hair fiber mixtures in textiles.
---Part 2.Reduced protein peptide analysis matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) method.
The purpose is to establish the use of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to determine the dynamic properties of textiles.
Qualitative and quantitative methods for the components of wool fiber mixtures.
Textile proteome characterization of certain animal hair fibers
and Quantitative Analysis Part 2.Reduced Protein Peptides
Analytical matrix-assisted laser desorption ionization
Time-of-flight mass spectrometry (MALDI-TOF-MS) method
Warning---Personnel using this document should have practical experience in regular laboratory work. This document does not address all possible security issues
question. Users are responsible for taking appropriate safety and health measures and ensuring compliance with the conditions stipulated in relevant national regulations.
1 Scope
This document specifies the use of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for the determination of animal hair in textiles.
Qualitative and quantitative methods for fiber mixture components.
Non-animal hair fiber components can be tested with reference to GB/T 2910 (all parts), and then the results are combined to determine the overall fiber
content.
This method refers to FZ/T 01057.3 and uses an optical microscope to observe the appearance of mixed fibers for preliminary identification. This method is not suitable
Used when animal fibers of the same species are present (e.g. mixtures of cashmere and mohair), such samples can be analyzed by microscopic examination
Quantitative (such as the analytical methods described in GB/T 40905.1 and GB/T 40905.2).
2 Normative reference documents
The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, the dated quotations
For undated referenced documents, only the version corresponding to that date applies to this document; for undated referenced documents, the latest version (including all amendments) applies to
this document.
GB/T 6682 Analytical laboratory water specifications and test methods (GB/T 6682-2008, ISO 3696.1987, MOD)
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
animal hair fiber animalhairfibre
Animal hair follicles grow from fibers with a multicellular structure composed of keratin.
Examples. cashmere, sheep wool, yak wool, etc.
3.2
protein protein
Amino acid polymers that play important roles in living things.
3.3
polypeptide
A small fragment of protein (3.2) formed by dehydration and condensation of less than 50 amino acids.
3.4
buffer solution
A solution that maintains the pH of the reaction solution within an appropriate range.
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