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GB/T 40192-2021: Detection and identification of Colletotrichum Corda using real-time PCR
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Detection and identification of Colletotrichum Corda using real-time PCR
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GB/T 40192-2021
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Basic data | Standard ID | GB/T 40192-2021 (GB/T40192-2021) | | Description (Translated English) | Detection and identification of Colletotrichum Corda using real-time PCR | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Word Count Estimation | 10,169 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 40192-2021: Detection and identification of Colletotrichum Corda using real-time PCR---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Colletotrichum Corda using real-time PCR
ICS 65.020.01
CCSB16
National Standards of People's Republic of China
Real-time fluorescent PCR quarantine and identification method for Colletotrichum
Released on 2021-05-21
2021-12-01 implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents.
This document was proposed and managed by the National Plant Quarantine Standardization Technical Committee (SAC/TC271).
Drafting organizations of this document. Ningbo Customs of the People's Republic of China, Institute of Microbiology, Chinese Academy of Sciences, Urumqi, People's Republic of China
Customs, China Academy of Inspection and Quarantine.
The main drafters of this document. Duan Weijun, Cai Lei, Liu Fang, Li Xuelian, Wang Chong, Zhao Peng, Guo Lixin, Zhang Huili, Zhang Yongjiang, Chen Xianfeng.
Real-time fluorescent PCR quarantine and identification method for Colletotrichum
1 Scope
This document describes the real-time fluorescent PCR quarantine and identification method for the plant pathogenic fungus Colletotrichum spp.
This document is applicable to the detection and screening of Colletotrichum spp. in plants and products that carry Colletotrichum spp.
2 Normative references
The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations
Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
This document.
SN/T 2589 Test specification for plant pathogenic fungi
3 Terms and definitions
There are no terms and definitions that need to be defined in this document.
4 Classification information of Colletotrichum spp
Chinese name. Colletotrichum spp.
Scientific name. Colletotrichum Corda.
According to the "Dictionary of the Fungi" (10th edition) (2008) classification system, Colletotrichum spp belongs to the kingdom of Fungi (Fungi).
Ascomycota, Sordariomycetes, Glomerelales, Glomerelaceae.
Transmission route. Colletotrichum spp. is mainly spread by seeds or diseased bodies. It can also be spread by wind, rain and insects.
5 Principles of Identification
According to the characteristics of the DNA sequence of Colletotrichum spp., real-time fluorescent PCR method is used to apply the designed primers and probes to Colletotrichum spp.
The genus pathogens are tested and identified.
6 Equipment, utensils, reagents and culture medium
6.1 Instruments and appliances
Autoclave, microscope, dissecting mirror, balance, water bath pure water instrument, vortex oscillator, ultraviolet spectrophotometer, high-speed refrigerated centrifuge,
Refrigerator, biochemical incubator, real-time fluorescent PCR machine, micropipette (0.1μL~2.5μL, 1μL~10μL, 10μL~50μL, 20μL~
200μL, 100μL~1000μL), Erlenmeyer flask, Petri dish, glass slide, cover glass.
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