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GB/T 40187-2021: Technical guidance for affinity and specific of aptamer
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Technical guidance for affinity and specific of aptamer
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GB/T 40187-2021
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Basic data | Standard ID | GB/T 40187-2021 (GB/T40187-2021) | | Description (Translated English) | Technical guidance for affinity and specific of aptamer | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A20 | | Word Count Estimation | 6,684 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 40187-2021: Technical guidance for affinity and specific of aptamer---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Technical guidance for affinity and specific of aptamer
ICS 07.080
A20
National Standards of People's Republic of China
Technical Guidelines for Evaluation of Affinity and Specificity of Nucleic Acid Aptamers
Released on 2021-05-21
2021-12-01 implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is under the jurisdiction of the China National Institute of Standardization.
Drafting organizations of this standard. Hebei Food Inspection and Research Institute, China National Institute of Standardization, Jiangnan University, Hefei University of Technology, China Science and Technology
University, Hebei Agricultural University, Southwest University, Zhejiang Gongshang University, Henan University, Beijing Institute of Food Science, Hebei Medical University.
The main drafters of this standard. Zhang Yan, Zhou Wei, Ma Aijin, Wang Zhouping, Qu Hao, Luo Zhaofeng, Zheng Lei, Wang Xianghong, Chen Jia, Ma Liang, Tian Yiling,
Wang Zan, Zhang Yalun, Zhang Tao, Kang Wenwen, Wang Yanbo, Fu Linglin, Sun Yong, Chen Wei, Lu Pin.
Technical Guidelines for Evaluation of Affinity and Specificity of Nucleic Acid Aptamers
1 Scope
This standard specifies the evaluation rules and determination methods for the binding affinity and specificity of nucleic acid aptamers to the target.
This standard applies to different levels of target objects such as metal ions, small molecules, macromolecules, cells and particles, tissues, and mixed targets.
Evaluation of the affinity and specificity of the nucleic acid aptamer.
2 Normative references
The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article
Pieces. For undated reference documents, the latest version (including all amendments) is applicable to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB/T 27403 Laboratory Quality Control Specification Food Molecular Biology Testing
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Affinity
The gravitation that exists between the specific target and the nucleic acid aptamer is the inherent binding force of the specific target and the nucleic acid aptamer.
Note. The strength of affinity is generally expressed by the equilibrium dissociation constant KD.
3.2
Specificity
Under the same measurement method, the same amount and concentration of the substance, determine the binding number of the nucleic acid aptamer to the target and its non-target
Note. The higher the ratio of binding quantity, the better the specificity.
3.3
Equilibrium dissociation constant
When the nucleic acid aptamer binds to the target to reach the dissociation equilibrium, the product of the power function of the concentration of each product divided by the power function of the reactant concentration.
Note. In most cases, the nucleic acid aptamer and the target are bound 1.1, and the equilibrium dissociation constant of the nucleic acid aptamer can be simplified to the free state in solution
The product of the concentration of aptamer and the concentration of the target substance in the free state is divided by the concentration of the two complexes. Is numerically equal to the target substance
The concentration (KD) of the target substance when the aptamer binds halfway. The smaller the KD value, the stronger the interaction between the two.
KD=
cAcT
cE
Where.
KD --- equilibrium dissociation constant;
cA --- the amount and concentration of the nucleic acid aptamer;
cT --- the amount and concentration of the target molecular substance;
cE --- The amount and concentration of the compound's substance.
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