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GB/T 40183-2021 English PDF

GB/T 40183-2021_English: PDF (GB/T40183-2021)
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GB/T 40183-2021English119 Add to Cart 3 days [Need to translate] Determination of DNA methylation - Pyrosequencing Valid GB/T 40183-2021


BASIC DATA
Standard ID GB/T 40183-2021 (GB/T40183-2021)
Description (Translated English) Determination of DNA methylation - Pyrosequencing
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard A21
Classification of International Standard 07.080
Word Count Estimation 6,652
Date of Issue 2021-05-21
Date of Implementation 2021-12-01
Drafting Organization Hebei Medical University, China National Institute of Standardization, Hebei Food Inspection and Research Institute, Agricultural Resources Research Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Hebei Normal University
Administrative Organization China Design and Research Institute
Regulation (derived from) National Standard Announcement No. 7 of 2021
Proposing organization China National Institute of Standardization
Issuing agency(ies) State Administration for Market Regulation, National Standardization Administration


GB/T 40183-2021 Determination of DNA methylation-Pyrosequencing ICS 07.080 A21 National Standards of People's Republic of China DNA methylation determination by pyrosequencing Released on 2021-05-21 2021-12-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee Preface This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard was proposed and managed by the China National Institute of Standardization. Drafting organizations of this standard. Hebei Medical University, China National Institute of Standardization, Hebei Food Inspection and Research Institute, Chinese Academy of Sciences Genetics and Development Agricultural Resources Research Center, Institute of Educational Biology, Hebei Normal University. The main drafters of this standard. Lu Pin, Ma Aijin, Zhang Yan, Lin Hao, Li Yongpeng, Li Dongming, Huang Yun, Yang Hongchao. DNA methylation determination by pyrosequencing 1 Scope This standard specifies the method for the determination of DNA methylation by pyrosequencing. This standard applies to the determination of DNA methylation in animal and plant tissues or cells cultured in vitro. 2 Normative references The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article Pieces. For undated reference documents, the latest version (including all amendments) is applicable to this document. GB/T 6682 Analytical laboratory water specifications and test methods 3 Terms and definitions, abbreviations 3.1 Terms and definitions The following terms and definitions apply to this document. 3.1.1 DNA methylation Under the catalysis of DNA methyltransferase, S-adenosylmethionine is used as the methyl donor to transfer the methyl group to the specific structure of the base. process. 3.2 Abbreviations The following abbreviations apply to this document. DNA. deoxyribonucleic acid (deoxyribonucleicacid) PCR. polymerase chain reaction (polymerase chain reaction) 4 Principle After the DNA is vulcanized by bisulfate, the unmethylated cytosine in the DNA double strand is converted into uracil, and the uracil is converted by PCR. Converted to thymine, quantitatively based on the ratio of cytosine and thymine at the corresponding methylation sites in the PCR product. 5 Reagents or materials Unless otherwise specified, only use analytical reagents. 5.1 Water. GB/T 6682, Grade II water. 5.2 Animal genomic DNA extraction kit. 5.3 Plant genomic DNA extraction kit. 5.4 20pmol/μL pyrosequencing primer. 5.5 PCR kit. ......

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