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GB/T 40176-2021: Determination of xylobiose in plant-derived products - Hydrophilic intention chromatography
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 40176-2021 | English | 189 |
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Determination of xylobiose in plant-derived products - Hydrophilic intention chromatography
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GB/T 40176-2021
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Basic data | Standard ID | GB/T 40176-2021 (GB/T40176-2021) | | Description (Translated English) | Determination of xylobiose in plant-derived products - Hydrophilic intention chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | A40 | | Word Count Estimation | 10,185 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 40176-2021: Determination of xylobiose in plant-derived products - Hydrophilic intention chromatography
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Determination of xylobiose in plant-derived products-Hydrophilic intention chromatography
ICS 07.080
CCSA40
National Standards of People's Republic of China
Determination of Xylobiose in Plant-derived Products
Hydrophilic Retention Chromatography
Released on 2021-05-21
2021-12-01 implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents.
This document is under the jurisdiction of the National Standardization Technical Committee for Biochemical Testing (SAC/TC387).
Drafting organizations of this document. Jiangnan University, Institute of Agricultural Products Processing, Chinese Academy of Agricultural Sciences, Beijing Technology and Business University, Beijing Sambo Technology Co., Ltd.
Co., Ltd., Huapu Science Instrument (Beijing) Technology Co., Ltd., Lijin County Inspection and Testing Center.
The main drafters of this document. Xu Chuanlai, Wang Feng, Ma Aijin, Gu Fengying, Hao Shuai, Li Bo, Zhu Jinjin, Xu Runqi, Ding Yanan, Liu Peiyu,
Li Xiuting and Li Peng.
Determination of Xylobiose in Plant-derived Products
Hydrophilic Retention Chromatography
1 Scope
This document specifies the method for the determination of xylobiose in plant-derived products by HPLC.
This document is applicable to the determination of xylobiose in plant-derived products.
2 Normative references
The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations
Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
This document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Terms and definitions
There are no terms and definitions that need to be defined in this document.
4 Principle
The sample is extracted with water to remove impurities such as fat, protein, starch, non-starch sugar, and the reducing end of xylobiose is combined with 1-phenyl-3-methyl-5-pyrazole
The derivatization reaction of PMP occurs to produce a stable product with strong ultraviolet absorption, which is detected by high-performance liquid hydrophilic retention chromatography.
Measured and quantified by external standard method.
5 Reagents and materials
Unless otherwise specified, the reagents used in this method are all analytically pure, and the water is the first grade water specified in GB/T 6682.
5.1 Reagents
5.1.1 Acetonitrile (CH3CN). chromatographically pure.
5.1.2 Anhydrous ethanol (CH3CH2OH).
5.1.3 Acetone (CH3COCH3).
5.1.4 Methanol (CH3OH).
5.1.5 1-Phenyl-3-methyl-5-pyrazolone (PMP) (C10H10N2O).
5.1.6 Trichloromethane (CHCl3).
5.1.7 Hydrochloric acid (HCl).
5.1.8 Sodium hydroxide (NaOH).
5.1.9 Phosphoric acid (H3PO4). chromatographically pure.
5.1.10 Xylobiose (C10H18O9, CAS number. 6860-47-5). Purity ≥99.0%.
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