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GB/T 40152-2021: Determination of saccharase activity in honey - Spectrophotometric method
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 40152-2021 | English | 119 |
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Determination of saccharase activity in honey - Spectrophotometric method
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GB/T 40152-2021
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Basic data | Standard ID | GB/T 40152-2021 (GB/T40152-2021) | | Description (Translated English) | Determination of saccharase activity in honey - Spectrophotometric method | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X31 | | Word Count Estimation | 6,646 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 40152-2021: Determination of saccharase activity in honey - Spectrophotometric method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of saccharase activity in honey-Spectrophotometric method
ICS 67.180.10
CCSX31
National Standards of People's Republic of China
Determination of sucrose invertase in honey
Released on 2021-05-21
2021-12-01 implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents.
This document was proposed by the All-China Federation of Supply and Marketing Cooperatives.
This document is under the jurisdiction of the National Bee Product Standardization Working Group (SAC/SWG2).
This document was drafted by. Jiangsu Zhongpu Testing Co., Ltd.
The main drafters of this document. Xu Jinzhong, Yang Xiqun, Lu Huiling, Wang Yingjun, Chen Mei, Wang Yan, Wang Min, Yao Chao.
Determination of sucrose invertase in honey
1 Scope
This document specifies the spectrophotometric method for the determination of sucrose invertase in honey.
This document is applicable to the determination of sucrose invertase in honey.
2 Normative references
The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations
Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to
This document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Terms and definitions
There are no terms and definitions that need to be defined in this document.
4 Principles of the method
The sucrose invertase contained in honey can quantitatively convert the substrate p-nitrobenzene-α-D-glucopyranoside into p-nitrophenol, and pass the test
Determine the absorbance value at a specific wavelength to determine the content of p-nitrophenol to determine the sucrose invertase in honey. Add honey solution to substrate solution
Mix well. After reacting at 40°C for 20 minutes, add a stop solution to terminate the reaction. Measure the absorbance at a wavelength of 400nm.
The absorbance value is directly proportional to the sucrose invertase value. At the same time, make a blank control.
5 Reagents and materials
Unless otherwise specified, the reagents used in this method are all analytically pure, and the water is the third grade water specified in GB/T 6682.
5.1 Potassium dihydrogen phosphate (KH2PO4).
5.2 Disodium hydrogen phosphate dihydrate (Na2HPO4·2H2O).
5.3 p-Nitrophenyl-α-D-glucopyranoside (CASNO. 3767-28-0).
5.4 Tris.
5.5 0.1mol/L phosphate buffer (pH 6.0).
Weigh 11.66g potassium dihydrogen phosphate and 2.56g disodium hydrogen phosphate dihydrate (Na2HPO4·2H2O), dissolve in water, and dilute to 1L.
5.6 0.02mol/L substrate solution (pH 6.0).
Weigh 6.025g of p-nitrophenyl-α-D-glucopyranoside, add about 900mL of phosphate buffer (5.5) to dissolve, and if necessary, heat to
Solubility at 60℃, cool to room temperature, dilute to 1L, store at 4℃ in the dark, valid period 7d.
5.7 3mol/L stop solution.
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