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GB/T 40141-2021: Detection and identification of Candidatus Phytoplasma ulmi
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Detection and identification of Candidatus Phytoplasma ulmi
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GB/T 40141-2021
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Basic data | Standard ID | GB/T 40141-2021 (GB/T40141-2021) | | Description (Translated English) | Detection and identification of Candidatus Phytoplasma ulmi | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Word Count Estimation | 14,153 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | GB/T 40141-2021: Detection and identification of Candidatus Phytoplasma ulmi---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Candidatus Phytoplasma ulmi
ICS 65.020.01
CCSB16
National Standards of People's Republic of China
Quarantine and identification methods for phytoplasma of phloem necrosis of elm
Released on 2021-05-21
2021-12-01 implementation
State Administration of Market Supervision and Administration
Issued by the National Standardization Management Committee
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents"
Drafting.
Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents.
This document was proposed and managed by the National Plant Quarantine Standardization Technical Committee (SAC/TC271).
Drafting organizations of this document. Chinese Academy of Inspection and Quarantine Sciences, Inner Mongolia Agricultural University, Qingdao Customs Technology Center, Shanghai Agricultural Technology
Promotion service center.
The main drafters of this document. Zhao Wenjun, Tian Qian, Li Zhengnan, Zhang Hongmei, Mou Haiqing, Zhang Jingxuan, Luo Jinyan.
Quarantine and identification methods for phytoplasma of phloem necrosis of elm
1 Scope
This document describes the quarantine and identification methods for phytoplasma of phloem necrosis of elm.
This document is applicable to the quarantine and identification of phytoplasma of the phloem necrosis of the elm tree in and out of the country.
2 Normative references
There are no normative references in this document.
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Polymerase chain reaction polymerase chain reaction; PCR
Heat-resistant DNA polymerase and a pair of primers (DNA fragments homologous to the target nucleic acid molecule sequence to be tested) are passed through high temperature (DNA analysis).
(Subdenaturation) and low temperature (primers and target nucleic acid molecules are renatured and extended by heat-resistant DNA polymerase) alternately cyclically amplify the target nucleic acid to be tested
Sub-method.
3.2
Phytoplasma
The first type has no cell wall and cannot be artificially cultured. It is mainly distributed in the phloem sieve cells of plants, the intestinal tract of piercing and sucking mediator insects, lymph,
Salivary glands and other tissues.
4 Basic information of phytoplasma of phloem necrosis of elm
Scientific name. CandidatusPhytoplasmaulmi
Transmission route. Rely on diseased seedlings for long-distance transmission.
See Appendix A for additional information on the phytoplasma of the phloem necrosis of the elm.
5 Principles of the method
The disease mainly harms elm trees, and it is spread over long distances by diseased seedlings. Molecular biology such as its biological characteristics and 16S gene sequence
Scientific information is the basis of this quarantine identification method. 16SrDNA is relatively conservative and can be amplified by phytoplasma universal primers R16F2/R16R2
The 16SrDNA sequence of phytoplasma was obtained.
6 Instruments and main reagents
6.1 Equipment
PCR instrument, ultra-clean workbench, sterilization pot, high-speed refrigerated centrifuge, desktop small centrifuge, ultra-low temperature refrigerator, conventional refrigerator, vortex oscillation
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