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GB/T 39303-2020: General technical specification for pre-treatment of microorganism samples in wastewater treatment system
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| GB/T 39303-2020 | English | 209 |
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General technical specification for pre-treatment of microorganism samples in wastewater treatment system
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GB/T 39303-2020
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Basic data | Standard ID | GB/T 39303-2020 (GB/T39303-2020) | | Description (Translated English) | General technical specification for pre-treatment of microorganism samples in wastewater treatment system | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | G76 | | Classification of International Standard | 19.020; 07.100.01 | | Word Count Estimation | 11,149 | | Date of Issue | 2020-11-19 | | Date of Implementation | 2021-10-01 | | Quoted Standard | HJ 91.1; SN/T 2632 | | Regulation (derived from) | National Standard Announcement No. 26 of 2020 | | Issuing agency(ies) | State Administration for Market Regulation, China National Standardization Administration | | Summary | This standard specifies the on-site treatment of microbial samples in wastewater treatment systems, the separation and preservation of microbial strains, the extraction of metagenomic DNA from microbial samples, and the preservation of microbial samples. This standard applies to the on-site treatment, extraction and preservation of microbial samples in wastewater treatment systems using biological treatment processes (activated sludge process and biofilm process). | GB/T 39303-2020: General technical specification for pre-treatment of microorganism samples in wastewater treatment system
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(General technical specifications for pretreatment of microbial samples in wastewater treatment systems)
ICS 19.020;07.100.01
G76
National Standards of People's Republic of China
Microbial sample pretreatment of wastewater treatment system
General technical specifications
2020-11-19 released
2021-10-01 implementation
State Administration for Market Regulation
Issued by the National Standardization Management Committee
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard was proposed by the China Petroleum and Chemical Industry Federation.
This standard is under the jurisdiction of the National Chemical Standardization Technical Committee (SAC/TC63).
Drafting organizations of this standard. Nanjing University, Nanjing University Yixing Environmental Protection Research Institute, Nanjing Jiangdao Environmental Technology Research Institute Co., Ltd., CNOOC
Tianjin Chemical Research and Design Institute Co., Ltd., Lanbao (Xiamen) Water Treatment Technology Co., Ltd., Dongguan Institute of Technology, State Grid Tianjin Electric Power Company
Electric Power Research Institute, Shijiazhuang Geyuan Environmental Protection Technology Co., Ltd., and Zhuhai Jinggong Testing Technology Co., Ltd.
The main drafters of this standard. Ren Hongqiang, He Xiwei, Zhang Xuxiang, Bai Ying, Lu Fenyong, Niu Junfeng, Su Zhan, Geng Jinju, Li Yongguang, Wang Qing,
Wang Lei, Wu Bing.
Microbial sample pretreatment of wastewater treatment system
General technical specifications
1 Scope
This standard specifies the on-site treatment of microbial samples in wastewater treatment systems, the separation and preservation of microbial strains, and the metagenes of microbial samples
Group DNA extraction and preservation of microbial samples.
This standard applies to microbial samples in wastewater treatment systems using biological treatment processes (activated sludge process and biofilm process)
On-site processing, extraction and storage.
2 Normative references
The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article
Pieces. For undated references, the latest version (including all amendments) applies to this document.
HJ91.1 Technical Specification for Wastewater Monitoring
SN/T 2632 Technical regulations for conventional preservation of microbial strains
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Microbiological samples
Various strains (including bacteria, fungi, actinomycetes, etc.), DNA, etc. that have been cultured or extracted and purified and can be directly applied to analysis and research
Genetic material; and water samples, activated sludge samples, biofilm samples in wastewater treatment systems containing the above-mentioned strains and genetic materials
Pin etc.
3.2
Pretreatment
Various treatments of various microbial samples collected before carrying out the research of microorganisms in the wastewater treatment system.
3.3
The genetic material (DNA or RNA) extracted directly from environmental samples without strain isolation and screening steps contains all the microbes in the sample.
The genetic information of organisms.
[GB/T 30744-2014, definition 3.1.6]
4 Abbreviations
The following abbreviations apply to this document.
CTAB. Cetyl Trimethyl Ammonium Bromide
DNA. deoxyribonucleic acid
EDTA. ethylenediaminetetraacetic acid
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