GB/T 35252-2017 (GBT 35252-2017)

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GB/T 35252-2017	English	199	Add to Cart	3 days	Animal and vegetable fats and oils -- Determination of 2-thiobarbituric acid value -- Direct method	GB/T 35252-2017	Valid	GB/T 35252-2017

GB/T 35252-2017
Animal and vegetable fats and oils - Determination of 2-thiobarbituric acid value - Direct method
ICS 67.200.10
X14
National Standards of People's Republic of China
Animal and vegetable oils and fats
Determination of 2 - thiobarbituric acid value of the direct method
2017-12-29 Posted
2018-07-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
China National Standardization Administration released
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard proposed by the State Food Administration.
This standard by the National Cereals and Oils Standardization Technical Committee (SAC/TC270) centralized.
This standard was drafted by the State Food Administration of Science.
Drafters of this standard. Luan Xia, Wang Ying Yao, Ma Rong, Zhang Fan, Feng Ren-dong.
Animal and vegetable oils and fats
Determination of 2 - thiobarbituric acid value of the direct method
1 Scope
This standard specifies the method of direct determination of 2-thiobarbituric acid (TBA value) without the separation of secondary oxidation products of fat.
This standard applies to animal and vegetable oils and fats, fatty acids and esters, glycol esters and so on.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
Laboratory use specifications and test methods for analysis GB/T 6682-2008
GB/T 15687 Animal and vegetable fats sample preparation
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
2-thiobarbituric acid value 2-thiobarbituric acid value
The absorbance, measured at 530 nm, of a 1 mg sample reacted with 1 mL of 2-thiobarbituric reagent.
Note 2 - thiobarbituric acid value without units, but 1mg sample dissolved in 100mL solvent and reagent measured absorbance value of a measurement
unit.
4 principle
The secondary oxidation product of the oil reacts with 2-thiobarbituric acid to form a condensate having a maximum absorption peak at a wavelength of 530 nm,
Its absorbance.
5 reagent
5.1 1-butanol. Moisture content of not more than 0.2%.
5.2 2-thiobarbituric acid.
5.3 TBA Reagents. Weigh.200 mg of 2-thiobarbituric acid in a 100 mL volumetric flask and dilute to the mark with 1-butanol. in room temperature
Place 12h ~ 15h to dissolve 2-thiobarbituric acid. Then filter with filter paper to remove insoluble residue. The filtrate was titrated with 1-butanol
100mL, and stored at 0 ℃ ~ 4 ℃ refrigerator spare. TBA reagents are stored and used for less than 1 week.
5.4 All reagents are of analytical grade, the experimental water should meet the requirements of GB/T 6682-2008 three water.
6 equipment
6.1 volumetric flask .25mL, 100mL.