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GB/T 33309-2016: Determination of vitamin B6 (pyridoxine, pyridoxine hydrochloride, pyridoxine fatty acid esters and pyridoxal 5-phosphate) in cosmetics -- High performance liquid chromatography
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Determination of vitamin B6 (pyridoxine, pyridoxine hydrochloride, pyridoxine fatty acid esters and pyridoxal 5-phosphate) in cosmetics -- High performance liquid chromatography
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GB/T 33309-2016
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PDF similar to GB/T 33309-2016
Basic data | Standard ID | GB/T 33309-2016 (GB/T33309-2016) | | Description (Translated English) | Determination of vitamin B6 (pyridoxine, pyridoxine hydrochloride, pyridoxine fatty acid esters and pyridoxal 5-phosphate) in cosmetics -- High performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Classification of International Standard | 71.100.70 | | Word Count Estimation | 10,125 | | Date of Issue | 2016-12-13 | | Date of Implementation | 2017-07-01 | | Regulation (derived from) | National Standard Notice No.264 of 2016 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 33309-2016: Determination of vitamin B6 (pyridoxine, pyridoxine hydrochloride, pyridoxine fatty acid esters and pyridoxal 5-phosphate) in cosmetics -- High performance liquid chromatography
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Determination of vitamin B6 (pyridoxine, pyridoxine hydrochloride, pyridoxine fatty acid esters and pyridoxal 5-phosphate) in cosmetics - High performance liquid chromatography
ICS 71.100.70
Y42
National Standards of People's Republic of China
Cosmetics in the vitamin B6 (pyridoxine, pyridoxine hydrochloride,
Pyridoxine fatty acid esters and pyridoxal 5-phosphate esters)
Determination of High Performance Liquid Chromatography
Determination of vitaminB6 (pyridoxine, pyridoxinehydrochloride,
pyridoxinefattyacidestersandpyridoxal5-phosphate) incosmetics-
2016-12-13 released
2017-07-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Management Committee released
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed by the China Light Industry Federation.
This standard by the National Spice Fragrance Cosmetics Standardization Technical Committee (SAC/TC257) centralized.
The drafting unit of this standard. Shandong Province Product Quality Supervision and Inspection Research Institute, Liaocheng University, Shandong Province Food and Drug Inspection Research Institute, Shanghai
Institute of Daily Chemical Industry.
The main drafters of this standard. Huo Yanmin, Wang Jun, Zhang Juan, Zhang Hui, Zhou Lili, Duan Wendeng, Zou Huiling, Wang Yanli, Xue Xia,
Liu Yanming, Cui Yuhua, Liu Guiliang, Zhang Yanxia, Shen Min.
Cosmetics in the vitamin B6 (pyridoxine, pyridoxine hydrochloride,
Pyridoxine fatty acid esters and pyridoxal 5-phosphate esters)
Determination of High Performance Liquid Chromatography
1 Scope
This standard specifies the high levels of vitamin B6 (pyridoxine, pyridoxine hydrochloride, pyridoxine fatty acid ester and pyridoxal 5-phosphate) in cosmetics
Determination of liquid chromatography.
This standard applies to cream, lotion, liquid and other cosmetics in the pyridoxine, pyridoxine hydrochloride, pyridoxine fatty acid ester and pyridoxal 5-phosphorus
Determination of acid esters.
The detection limit of this standard method is. pyridoxal 1.0mg/kg, pyridoxine 0.3mg/kg, the quantitative limit is. pyridoxal 2.5mg/kg, pyridoxine
1.2mg/kg.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article
Pieces. For undated references, the latest edition (including all modifications) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
3 principle
This standard through the enzymolysis, saponification reaction in the sample of vitamin B6 are converted to pyridoxine or pyridoxal form, the use of high-performance liquid
Chromatography with fluorescence detector for separation and analysis, retention time qualitative, external standard method for quantitative determination of vitamin B6 content.
4 reagents and materials
Unless otherwise stated, the reagents used were of analytical grade and water was a primary water specified in GB/T 6682.
4.1 methanol. pure chromatography.
4.2 heptafluorobutyric acid. pure chromatography.
4.3 alkaline phosphatase. enzyme activity of not less than 10U/mg.
4.4 phosphoric acid. excellent grade pure.
4.5 ammonia. excellent grade pure, content of 25% to 28%.
4.6 ammonium dihydrogen phosphate. excellent grade pure.
4.7 Disodium hydrogen phosphate (Na2HPO4 · 2H2O).
4.8 Ethanol.
4.9 Hydrochloric acid.
4.10 aqueous solution of potassium hydroxide. Weigh 250g of solid potassium hydroxide and add.200 mL of water to dissolve.
4.11 30% phosphoric acid aqueous solution (volume fraction). Accurately measure 30mL phosphoric acid (4.4) and 70mL water, mix and spare.
4.12 30% ammonia solution (volume fraction). Accurately measure 30mL ammonia (4.5) and 70mL water, mix and spare.
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