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GB/T 33127-2016: Detection and identification of Sugarcane streak virus
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| GB/T 33127-2016 | English | 279 |
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Detection and identification of Sugarcane streak virus
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GB/T 33127-2016
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Basic data | Standard ID | GB/T 33127-2016 (GB/T33127-2016) | | Description (Translated English) | Detection and identification of Sugarcane streak virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 14,196 | | Date of Issue | 2016-10-13 | | Date of Implementation | 2017-05-01 | | Regulation (derived from) | National Standard Notice No.1716 of 2016 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 33127-2016: Detection and identification of Sugarcane streak virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Sugarcane streak virus
ICS 65.020.01
B16
National Standards of People's Republic of China
Identification method of sugarcane line virus
2016-10-13 released
2017-05-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Management Committee released
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed by the National Commission for Standardization of Plant Quarantine Standardization (SAC/TC271).
The drafting unit of this standard. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the People's Republic of China Hainan Entry-Exit Inspection
Epidemic situation.
The main drafters of this standard. Feng Lixia, Wu Zhentao, Liu Fuxiu, Zhao Lirong, Wang Weifang, Yang Lei Liang.
Identification method of sugarcane line virus
1 Scope
This standard specifies the method of quarantine identification of sugarcane line virus.
This standard applies to quarantine identification of living host plants that may carry sugarcane line virus.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article
Pieces. For undated references, the latest edition (including all modifications) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
SN/T 1840 Plant virus immunoelectron microscopy
SN/T 2122 Quarantine sampling of inbound and outbound plants and plant products
3 sugar cane line virus basic information
Chinese name. sugar cane line virus.
Scientific name. Sugarcanestreakvirus.
Abbreviations. SSV.
Taxonomic status. Geminiviridae, Mastrevirus.
See Appendix A for additional information on sugarcane line viruses.
4 Principle of the method
SSV immunogenicity and genomic characteristics are the basis for the identification of the virus. Using conventional PCR, real-time PCR, double antibody
Sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and immunoelectron microscopy were used to detect the samples.
5 Instruments, facilities, utensils and reagents
5.1 Instruments
Electronic balance (inductance 0.001g), high-speed centrifuge, small instant centrifuge, ultra-low temperature refrigerator (-80 ℃), ice machine, vortex oscillation
, Magnetic stirrer, autoclave, pH meter, transmission electron microscope, clean bench, PCR instrument, real-time fluorescence quantitative PCR instrument, micro
Furnace, electrophoresis, electrophoresis tank, gel imaging analyzer, enzyme detector.
5.2 facilities
Isolated greenhouse (10 ℃ ~ 30 ℃).
5.3 appliances
(2.5 μL, 10 μL, 20 μL, 100 μL, 1000 μL) and an adjustable pipette head, centrifuge tube, mortar, miniature
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