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GB/T 33120-2016: Detection and identification of Pear blister canker viroid
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 33120-2016 | English | 279 |
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Detection and identification of Pear blister canker viroid
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GB/T 33120-2016
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Basic data | Standard ID | GB/T 33120-2016 (GB/T33120-2016) | | Description (Translated English) | Detection and identification of Pear blister canker viroid | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 14,165 | | Date of Issue | 2016-10-13 | | Date of Implementation | 2017-05-01 | | Regulation (derived from) | National Standard Notice No.1716 of 2016 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 33120-2016: Detection and identification of Pear blister canker viroid---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Pear blister canker viroid
ICS 65.020.01
B16
National Standards of People's Republic of China
Identification method of psoriasis virus
2016-10-13 released
2017-05-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Management Committee released
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The release of this document
The Agency does not assume responsibility for identifying these patents.
This standard is proposed by the National Commission for Standardization of Plant Quarantine Standardization (SAC/TC271).
The drafting unit of this standard. Hubei Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Zhongshan Entry and Exit Inspection and Quarantine of the People's Republic of China
Bureau of the People's Republic of China Xinjiang Entry-Exit Inspection and Quarantine Bureau, the People's Republic of China Yunnan Entry-Exit Inspection and Quarantine Bureau, the People's Republic of China
Shandong Entry - Exit Inspection and Quarantine Bureau, the People's Republic of China Inspection and Quarantine Science Research Institute, Huazhong Agricultural University College of Plant Science and Technology.
The main drafters of this standard. Wang Zhenhua, Chen Dinghu, Ye Yun, Xu Wenxing, Xu Jiawen, Zeng Xiandong, Xu Xue, Zhang Yongjiang, Wang Chong, Li Min, Wang Yingchao,
Wu Xinghai.
Identification method of psoriasis virus
1 Scope
This standard specifies the method of identification of psoriasis virus.
This standard applies to the import and export of pear tree seedlings, tissue culture seedlings and plant pear-like ulcer virus quarantine and identification.
2 pear blister ulcer virus basic information
Scientific name. Pearblistercankerviroid
Abbreviations. PBCVd.
Synonyms. Papilla virus.
Classification Status. Potato Spindle Bacteria Virus (Pospiviroidae), Apple Rust Virus (Apscaviroid).
Transmission. through grafting, budding and tooling mechanical transmission, but no reports of mediators and seeds. Long distance through the seedlings
Transportation.
See Appendix A for additional information on psoriasis virus.
3 Principle of the method
Based on the conserved region of the full-length RNA nucleic acid strand of the pear-like ulcer virus, a pair of amplifiers for the full length of the virus
RT-PCR amplification and sequencing; according to pear-type ulcer virus full-length nucleic acid sequence design and single-stranded RNA complementary single
Chain RNA probe, through the hybrid signal strength to determine the results.
4 equipment and main reagents
4.1 Instruments and equipment
Clean bench, sterilization pot, ice maker, high speed refrigerated centrifuge, ultra low temperature refrigerator, conventional refrigerator, vortex oscillator, micro sampler, vertical
Direct electrophoresis tank, PCR instrument, gel imaging system, hybrid furnace, cross-linked instrument, hybrid tube, chemiluminescence detector.
4.2 Main reagents
Unless otherwise specified, all reagents are of analytical grade. RT-PCR reagents see Appendix B; molecular hybrid reagents see Appendix C.
5 symptoms observed
Sample samples of 1% to 5% (if the sample is less likely to increase the proportion of) seedlings to check. Check the psoriasis virus infection
Shaped, bark on the formation of blister or bark cracking, ulcers, squamous epidermis or epidermis deep, tree potential decline.
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