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GB/T 33114-2016: Detection and identification of Prunus necrotic ringspot virus
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| GB/T 33114-2016 | English | 279 |
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Detection and identification of Prunus necrotic ringspot virus
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GB/T 33114-2016
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Basic data | Standard ID | GB/T 33114-2016 (GB/T33114-2016) | | Description (Translated English) | Detection and identification of Prunus necrotic ringspot virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 14,140 | | Date of Issue | 2016-10-13 | | Date of Implementation | 2017-05-01 | | Regulation (derived from) | National Standard Notice No.1716 of 2016 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 33114-2016: Detection and identification of Prunus necrotic ringspot virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Prunus necrotic ringspot virus
ICS 65.020.01
B16
National Standards of People's Republic of China
Identification method of necrotic ring spot virus
2016-10-13 released
2017-05-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Management Committee released
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The release of this document
The Agency does not assume responsibility for identifying these patents.
This standard is proposed by the National Commission for Standardization of Plant Quarantine Standardization (SAC/TC271).
The drafting of this standard. China Inspection and Quarantine Science Research Institute, the People's Republic of China Hunan Entry-Exit Inspection and Quarantine Bureau, Fujian Province Agriculture
Academy.
The main drafters of this standard. Zhang Yongjiang, Huang Yingbo, Kong Jun, Xie Lixue, Li Guifen, Ma Jie, Xin Yizhen, Wei Meisheng.
Identification method of necrotic ring spot virus
1 Scope
This standard specifies the method of quarantine identification of Prunusnecroticringspotvirus.
This standard applies to quarantine identification of plants and their products that may carry the genus Nematocysts.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article
Pieces. For undated references, the latest edition (including all modifications) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
SN/T 2122 Quarantine sampling of inbound and outbound plants and plant products
Code for the detection of plant viruses
Code for molecular biology detection of plant viruses
3 is a necrotic ring spot virus basic information
Scientific name. Prunusnecroticringspotvirus
Abbreviations. PNRSV
Taxonomic status. Bromoviridae, Ilarvirus.
For other information, see Appendix A.
4 Principle of the method
The serological and genomic characteristics of the necrotic ring spot virus are the basis for the identification of the virus. According to PNRSV with antibodies
(DAS-ELISA); the gene based on PNRSV was used to isolate the plant samples by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA)
Group of RT-PCR, real-time fluorescence RT-PCR detection method; through the effective combination of these methods to determine whether the sample with
PNRSV.
5 instruments, utensils and reagents
5.1 Instruments
Electronic balance (inductance 0.001g), high-speed refrigerated centrifuge, small instant centrifuge, ordinary refrigerator, ultra-low temperature refrigerator (-80 ℃), the system
Ice machine, vortex oscillator, magnetic stirrer, autoclave, pH meter, clean bench, PCR instrument, microwave oven, electrophoresis, electrophoresis tank, gel
Imaging analyzer, microplate reader.
5.2 Appliances
(2.5 μL, 10 μL, 20 μL, 100 μL,.200 μL, 1000 μL) and an adjustable pipette head, Eppendorf
Tube, mortar, micro-grinding pestle and so on.
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