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GB/T 33108-2016: Determination of sea cucumber glycosides in sea cucumber and sea cucumber products -- High performance liquid chromatography
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Determination of sea cucumber glycosides in sea cucumber and sea cucumber products -- High performance liquid chromatography
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GB/T 33108-2016
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Basic data | Standard ID | GB/T 33108-2016 (GB/T33108-2016) | | Description (Translated English) | Determination of sea cucumber glycosides in sea cucumber and sea cucumber products -- High performance liquid chromatography | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B51 | | Classification of International Standard | 67.120 | | Word Count Estimation | 10,115 | | Date of Issue | 2016-10-13 | | Date of Implementation | 2017-05-01 | | Regulation (derived from) | National Standard Notice No.1716 of 2016 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 33108-2016: Determination of sea cucumber glycosides in sea cucumber and sea cucumber products -- High performance liquid chromatography
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Determination of sea cucumber glycosides in sea cucumber and sea cucumber products - High performance liquid chromatography
ICS 67.120
B51
National Standards of People's Republic of China
Determination of sea cucumber saponins in sea cucumber and its products
High performance liquid chromatography
Determination ofseacucumberglycosidesinseacucumberandsea
2016-10-13 released
2017-05-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Management Committee released
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed by the Ministry of Agriculture of the People's Republic of China.
This standard is under the jurisdiction of the National Aquatic Standardization Technical Committee (SAC/TC156).
This standard drafting unit. Qingdao City Product Quality Supervision and Inspection Research Institute, China Ocean University.
The main drafters of this standard. Xue Changhu, Li Zhaoyong, Fan Yan, Liu Xiaofang, Wu Qianjun, Yang Jie.
Determination of sea cucumber saponins in sea cucumber and its products
High performance liquid chromatography
1 Scope
This standard specifies the sea cucumber and its products in the sea cucumber saponins content of high performance liquid chromatography.
This standard applies to sea cucumber and its products in the determination of ginsenosides (quinolide) determination of quinolones for the sea cucumber saponin characteristic monosaccharides.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article
Pieces. For undated references, the latest edition (including all modifications) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
Methods of sampling aquatic products GB/T
3 terms and definitions
The following terms and definitions apply to this document.
3.1
Quinolose quinovose
6-Deoxy-D-gulcose 6-Deoxy-D-gulcose
Molecular formula C6H12O5, relative molecular mass 164.16.
Widely present in the sea cucumber saponin sugar chain, each of the sea cucumber saponin molecules and only contain a quinolone unit.
3.2
Seaweed saponins seacucumberglycosides
A triterpenoid saponin compound composed of a wool sterane derivative and an oligosaccharide, wherein the quinolose is a stable characteristic monosaccharide.
4 principle
The samples were extracted by ethanol and extracted with n-butanol to obtain sea cucumber saponins, hydrolyzed by trifluoroacetic acid, and the quinolones in the hydrolyzate were mixed with 1-phenyl-3-methyl
Yl-5-pyrazolone (PMP), the product was separated by C18 column, high performance liquid chromatography with UV detector, external standard
The content of ginseng saponins in the sample was indicated by quinolone.
5 reagents and materials
Unless otherwise stated, the reagents used are analytical reagents.
5.1 water is in line with GB/T 6682 provides a level of water.
5.2 Methanol (CH3OH). Chromatographic pure.
5.3 Acetonitrile (CH3CN). Chromatographic pure.
5.4 Ethanol (CH3CH2OH).
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