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GB/T 33035-2016: Detection and identification of Narcissus yellow stripe virus
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| GB/T 33035-2016 | English | 279 |
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Detection and identification of Narcissus yellow stripe virus
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GB/T 33035-2016
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Basic data | Standard ID | GB/T 33035-2016 (GB/T33035-2016) | | Description (Translated English) | Detection and identification of Narcissus yellow stripe virus | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020.01 | | Word Count Estimation | 14,199 | | Date of Issue | 2016-10-13 | | Date of Implementation | 2017-05-01 | | Regulation (derived from) | National Standard Notice No.1716 of 2016 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | GB/T 33035-2016: Detection and identification of Narcissus yellow stripe virus---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Narcissus yellow stripe virus
ICS 65.020.01
B16
National Standards of People's Republic of China
Identification method of narcissus yellow strip virus
2016-10-13 released
2017-05-01 implementation
General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China
China National Standardization Management Committee released
Foreword
This standard is drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The release of this document
The Agency does not assume responsibility for identifying these patents.
This standard is proposed by the National Commission for Standardization of Plant Quarantine Standardization (SAC/TC271).
The drafting unit of this standard. Fujian Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, China Inspection and Quarantine Science Research Institute, the Chinese People's Republic of China
And the Xiamen Entry-Exit Inspection and Quarantine Bureau, the People's Republic of China Ningbo Entry-Exit Inspection and Quarantine Bureau, Jiangsu Province, Hongze County Agricultural Bureau, Fujian Agriculture and Forestry
the University.
The main drafters of this standard. Shen Jianguo, Wei Meisheng, Liao Furong, Lin Shuangqing, Zhang Yongjiang, Jin Ling, Cai Wei, Guo Lixin, Gao Fangluan, Wu Zujian.
Identification method of narcissus yellow strip virus
1 Scope
This standard specifies the Narcissus yellowtail virus (Narcissusyellowstripevirus) quarantine identification method.
This standard is applicable to quarantine identification of narcissus and narcissus products that may carry Narcissus yellowes.
2 normative reference documents
The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article
Pieces. For undated references, the latest edition (including all modifications) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
SN/T 2122 Quarantine sampling of inbound and outbound plants and plant products
Code for the detection of plant viruses
Code for molecular biology detection of plant viruses
3 Narcissus yellow streak virus basic information
Chinese name. Narcissus yellow stripe virus.
Scientific name. Narcissusyellowstripevirus, abbreviation. NYSV.
Taxonomic status. Potato virus (Potyviridae), Potato virus (Potyvirus).
For additional information on Narcissus yellowes, see Appendix A.
4 Principle of the method
The serological and molecular biological characteristics of Narcissus yellowes are the main basis for the development of this quarantine identification method.
5 instruments, utensils and reagents
5.1 Instruments
High-speed refrigerated centrifuge, electronic balance, PCR instrument, fluorescence PCR instrument, horizontal electrophoresis system, -20 ℃ low temperature refrigerator and -80 ℃ ultra low
Temperature refrigerator, gel imaging analysis system, pH meter, microwave oven, magnetic stirrer, constant temperature water bath, autoclave, clean bench, ELISA
Instrument and so on.
5.2 Appliances
(2.5 μL, 10 μL, 20 μL, 100 μL,.200 μL, 1000 μL) and an adjustable pipette head, centrifuge tube,
Bowl and so on.
5.3 Reagents
Unless otherwise specified, all reagents are of analytical grade and the test water shall comply with the relevant provisions in GB/T 6682. PTA-ELISA assay
Reagents see Appendix B; RT-PCR detection reagents see Appendix C; real-time fluorescent RT-PCR detection reagents see Appendix D.
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