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GB/T 33019-2016 English PDF

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GB/T 33019-2016: Detection and identification of Pseudomonas syringae pv. persicae
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Basic data

Standard ID GB/T 33019-2016 (GB/T33019-2016)
Description (Translated English) Detection and identification of Pseudomonas syringae pv. persicae
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020.01
Word Count Estimation 14,165
Date of Issue 2016-10-13
Date of Implementation 2017-05-01
Regulation (derived from) National Standard Notice No.1716 of 2016
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 33019-2016: Detection and identification of Pseudomonas syringae pv. persicae

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Detection and identification of Pseudomonas syringae pv.persicae ICS 65.020.01 B16 National Standards of People's Republic of China Quarantine identification method of peach tree bacterial 2016-10-13 released 2017-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Management Committee released

Foreword

This standard is drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed by the National Commission for Standardization of Plant Quarantine Standardization (SAC/TC271). The drafting unit of this standard. Shenzhen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shenzhen Inspection and Quarantine Science Research Institute, the Chinese people Guangdong Province Entry-Exit Inspection and Quarantine Bureau, China Inspection and Quarantine Science Research Institute, Zhejiang Province Inspection and Quarantine Science and Technology Research Institute. The main drafters of this standard. Feng Jianjun, Li Qiufeng, Liu Xinjiao, Long Hai, Chen Dongmei, Zhao Wenjun, Feng Lixia, Zhang Guiming, Wu Ying. Quarantine identification method of peach tree bacterial

1 Scope

This standard specifies the method of quarantine identification of peach bacteria bacterial ulcer pathogens. This standard is applicable to the identification and identification of bacterial fungal pathogens in peach trees in international trade and pest monitoring.

2 normative reference documents

The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article Pieces. For undated references, the latest edition (including all modifications) applies to this document. GB 4789.28 National standards for food safety - Microbiological examination of food Quality requirements for media and reagents

3 peach bacteria bacterial ulcer bacteria basic information

Scientific name. Pseudomonassyringaepv.persicae (Prunier Luuisetti Synonyms. Pseudomonasorskruorumsubsp.persicae (Prunier Luuisetti Classification Status. Proteobacteria, Gammaproteobacteria, Pseudo- monadales, Pseudomonasaceae, Pseudomonas. Propagation. Streptomy breeding material is the main way of long-distance transmission, agricultural operation is the main way to spread and re-infection. See Appendix A for additional information on pathogens.

4 Principle of the method

The physiological characteristics, physiological and biochemical characteristics and pathogenicity of the pathogen, the characteristics of the symptoms and the physiological characteristics of the pathogen, And so on. 5 instruments, utensils and media 5.1 Instruments Clean bench, electronic balance (1/10000g), low temperature refrigerator, light incubator, automatic autoclave. 5.2 Appliances (0.2 mL, 1.5 mL, 15 mL), trace adjustable sampler (2 [mu] L, 10 [mu] L, (20 μL,.200 μL, 1000 μL), syringe and needle, pH meter, 20 μL, 100 μL,.200 μL, 5.3 medium Please refer to Appendix B for various media formulations and corresponding reagents.


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