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GB/T 17528-2009: Determination of pipeline content -- Method using HPLC
Status: Valid GB/T 17528: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| GB/T 17528-2009 | English | 139 |
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3 days [Need to translate]
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Determination of pipeline content -- Method using HPLC
| Valid |
GB/T 17528-2009
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| GB/T 17528-1998 | English | 239 |
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Determination of piperine content--Spectrophotometric method
| Obsolete |
GB/T 17528-1998
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PDF similar to GB/T 17528-2009
Basic data | Standard ID | GB/T 17528-2009 (GB/T17528-2009) | | Description (Translated English) | Determination of pipeline content -- Method using HPLC | | Sector / Industry | National Standard (Recommended) | | Classification of Chinese Standard | X14 | | Classification of International Standard | 67.200.10 | | Word Count Estimation | 6,688 | | Date of Issue | 2009-04-03 | | Date of Implementation | 2009-09-01 | | Older Standard (superseded by this standard) | GB/T 17528-1998 | | Adopted Standard | ISO 11027-1993, MOD | | Regulation (derived from) | National Standard Approval Announcement 2009 No.5 (Total No.145) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China | | Summary | This standard specifies the method for the determination of piperine content by HPLC. This standard applies to black, white pepper and black, white pepper and pepper oleoresin extraction alkali content of the product was measured. This standard method detection limit of 0. 008 g/100 g. | GB/T 17528-2009: Determination of pipeline content -- Method using HPLC---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of piperine content. Method using HPLC
ICS 67.200.10
X14
National Standards of People's Republic of China
Replacing GB/T 17528-1998
Pepper alkali content by HPLC
(ISO 11027.1993, Pepperandpepperoleoresins-Determination
chromatography, MOD)
Posted 2009-04-03
2009-09-01 implementation
Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China
Standardization Administration of China released
Foreword
This revised standard adopts ISO 11027.1993 "pepper and pepper oleoresin pepper alkali content by HPLC"
This standard and ISO 11027.1993 main differences are as follows.
--- The ISO 11027.1993 the name changed to Determinationofpiperinecontent-Methodusinghigh-per-
--- Increasing the detection limit;
--- The ISO 11027.1993 in a concentration range of 6.2 piperine standard solution "0.05g/L ~ 0.2g/L" changed to "0.4mg/L ~
2.0mg/L ";
--- The ISO 11027.1993 in 4.3 mobile phase "of acetonitrile and 1% acetic acid (48 + 52)" to "methanol - water (77 + 23)";
--- Cancellation ISO 11027.1993 10.1 10.2 formulas and calculations factor, because no formula to calculate the revised
factor.
This standard replaces GB/T 17528-1998 "Determination pepper alkali content spectrophotometry."
This standard and GB/T 17528-1998 compared to major differences are.
--- The spectrophotometric method to the High Performance Liquid Chromatography.
Appendix A of this standard is an informative annex.
The standard proposed by the People's Republic of China Ministry of Agriculture.
This standard by the national spice Standardization Technical Committee.
This standard was drafted. the Ministry of Agriculture Food Quality Supervision and Testing Center (Zhanjiang).
The main drafters of this standard. HEALTH LABORATORY Cheng Shenghua, Li Zhen Lin, Zhou Huiling, Lin Ling, Yu Bing search.
This standard was first released in October 1998.
Pepper alkali content by HPLC
1 Scope
This standard specifies the method for determining pepper alkali content by high performance liquid chromatography.
This standard applies to black, white and black pepper, white pepper and pepper oleoresin extraction product Content Determination.
The standard method detection limit of 0.008g/100g.
Principle 2
The sample was extracted with ethanol piperine detected by HPLC UV detector, external standard.
3 Reagents
Unless otherwise indicated in the analysis using only recognized as analytical grade reagents and distilled or deionized water or equivalent purity.
3.1 Ethanol (CH3CH2OH). 95% (mass fraction).
3.2 methanol (CH3OH). chromatography.
3.3 piperine standard substance. purity ≥98%.
Piperine 3.4 Standard solution. Accurately weigh (10 ± 0.1) mg piperine standard brown substance in 10mL beaker, ethanol (3.1) was dissolved
Solution, transferred to a 10mL brown volumetric flask with ethanol (3.1) to the mark, the concentration of the solution was 1000mg/L (when used with temporary
system).
3.5 piperine standard working solution. lessons 50μL piperine standard solution (3.4) with a micropipette placed in 25mL brown volumetric flask,
Ethanol (3.1) diluted to the mark, the concentration of the solution is 2.00mg/L.
4 Instrument
4.1 High performance liquid chromatograph. equipped with a UV detector.
4.2 Organization mashed machines.
4.3 Sample screens. a pore size of 500μm.
4.4 brown round bottom flask. 100mL, supporting reflux condenser apparatus.
4.5 brown volumetric flask. 10mL, 25mL, 100mL.
5 Sample Preparation
5.1 Whole pepper
With a stamp mill (4.2) the sample is crushed, until all the samples through the sieve (4.3), in a brown bottle storage spare.
5.2 pepper
All samples through the sample screen (4.3), not through a screen, with the masher (4.2) pulverized until the particle size to meet the requirements so far, in the storage
Brown bottle aside.
5.3 pepper oleoresin
The sample was sufficiently uniform.
Step 6 Analysis
Note. Because piperine solution is unstable, easily decomposed in light, dark operation is necessary. Operation with black paper or aluminum foil and the flask flask wrapped up, and
Determination as soon as possible.
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