GB/T 11446.10-2013 PDF English
US$120.00 · In stock · Download in 9 secondsGB/T 11446.10-2013: Test method for total bacterial count in electronic grade water by membrane filters Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedureStatus: Valid GB/T 11446.10: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| GB/T 11446.10-2013 | English | 120 |
Add to Cart
|
0-9 seconds. Auto-delivery
|
Test method for total bacterial count in electronic grade water by membrane filters
| Valid |
| GB/T 11446.10-1997 | English | 199 |
Add to Cart
|
2 days
|
Test method for total bacterial count in electronic grade water by membrane filters
| Obsolete |
| GB 11446.10-1989 | English | 199 |
Add to Cart
|
2 days
|
Test method for total bacterial count in electronic grade water by membrane filters
| Obsolete |
Excerpted PDFs (Download full copy in 9 seconds upon purchase)PDF Preview: GB/T 11446.10-2013
GB/T 11446.10-2013: Test method for total bacterial count in electronic grade water by membrane filters
---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GBT11446.10-2013
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 31-030
L 90
Replacing GB/T 11446.10-1997
Test method for total bacterial count in
electronic grade water by membrane filters
Issued on. DECEMBER 31, 2013
Implemented on. AUGUST 15, 2014
Issued by. General Administration of Quality Supervision, Inspection and
Quarantine;
Standardization Administration of the People's Republic of
China.
Table of Contents
Foreword... 3
1 Scope... 5
2 Normative references... 5
3 Terms and definitions... 5
4 Methodology... 6
5 Interference factors... 6
6 Reagents... 6
7 Measuring instruments and equipment... 7
8 Operation procedures... 8
9 Measurement result calculation... 8
10 Report... 9
Foreword
The estimated structure of GB/T 11446 is as follows.
- GB/T 11446.1 Electronic grade water;
- GB/T 11446.2 (To be determined);
- GB/T 11446.3 Generic rules for test methods of electronic grade water;
- GB/T 11446.4 Test methods for resistivity of electronic grade water;
- GB/T 11446.5 Test method for measuring trace metals in electronic grade
water by atomic absorption spectrophotometry;
- GB/T 11446.6 Test method for SiO2 in electronic grade water by
spectrophotometer;
- GB/T 11446.7 Test method for trace anion in electronic grade water by ion
chromatography;
- GB/T 11446.8 Test method for total organic carbon in electronic grade water;
- GB/T 11446.9 Test method for particles in electronic grade water by
instrument;
- GB/T 11446.10 Test method for total bacterial count in electronic grade
water by membrane filters.
This part is part 10 of GB/T 11446.
This part was drafted in accordance with the rules given in GB/T 1.1-2009.
This part replaces GB/T 11446.10-1997 “Test method for total bacterial count in
electronic grade water by membrane filters”.
As compared with GB/T 11446.10-1997, the main changes of this part are as
follows.
- In clause 3 “Terms and definitions”, REMOVE the definition of “sterile
operation”, ADD the definitions of “total bacteria” and “medium” (SEE
clause 3);
- ADD clause 5 “interference factors” (SEE clause 5);
- DELETE clause 10 “Considerations” (SEE clause 10 of the 1997 version).
This part was proposed by the Ministry of Industry and Information Technology
of the People's Republic of China.
This part shall be under the jurisdiction of China Electronics Standardization
Institute.
Drafting organizations of this part. Ministry of Information Industry Special
Materials Quality Supervision and Inspection Center, Chinese Academy of
Sciences Institute of Semiconductors, China Electronics Standardization
Institute, China Electronics Technology Group Corporation 46th Institute.
The main drafters of this part. Wang Yi, Qi Lianqing, He Xiukun, Duan
Shuguang, Ti Liuwang, Liu Jun.
This standard replaces the standard previously issued as follows.
- GB/T 11446.10-1989, GB/T 11446.10-1997.
Test method for total bacterial count in
electronic grade water by membrane filters
1 Scope
This part of GB/T 11446 specifies the test method for total bacterial count (live
bacteria) in electronic grade water by membrane filters.
This part applies to the determination of the total bacterial count in EW - I ~ EW
- IV electronic grade water.
2 Normative references
The following documents are essential to the application of this document. For
the dated documents, only the versions with the dates indicated are applicable
to this document; for the undated documents, only the latest version (including
all the amendments) are applicable to this standard.
GB/T 11446.1-2013 Electronic grade water
GB/T 11446.3-2013 Generic rules for test methods of electronic grade water
3 Terms and definitions
The terms and definitions defined in GB/T 11446.3-2013 and the following terms
and definitions apply to this document.
3.1
Bacterium colony
After a single cell or spore is cultured on a solid medium for a certain period
of time, it grows and multiplies to form a macroscopic microorganism colony,
which is a colony.
3.2
Bacterium culture method
A certain amount of water sample is filtered through an ultrafiltration
membrane, and bacteria remaining on the surface of the ultrafiltration
membrane are cultured with an appropriate medium to form colonies, which
are then counted to measure the amount of live bacteria in the water.
3.3
Total bacterial count
The total number of viable microorganisms present in a unit volume of
sample, excluding anaerobic organisms.
4 Methodology
Bacteria in water are trapped on the filter membrane through a 0.45 μm
microporous filter membrane, the membrane is attached to the substrate (filter
paper that has been fully absorbed with TGY medium), the culture is performed
under suitable conditions, and bacteria grow into macroscopic colonies.
5 Interference factors
5.1 The results of the analysis are affected by foreign microbial contamination,
which shall be given special attention when analyzing bacteria.
6 Reagents
6.1 Blank water. The EW-I electronic grade water specified in GB/T 11446.1-
2013 is sterilized in an autoclave at a pressure of 1.4 x 105 Pa for 15 minutes.
6.2 TGY medium. ADD 10.0 g of tryptone (BR), 6.0 g of beef extract (BR) and
2.0 g of glucose (AR) into 1 L of blank water, HEAT to dissolve it, COOL it to
room temperature, USE 1 mol/L sodium hydroxide to adjust the pH to 7.4 ~ 7.6,
6.3 1 mol/L sodium hydroxide solution. DISSOLVE 20 g of sodium hydroxide
(A.R.) in 500 mL of blank water and STORE it in a polyethylene bottle.
6.4 T.T.C solution 1.0 g/L is sterilized to prepare for use.
7 Measuring instruments and equipment
7.1 Sterile chamber (or sterile workbench). It is equipped with an ultraviolet
lamp with a power greater than or equal to 2W/m2 on the workbench at a
distance of 1.5 m from the workbench surface.
7.7 Filter. Φ50 mm cup type stainless steel filter.
7.8 All kinds of glassware. wide mouth bottles, flasks, petri dishes, measuring
cylinders, etc.
7.9 Alcohol lamp, flat tweezers, gauze, cotton, qualitative filter paper and so on.
7.10 Suction filtration system. as shown in Figure 1.
8 Operation procedures
8.1 Preparation before testing
8.1.1 TURN on the sterile chamber or sterile workbench for 1 h ~ 2 h, then
MAKE it into a stable working state.
8.2 Water sample determination
TAKE 2.0 mL of T.T.C solution, DROP it into 100 mL of medium at the
temperature of 46 °C ± 1 °C, MIX it uniformly in the Petri dish (about 3 mm ~ 4
mm thick), COOL it to prepare for use. SHAKE it uniformly, TAKE appropriate
amount of water sample.
9 Measurement result calculation
The total bacterial count in water is calculated in accordance with formula (1).
10 Report
Test reports shall be prepared in accordance with the format given in GB/T
11446.3-2013.
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
Tips & Frequently Asked QuestionsQuestion 1: How long will the true-PDF of English version of GB/T 11446.10-2013 be delivered?Answer: The full copy PDF of English version of GB/T 11446.10-2013 can be downloaded in 9 seconds, and it will also be emailed to you in 9 seconds (double mechanisms to ensure the delivery reliably), with PDF-invoice. Question 2: Can I share the purchased PDF of GB/T 11446.10-2013_English with my colleagues?Answer: Yes. The purchased PDF of GB/T 11446.10-2013_English will be deemed to be sold to your employer/organization who actually paid for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. www.ChineseStandard.us -- GB/T 11446.10-2013 -- Click this link and select your country/currency to pay, the exact amount in your currency will be printed on the invoice. Full PDF will also be downloaded/emailed in 9 seconds. Question 5: Should I purchase the latest version GB/T 11446.10-2013?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB/T 11446.10-2013 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically. How to buy and download a true PDF of English version of GB/T 11446.10-2013?A step-by-step guide to download PDF of GB/T 11446.10-2013_EnglishStep 1: Visit website https://www.ChineseStandard.net (Pay in USD), or https://www.ChineseStandard.us (Pay in any currencies such as Euro, KRW, JPY, AUD).
Step 2: Search keyword "GB/T 11446.10-2013".
Step 3: Click "Add to Cart". If multiple PDFs are required, repeat steps 2 and 3 to add up to 12 PDFs to cart.
Step 4: Select payment option (Via payment agents Stripe or PayPal).
Step 5: Customize Tax Invoice -- Fill up your email etc.
Step 6: Click "Checkout".
Step 7: Make payment by credit card, PayPal, Google Pay etc. After the payment is completed and in 9 seconds, you will receive 2 emails attached with the purchased PDFs and PDF-invoice, respectively.
Step 8: Optional -- Go to download PDF.
Step 9: Optional -- Click Open/Download PDF to download PDFs and invoice.
See screenshots for above steps: Steps 1~3 Steps 4~6 Step 7 Step 8 Step 9
|