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GB 6783-2013 English PDF

GB 6783-2013_English: PDF (GB6783-2013)
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GB 6783-2013English329 Add to Cart 3 days [Need to translate] Food additive -- Gelatin Valid GB 6783-2013


BASIC DATA
Standard ID GB 6783-2013 (GB6783-2013)
Description (Translated English) Food additive��Gelatine
Sector / Industry National Standard
Classification of Chinese Standard X41
Classification of International Standard 67.220.20
Word Count Estimation 14,161
Older Standard (superseded by this standard) GB 6783-1994
Drafting Organization National Food Industry Standardization Technical Committee
Administrative Organization National Health & Family Planning Commission of PRC
Regulation (derived from) China Food & Drug Administration [2013] No. 234, November, 1, 2013
Issuing agency(ies) National Health and Family Planning Commission of the People's Republic of China
Summary This Standard, from June 1, 2014, substitutes GB 6783-1994 Food additive-Gelatine. This standard applies to food additives: gelatin.

Standards related to: GB 6783-2013

GB 6783-2013
Food additive-Gelatine
National Standards of People's Republic of China
National Food Safety Standard
Food additive gelatin
Issued on. 2013-11-29
2014-06-01 implementation
Foreword
This standard replaces GB 6783-1994 "food additive gelatin."
This standard and GB 6783-1994 compared to the main changes are as follows.
- Modify the standard name;
- Remove the gelatin classification, unified index requirements gelatin;
- Removed Bertrand viscosity, pH, plasma point, the indicator of heavy metals;
- Modify transparency renamed transmittance, and revised the detection method;
- Added peroxide, lead indicator;
- Modify the arsenic, chromium, sulfur dioxide detection method;
- Removed the inspection rules, marking, packaging, transportation requirements.
National Food Safety Standard
Food additive gelatin
1 Scope
This standard applies to animal bone, skin, muscle, tendon and moderate scale and the like as raw materials by hydrolysis of the obtained food additive gelatin.
2 Requirements
2.1 Requirements for raw materials
2.1.1 can be used raw materials.
a) slaughterhouses, meat processing, canning factory, and other markets provided by the quarantine inspection of fresh cattle, pigs, sheep and fish and other animal skin, bone,
Muscle, tendon and scale and the like;
b) the inner layer of skin or skin with burrs under a cross-sectional front leather tanning process, under shear;
c) cleaning the bone grain processing factories bone particles and naturally dried aggregate.
2.1.2 prohibit use of raw materials.
a) Any waste tannery tanning after;
b) No inspection and quarantine certification of cattle, pigs, sheep and other animals or fish skin, bone, muscle, tendon and scale and the like;
c) was treated by the harmful use of organic solvents such as benzene or degreased animal skin, bone, muscle, tendon and scale and the like.
2.2 Sensory requirements
Sensory requirements should be consistent with Table 1.
Table 1 Sensory requirements
Project requires test methods
Light yellow to yellow color, take appropriate samples were placed in a clean transparent glass, natural
Light, color and observe its status as a solid state (such as grain, flakes, powder, etc.)
Odour Not odor formulated gelatin solution (2.5%), olfactory its flavor
2.3 Physical indicators
Physical and chemical requirements shall be in accordance with Table 2.
Table 2. Physical and chemical indicators
Item Index Test method Requirements
Water, w /% ≤ 14.0 GB 5009.3 a direct drying
Freezing intensity (6.67%)/(Bloom g) ≥ 50 Appendix A A.4
Ash, w /% ≤ 2.0 GB 5009.4 b
Transmittance /% Wavelength/nm
450 ≥ 30
Appendix A A.5
620 ≥ 50
TABLE 2 (cont.)
Project the indicator test methods
Water-insoluble, w /% ≤ 0.2 Appendix A A.6
Sulfur dioxide/(mg/kg) ≤ 30 Appendix A A.7
Peroxide/(mg/kg) ≤ 10 Appendix A A.8
Total arsenic (As)/(mg/kg) ≤ 1.0 GB/T 5009.11
Chromium (Cr)/(mg/kg) ≤ 2.0
GB/T 5009.123 graphite furnace atomic absorption method c
Or Appendix A, A.9
Lead (Pb)/(mg/kg) ≤ 1.5 GB 5009.12 Graphite Furnace Atomic Absorption Spectrometry
a sample weight of 1.0 g, accurate to 0.001 g, the drying temperature of 105 ℃ ± 2 ℃.
b sample weight of 1 g ± 0.1 g, accurate to 0.001g.
c arbitration law.
2.4 microbial indicators
Microbiological indicators comply with Table 3.
Table 3 microbiological indicators
Item Index Test method Requirements
Cfu/(CFU/g) ≤ 10000 GB 4789.2
Salmonella not detected GB 4789.4
Coliform/(MPN/g) ≤ 3 GB 4789.3 MPN counting
Appendix A
Testing method
A.1 General Provisions
Unless otherwise indicated in the analysis using only recognized as analytical reagents and GB/T 6682 set forth in the water. Analysis in the standard drop
Fixed solution, impurities measured by standard solution, preparations and products, did not indicate when the other requirements, according to GB/T 601, GB/T 602, GB/T
Preparation of the provisions of 603. This solution was used in the test does not indicate what is formulated with solvent, it refers to an aqueous solution.
A.2 preparation of the sample solution
Weigh a certain amount of the sample to the nearest 0.1 g, into a clean, dry container, according to the desired concentration by adding a certain amount of water in the room
Place at a temperature 2 h, sufficiently swelling, then the vessel was placed in a water bath at 65 ℃ ± 1 ℃, the slowly stirring within 20 min ± 5 min
Dissolution of a homogeneous liquid.
A.3 Identification Test
A.3.1 force in frozen vial formulation sample solution (6.67%) 120 mL, cooled to about 30 ℃, capped at 10 ℃ ± 0.1 ℃ low temperature tank
Cooling 16 h ~ 18 h, samples should be gelatinous, after heating to form a solution again.
A.3.2 Take a sample of about 0.5 g, water 50 mL, heated dissolved, take the solution 5 mL, add potassium dichromate solution (Weigh 7.5g dichromate
Potassium, formulated as a solution 100mL) and 4 parts of dilute hydrochloric acid (234mL amount of concentrated hydrochloric acid diluted to 1000mL) 1 part of a mixture of a few drops of orange that is generated
Yellow flocculent precipitate. Take the above identification test in the remaining solution 1 mL, water 100 mL, shake, add tannic acid test solution a few drops, which occurred
turbid.
Determination A.4 Freezing Strength
A.4.1 Principle
Under specified conditions, to a diameter of 12.7 mm inner cylinder is pressed into the surface of jelly containing 6.67% gelatin solution at below 4 mm,
Freezing strength of the applied force representatives to Bloom g units.
A.4.2 Instruments and Equipment
A.4.2.1 cold edge instrument. Organization analyzer or gel strength tester.
A.4.2.2 cylinder. Diameter 12.700 mm ± 0.013 mm.
A.4.2.3 cold edge Bottle. capacity 150 mL, an inner diameter of 59 mm, height 85 mm.
A.4.2.4 bath. You can control the temperature of 10 ℃ ± 0.1 ℃.
A.4.2.5 water bath. water bath to control the temperature of 65 ℃ ± 1 ℃.
A.4.2.6 flasks. 250 mL.
A.4.3 Analysis step
In the preparation of frozen force bottle 6.67% of the sample solution 120 mL, capped at 10 ℃ ± 0.1 ℃ cryogenic cooling tank 16 h ~ 18 h. The cold edge
Bottle was removed from the constant temperature water bath, quickly placed in cold force gauge round table. Cold edge instrument "depth" to select 4 mm, "speed" Select 0.5 mm/s or
1 mm/s, measured freezing strength, sample test will be completed within 2 min.
A.4.4 Calculation Results
Read directly from the power meter frozen Freezing measured intensity values, the unit represented in Bloom g. Results rounded, taking parallel determination results
The arithmetic mean of the measurement results. Two absolute difference between independent results under repeatability condition must not exceed 10 Bloom g.
A.5 Determination of transmittance
A.5.1 Principle
At 45 ℃, determined by spectrophotometry gelatin solution (6.67%) at a wavelength of 450 nm and 620 nm transmittance.
A.5.2 Instruments and Equipment
A.5.2.1 visible spectrophotometer.
A.5.2.2 balance. a sense of the amount of 0.1 g.
A.5.3 Analysis step
A.5.3.1 preparation of the sample solution (6.67%), and heated to 48 ℃.
A.5.3.2 spectrophotometer adjusted to a wavelength of 450 nm.
A.5.3.3 water as a reference to calibrate the instrument.
A.5.3.4 The solution was poured into 10 mm cuvette at 45 ℃, measured transmittance of the sample solution.
A.5.3.5 wavelength adjusted to 620 nm, and repeat A.5.3.3 ~ A.5.3.4 operation.
A.5.4 Calculation Results
Directly by percent transmittance two wavelengths (%) is represented. Results reserved bit integer. Take the parallel determination results as the arithmetic mean
The measurement results. The absolute difference between two independent results obtained under repeatability conditions should not exceed 1%.
A.6 Determination of insoluble matter
A.6.1 Principle
A glass filter crucible gelatin solution derived insolubles amount.
A.6.2 Instruments and Equipment
Sand core glass crucible. G3.
A.6.3 Analysis step
A.6.3.1 glass crucible at 105 ℃ ~ 110 ℃ drying, drying to a constant weight.
A.6.3.2 Weigh the sample 10 g ± 1 g, to the nearest 0.1 g, poured into a beaker, add 500 mL of water at room temperature for 2 h, then placed
Dissolved in a water bath at 65 ℃ ± 1 ℃, the dissolution time most grow in 0.5 h.
A.6.3.3 sample solution by suction filtration through a glass crucible.
A.6.3.4 glass crucible with hot water on the residue three times.
A.6.3.5 glass crucible placed 105 ℃ ~ 110 ℃ in the oven baking.
A.6.3.6 Remove the crucible from the oven, placed in a desiccator to cool to room temperature.
A.6.3.7 crucible weighed out.
A.6.3.8 A.6.3.5 ~ A.6.3.7 Repeat operation until constant weight.
A.6.4 Calculation Results
Sample water insoluble mass fraction w1 (%), according to formula (A.1) calculations.
1 
mm
w ... (A.1)
Where.
m0-- glass crucible mass in grams (g);
Quality m1-- glass crucible and residue, in grams (g);
m2-- sample mass, in grams (g).
Calculation result to two decimal places. Take the arithmetic mean of the parallel determination results of the measurement results. Obtained under repeatability conditions
Two independent determination results of absolute difference should not exceed 0.02%.
A.7 Determination of sulfur dioxide
A.7.1 Principle
Gelatin sulfite converted to sulfuric acid with a base titration, the content of sulfur dioxide is calculated by the amount of alkali consumed.
A.7.2 Reagents and materials
A.7.2.1 hydrogen peroxide solution. 3%.
A.7.2.2 bromophenol blue ethanol solution. 1 g/L. Bromophenol blue dissolved in the volume fraction of 20% ethanol.
A.7.2.3 sodium hydroxide standard titration solution. c (NaOH) = 0.01 mol/L.
A.7.2.4 hydrochloric acid solution. 2 mol/L.
A.7.3 Instruments and Equipment
Sulfur dioxide measurement device shown in Figure A.1.
A-- round-bottomed flask;
B-- separating funnel;
C-- condenser;
D-- tube;
E-- catheter.
Figure A.1 sulfur dioxide measurement device
A.7.4 Analysis step
A.7.4.1 added 150 mL of water in a flask (A), and the whole system with carbon dioxide gas (purity of not less than 99%), holding
Continued 15 min, a flow rate of 100 mL/min.
A.7.4.2 bromophenol blue was added hydrogen peroxide solution 10 mL - the ethanol solution of 0.15 mL, direct titration with sodium hydroxide standard titration solution
A blue to violet, can not drop too, and this solution was added to a test tube (D).
A.7.4.3 without prejudice to the case of removing the carbon dioxide stream separating funnel (B), the sample was added to the flask and 25.0g water 100 mL.
A.7.4.4 by a separatory funnel, the flask was added a solution of hydrochloric acid 80 mL, boiled for 1 h.
A.7.4.5 open piston separating funnel, carbon dioxide gas was stopped, and the heating was stopped and condensate.
A.7.4.6 small amount of water was added in a test tube, the inner tube and the solution was transferred to a 200 mL wide-mouth Erlenmeyer flask, and then from 60 ℃ ~
Heated 70 ℃ water bath 15 min, cooled.
A.7.4.7 added bromophenol blue - ethanol solution of 0.1 mL, and then titrated with standard sodium hydroxide solution, until the color changed from yellow to blue-violet
(Consumption volume V1).
A.7.4.8 once blank titration (consumption volume V0).
A.7.5 Calculation Results
Sample mass of sulfur dioxide content fraction w2, numerical order (mg/kg) according to formula (A.2) calculations.
  05.010060.64 01
2 
VVw ... (A.2)
Where.
Numerical 64.060-- sulfur dioxide (SO2) molar mass in grams per mole (g/mol) [M (SO2) = 64.060];
- Conversion factor;
0.5-- conversion factor;
V1 - consumption of sodium hydroxide standard titration solution volume in milliliters (mL);
V0 - Blank consume sodium hydroxide standard titration solution volume in milliliters (mL);
c - the concentration of sodium hydroxide standard titration solution, expressed in moles per liter (mol/L);
m - mass of the sample in grams (g).
The results rounded. Take the arithmetic mean of the parallel determination results of the measurement results. In two independent test repeated conditions
Given the absolute difference between the results should not exceed 1 mg/kg.
A.8 Determination of peroxide
A.8.1 Principle
Iodine Method, I use reduction assay oxidizing substances. In substance tested, if the presence of an oxidizing substance, is added over
Amount of potassium iodide and then titrated with sodium thiosulfate standard solution precipitated iodine.
A.8.2 Reagents and materials
A.8.2.1 sulfuric acid solution. 20%.
A.8.2.2 potassium iodide solution. 20 g/L.
A.8.2.3 starch solution. 10 g/L.
A.8.2.4 ammonium molybdate solution. 5 g/L.
A.8.2.5 sodium thiosulfate standard titration solution. 0.01 mol/L.
A.8.3 Analysis step
A.8.3.1 10 g sample was weighed in 250 mL conical flask, add 140 mL of water, swelling 2 h, dissolved as soon as possible in a water bath of 50 deg.] C,
Stirring gently to avoid bubbles, rapidly cooled to 30 ℃, and the following reagents were added sequentially.
a) sulfuric acid solution 6 mL, and shake;
b) potassium iodide solution 10 mL, and shake;
c) starch solution 2 mL ~ 3 mL, and shake;
d) ammonium molybdate solution 1 mL, shake thoroughly placed in the dark at 10 min.
A.8.3.2 standard titration with sodium thiosulfate solution titrated to a solution blue subsided, consumption of sodium thiosulfate standard titration solution volume V1.
A.8.3.3 according to A.8.3.1, A.8.3.2 do sample blank test.
A.8.4 Calculation Results
Sample peroxide mass fraction w3, with values (mg/kg) expressed by the formula (A.3) calculations.
 
5.01034 01
3 

cVV
w ...····· (A.3)
Where.
Numerical 34-- hydrogen peroxide (H2O2) molar mass in grams per mole (g/mol) [M (H2O2) = 34];
- Conversion factor;
0.5-- conversion factor;
Volume V1-- sample consumption of sodium thiosulfate standard titration solution, in milliliters (mL);
V0-- blank test volume consumption of sodium thiosulfate standard titration solution, in milliliters (mL);
c-- sodium thiosulfate standard titration solution concentration, in units of moles per liter (mol/L);
m-- sample mass, in grams (g).
The results rounded. Take the arithmetic mean of the parallel determination results of the measurement results. In two independent test repeated conditions
Given the absolute difference between the results should not exceed 1 mg/kg.
A.9 chromium (Cr) Determination
A.9.1 Principle
Using diphenyl hydrazine (diphenyl semicarbazide) colorimetry. Diphenyl hydrazine (diphenylamino urea) to form a red complex with chromium,
Absorbance was measured at 540 nm, the absorbance value of the concentration of chromium in line with Lang Bo - Beer law.
A.9.2 Instruments and Equipment
A.9.2.1 721 type visible spectrophotometer or equivalent required to achieve visible spectrophotometer.
A.9.2.2 high-temperature furnace.
A.9.3 Reagents and materials
A.9.3.1 potassium permanganate solution (5 g/L). Weigh 0.5 g of potassium permanganate, dissolved and diluted to 100 mL, stored in a brown bottle.
A.9.3.2 urea solution (100 g/L). Weigh 10 g of urea, dissolved and diluted to 100 mL, stored in a brown bottle, placed in a dark cold
Place.
A.9.3.3 sodium nitrite solution (100 g/L). Weigh 10 g of sodium nitrite, dissolved and diluted to 100 mL, stored in a brown bottle,
Placed in the dark, or using now.
Acetone solution of hydrazine A.9.3.4 Diphenylcarbazide. Weigh 0.125g diphenyl hydrazine, dissolved in a 25 mL of acetone and 25 mL of water dubbed
Mixture, using now, placed in the dark.
A.9.3.5 sodium pyrophosphate solution (5 g/L). Weigh 0.5 g of sodium pyrophosphate, dissolved and diluted to 100 mL, stored in a reagent bottle.
A.9.3.6 chromium standard solution (0.02 mg/mL). Weigh accurately 0.0566 g potassium dichromate (excellent pure, finely ground in an agate mortar,
And, after 105 ℃ ~ 110 ℃ dried 3 h ~ 4 h, placed in the dryer to cool), placed in a small beaker, dissolved in water and transferred to 100 mL capacity
Flask, add water to the mark.
A.9.3.7 sulfuric acid solution (1 mol/L). Measure 28 mL of sulfuric acid, was slowly added a certain amount of pure water containing 500 mL volumetric flask, and then
Add water to the mark.
A.9.4 Analysis step
A.9.4.1 Drawing standard curve. Pipette chromium standard solution 0.00 mL, 0.20 mL, 0.40 mL, 0.60 mL, 1.00 mL, 1.60
mL, 2.00 mL, 2.60 mL, equivalent chromium 0.0 μg, 4.0 μg, 8.0 μg, 12.0 μg, 20.0 μg, 32.0 μg, 40.0 μg, 52.0
μg, were placed in a beaker. Sulfuric acid solution added to each 10 mL and 10 mL of water, heated to boiling, dropping potassium permanganate solution to the solution does not fade
Color, cooling, quantitatively transferred to a 50 mL volumetric flask, add urea solution 10 mL, shake vigorously drop Gaja nitrate solution to a solution
fade. Sodium pyrophosphate solution was added 2.0 mL, diphenyl hydrazine 0.5 mL. Volume with water, shake, place 30 min after the wavelength
Absorbance at 540 nm was measured, plotted ccr-D540 standard curve.
A.9.4.2 Sample pretreatment and determination. Accurately weigh 1.000 g gelatin in a crucible, slowly warming to make charring, let cool. Add concentrated nitric
A few drops of acid, slowly heated, the gas evolution had ceased, moved into a high temperature oven at 600 ℃ for burning until all black particles disappear (2 h),
After removing the sulfuric acid solution to be cooled 10 mL and 20 mL of water to dissolve the residue, heated on a water bath for 5 min. Solution of potassium permanganate solution to cook
Boiling, and then dropping the solution of potassium permanganate solution boiled purple disappear, and so forth until the purple does not fade so far, let cool, add the urea solution
10 mL, shake vigorously dripping Gaja nitrate solution, until the complete elimination of excess potassium permanganate solution colorless. Such as manganese dioxide Ming
Memory in the filter. Quantitatively transferred to a 50 mL volumetric flask, add sodium pyrophosphate solution, 2 mL, diphenyl hydrazine solution 0.5 mL,
Volume with water, shake. Place 30 min.
A.9.4.3 Take the resulting sample solution A.9.4.2, measured absorbance at 540 nm wavelength, can be curved by the standard work ccr-D540
Line chromium content in the sample is calculated.
A.9.5 Calculation Results
The ccr-D540 standard curve calculated from the chromium content in milligrams per kilogram (mg/kg). Results are expressed to one decimal place.
Take the arithmetic mean of the parallel determination results of the measurement results. Two independent determination results in the absolute difference obtained under repeatability conditions should not be
Chaoguo 0.2 mg/kg.
...