GB 5009.154-2023 PDF English
US$380.00 · In stock · Download in 9 secondsGB 5009.154-2023: National food safety standard - Determination of vitamin B6 in foods Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedureStatus: Valid GB 5009.154: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| GB 5009.154-2023 | English | 380 |
Add to Cart
|
0-9 seconds. Auto-delivery
|
National food safety standard - Determination of vitamin B6 in foods
| Valid |
| GB 5009.154-2016 | English | 115 |
Add to Cart
|
0-9 seconds. Auto-delivery
|
National food safety standard -- Determination of vitamin B6 in foods
| Obsolete |
| GB/T 5009.154-2003 | English | 199 |
Add to Cart
|
2 days
|
Determination of vitamin B6 in foods
| Obsolete |
Excerpted PDFs (Download full copy in 9 seconds upon purchase)PDF Preview: GB 5009.154-2023
GB 5009.154-2023: National food safety standard - Determination of vitamin B6 in foods---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB5009.154-2023
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
National Food Safety Standard - Determination of Vitamin
B6 in Foods
Issued on. SEPTEMBER 6, 2023
Implemented on. MARCH 6, 2024
Issued by. National Health Commission of the People’s Republic of China;
State Administration for Market Regulation.
Table of Contents
Foreword... 4
1 Scope... 5
Method I - Liquid Chromatography - Tandem Mass Spectrometry... 5
2 Principle... 5
3 Reagents and Materials... 5
4 Instruments and Equipment... 8
5 Analytical Procedures... 9
6 Expression of Analysis Results... 13
7 Precision... 14
8 Others... 14
Method II - Liquid Chromatography - Mass Spectrometry... 14
9 Principle... 14
10 Reagents and Materials... 14
11 Instruments and Equipment... 17
12 Analytical Procedures... 18
13 Expression of Analysis Results... 21
14 Precision... 22
15 Others... 22
Method III - High-performance Liquid Chromatography - Fluorescence Detection
Method... 22
16 Principle... 22
17 Reagents and Materials... 22
18 Instruments and Equipment... 24
19 Analytical Procedures... 25
20 Expression of Analysis Results... 26
21 Precision... 27
22 Others... 27
Method IV - Microbiological Method... 27
1 Scope
This Standard specifies the method for the determination of vitamin B6 in foods.
The first method “liquid chromatography - tandem mass spectrometry” is applicable to the
determination of vitamin B6 in foods.
The second method “liquid chromatography - mass spectrometry” is applicable to the
determination of vitamin B6 (pyridoxamine, pyridoxal and pyridoxine) in formulated milk
powder, special dietary foods, ready-to-eat cereals, baked goods and beverages, in which,
vitamin B6 (pyridoxamine, pyridoxal and pyridoxine) is added as a nutritional fortifier.
The third method “high-performance liquid chromatography - fluorescence detection method”
is applicable to the determination of vitamin B6 (pyridoxamine, pyridoxal and pyridoxine) in
formulated milk powder, special dietary foods (except foods for special medical purposes),
ready-to-eat cereals, baked goods and beverages, in which, vitamin B6 (pyridoxamine,
pyridoxal and pyridoxine) is added as a nutritional fortifier.
The fourth method “microbiological method” is applicable to the determination of vitamin B6
in foods.
Method I - Liquid Chromatography - Tandem Mass
Spectrometry
2 Principle
Vitamin B6 (pyridoxamine, pyridoxal and pyridoxine) in foods is firstly hydrolyzed by acid,
then, enzymatically hydrolyzed into pyridoxamine, pyridoxal and pyridoxine. After dilution
and filtration, reversed-phase liquid chromatography separation, tandem mass spectrometry
detection, and isotope internal standard method for quantitative determination, the total vitamin
B6 content is calculated in terms of pyridoxine.
3 Reagents and Materials
Unless it is otherwise specified, the reagents used in this Method are all analytically pure, and
the water is Grade-1 water specified in GB/T 6682.
3.1 Reagents
3.2 Reagent Preparation
3.3 Reference Materials
3.4 Preparation of Standard Solutions
3.5 Preparation of Isotope Internal Standard Solutions
4 Instruments and Equipment
4.1 Liquid chromatography - tandem mass spectrometer. equipped with electrospray ion source.
4.3 Constant-temperature incubator, or one with equivalent performance.
4.4 pH meter. with an accuracy of 0.01.
4.5 Vortex mixer.
4.6 Spectrophotometer.
4.7 High-speed pulverizer.
4.8 Homogenizer.
4.9 Constant-temperature water bath or autoclave.
5 Analytical Procedures
5.1 Specimen Preparation
For meat, vegetables and fruits, etc., take the edible parts, use water to wash them, and use dry
gauze to wipe off the surface moisture, use a homogenizer to homogenize them and store in
sample bottles for later use. For powdery samples (milk powder and rice noodles, etc.), evenly
mix them and conduct direct sampling. For liquid samples, evenly shake them and reserve them
for later use. For liquid samples with granules, for example, large-grained yogurt, use a
homogenizer to homogenize them and reserve them for later use. For flaky and granular
samples, use a high-speed pulverizer to grind them into powder and seal for later use.
5.2 Specimen Treatment
5.3 Determination Conditions of Instruments
5.4 Drawing of Standard Curve
Inject the vitamin B6 mixed standard series working solutions into the liquid chromatography -
mass spectrometer from low to high concentrations. Take the concentrations of the working
solutions of pyridoxine, pyridoxal and pyridoxamine as the x-coordinate, and the peak area
ratio of pyridoxine, pyridoxal and pyridoxamine to their corresponding isotope internal
standards as the y-coordinate. Respectively draw the standard curves of pyridoxamine,
pyridoxal and pyridoxine.
5.5 Determination of Specimen Solution
Inject the solution to be tested. In accordance with the three standard curves drawn above, adopt
the internal standard method to calculate the concentration of pyridoxine, pyridoxal and
pyridoxamine in the specimen solution to be tested.
6 Expression of Analysis Results
6.1 The content of each component of vitamin B6 in the specimen is calculated in accordance
with Formula (1).
6.2 Total content of vitamin B6 (calculated as pyridoxine)
The total content of vitamin B6 in the specimen is calculated in accordance with Formula (2).
7 Precision
The absolute difference between the results of two independent determinations obtained under
repeatability conditions shall not exceed 10% of the arithmetic mean.
8 Others
8.1 Solid specimens. when the sampling size is 1.00 g, the detection limit of the Method is. 0.03
mg/kg for pyridoxine, 0.03 mg/kg for pyridoxal and 0.03 mg/kg for pyridoxamine; the
quantitation limit is 0.10 mg/kg for pyridoxine, 0.10 mg/kg for pyridoxal and 0.10 mg/kg for
pyridoxamine.
8.2 Liquid specimens. when the sampling size is 5.00 g, the detection limit of the Method is.
0.01 mg/kg for pyridoxine, 0.01 mg/kg for pyridoxal and 0.01 mg/kg for pyridoxamine;
9 Principle
Vitamin B6 (pyridoxine, pyridoxal and pyridoxamine) added to foods is subject to extraction,
dilution and filtration, and separation by reversed-phase liquid chromatographic column,
nitrogen and single quadrupole mass spectrometry detection. Adopt the isotope dilution internal
standard method to respectively quantify pyridoxamine, pyridoxal and pyridoxine. The total
vitamin B6 content is calculated in terms of pyridoxine.
10 Reagents and Materials
Unless it is otherwise specified, the reagents used in this Method are all analytically pure, and
the water is Grade-1 water specified in GB/T 6682.
10.1 Reagents
10.1.1 Methanol (CH3OH). chromatographically pure.
10.1.2 Formic acid (HCOOH). chromatographically pure.
10.1.3 Ammonium formate (HCOONH4). chromatographically pure.
10.1.4 Hydrochloric acid (HCl).
10.1.5 Sodium hydroxide (NaOH).
10.2 Reagent Preparation
10.2.1 Hydrochloric acid solution (0.1 mol/L). accurately draw 9 mL of hydrochloric acid and
use water to dilute to 1,000 mL.
10.2.2 Sodium hydroxide solution (0.1 mol/L). accurately weigh-take 0.4 g of sodium
hydroxide, add 50 mL of water to dissolve it; after cooling, use water to dilute to 100 mL.
10.3 Reference Materials
10.4 Preparation of Standard Solutions
10.4.5 Vitamin B6 standard mixed working solution (5.00 g/mL). accurately draw 1.00 mL of
vitamin B6 standard mixed stock solution (50.0 g/mL) into a 10 mL brown volumetric flask,
use mobile phase A to dilute to the scale. Prepare it right before use.
10.5 Preparation of Isotope Internal Standard Solutions
10.6 Preparation of Standard Series of Working Solutions
Respectively and accurately draw an appropriate amount of vitamin B6 standard mixed working
solution into a 50 mL brown volumetric flask, and accurately add 100 L of vitamin B6 isotope
internal standard mixed working solution (50.0 g/mL). Use mobile phase A to dilute to the
scale. The mass concentration of the vitamin B6 (pyridoxamine, pyridoxal and pyridoxine)
standard series working solutions is respectively 10.0 ng/mL, 20.0 ng/mL, 40.0 ng/mL, 100
ng/mL, 200 ng/mL and 250 ng/mL. Their corresponding isotope internal standard
concentrations are all 100 ng/mL. Prepare them right before use.
11 Instruments and Equipment
11.1 Liquid chromatography - mass spectrometer. equipped with electrospray ion source.
11.2 Balance. with a division value of 0.01 g and 0.1 mg.
11.3 Constant-temperature incubator, or one with equivalent performance.
11.4 pH meter. with an accuracy of 0.01.
11.8 Homogenizer.
11.9 Constant-temperature water bath.
12 Analytical Procedures
12.2 Specimen Treatment
12.3 Determination Conditions of Instruments
12.3.1 Reference conditions of chromatography
The reference conditions of chromatography are as follows.
12.3.2 Reference conditions of mass spectrometry
The reference conditions of mass spectrometry are as follows.
12.4 Drawing of Standard Curve
Inject the vitamin B6 (pyridoxine, pyridoxal and pyridoxamine) mixed standard series working
solutions into the liquid chromatography - mass spectrometer from low to high concentrations.
Take the concentrations of the working solutions of pyridoxine, pyridoxal and pyridoxamine as
the x-coordinate, and the peak area ratio of pyridoxine, pyridoxal and pyridoxamine to their
corresponding isotope internal standards as the y-coordinate. Respectively draw the standard
curves of pyridoxamine, pyridoxal and pyridoxine.
12.5 Determination of Specimen Solution
Inject the solution to be tested. In accordance with the three standard curves drawn above, adopt
the internal standard method to calculate the concentration of pyridoxine, pyridoxal and
pyridoxamine in the specimen solution to be tested.
12.6 Qualitative Determination
When comparing the retention time of the chromatographic peak of the target compound in the
specimen with the retention time of the corresponding standard chromatographic peak,
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
Tips & Frequently Asked QuestionsQuestion 1: How long will the true-PDF of English version of GB 5009.154-2023 be delivered?Answer: The full copy PDF of English version of GB 5009.154-2023 can be downloaded in 9 seconds, and it will also be emailed to you in 9 seconds (double mechanisms to ensure the delivery reliably), with PDF-invoice. Question 2: Can I share the purchased PDF of GB 5009.154-2023_English with my colleagues?Answer: Yes. The purchased PDF of GB 5009.154-2023_English will be deemed to be sold to your employer/organization who actually paid for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. www.ChineseStandard.us -- GB 5009.154-2023 -- Click this link and select your country/currency to pay, the exact amount in your currency will be printed on the invoice. Full PDF will also be downloaded/emailed in 9 seconds. Question 5: Should I purchase the latest version GB 5009.154-2023?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB 5009.154-2023 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically. How to buy and download a true PDF of English version of GB 5009.154-2023?A step-by-step guide to download PDF of GB 5009.154-2023_EnglishStep 1: Visit website https://www.ChineseStandard.net (Pay in USD), or https://www.ChineseStandard.us (Pay in any currencies such as Euro, KRW, JPY, AUD).
Step 2: Search keyword "GB 5009.154-2023".
Step 3: Click "Add to Cart". If multiple PDFs are required, repeat steps 2 and 3 to add up to 12 PDFs to cart.
Step 4: Select payment option (Via payment agents Stripe or PayPal).
Step 5: Customize Tax Invoice -- Fill up your email etc.
Step 6: Click "Checkout".
Step 7: Make payment by credit card, PayPal, Google Pay etc. After the payment is completed and in 9 seconds, you will receive 2 emails attached with the purchased PDFs and PDF-invoice, respectively.
Step 8: Optional -- Go to download PDF.
Step 9: Optional -- Click Open/Download PDF to download PDFs and invoice.
See screenshots for above steps: Steps 1~3 Steps 4~6 Step 7 Step 8 Step 9
|