GB 5009.137-2025 PDF English
US$110.00 · In stock · Download in 9 secondsGB 5009.137-2025: National food safety standard - Determination of antimony in foods Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedureStatus: Valid GB 5009.137: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| GB 5009.137-2025 | English | 110 |
Add to Cart
|
0-9 seconds. Auto-delivery
|
National food safety standard - Determination of antimony in foods
| Valid |
| GB 5009.137-2016 | English | 80 |
Add to Cart
|
0-9 seconds. Auto-delivery
|
National food safety standard - Determination of Antimony in Foods
| Valid |
| GB/T 5009.137-2003 | English | 239 |
Add to Cart
|
3 days
|
Determination of antimony in foods
| Obsolete |
Excerpted PDFs (Download full copy in 9 seconds upon purchase)PDF Preview: GB 5009.137-2025
GB 5009.137-2025: National food safety standard - Determination of antimony in foods---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB5009.137-2025
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
National Food Safety Standard - Determination of Antimony
in Foods
Issued on: MARCH 16, 2025
Implemented on: SEPTEMBER 16, 2025
Issued by. National Health Commission of the People’s Republic of China;
State Administration for Market Regulation.
Table of Contents
Foreword... 3
1 Scope... 4
Method I - Hydride Atomic Fluorescence Spectrometry... 4
2 Principle... 4
3 Reagents and Materials... 4
4 Instruments and Equipment... 6
5 Analytical Steps... 7
6 Expression of Analytical Results... 9
7 Precision... 10
8 Others... 10
Method II - Inductively Coupled Plasma Mass Spectrometry... 10
Appendix A Reference Conditions of Microwave Digestion Instrument... 11
National Food Safety Standard - Determination of Antimony
in Foods
1 Scope
This Standard specifies the determination method of antimony in foods by hydride atomic
fluorescence spectrometry and inductively coupled plasma mass spectrometry.
This Standard is applicable to the determination of antimony in foods.
Method I - Hydride Atomic Fluorescence Spectrometry
2 Principle
After the specimen is digested by acid heating, in an acidic medium, the antimony in the
specimen reacts with sodium borohydride or potassium borohydride to generate volatile
antimony hydride, which is carried into the atomizer by the carrier gas for atomization. Under
the excitation of the antimony hollow cathode lamp, atomic fluorescence is generated, and its
fluorescence intensity is proportional to the antimony content and is quantified by the external
standard method.
3 Reagents and Materials
Unless otherwise specified, all reagents used in this Method are guaranteed reagents and the
water is Grade-2 water specified in GB/T 6682.
3.1 Reagents
3.1.1 Nitric acid (HNO3).
3.1.2 Hydrochloric acid (HCl).
3.1.3 Perchloric acid (HClO4).
3.1.4 Thiourea [(NH2)2CS]. analytical grade.
3.1.5 Potassium iodide (KI). analytical grade.
3.1.6 Ascorbic acid (C6H8O6). analytical grade.
3.1.7 Potassium borohydride (KBH4) or sodium borohydride (NaBH4).
3.1.8 Potassium hydroxide (KOH) or sodium hydroxide (NaOH).
3.1.9 Tartaric acid (C4H6O6). purity 99.5%.
3.1.10 Argon (Ar). purity > 99.99%.
3.2 Preparation of Reagents
3.2.1 Nitric acid-perchloric acid mixture (10 + 1). measure-take 10 mL of perchloric acid and
slowly add it into 100 mL of nitric acid and mix it well.
3.2.2 Hydrochloric acid solution (1 + 1). measure-take 50 mL of hydrochloric acid, add it to 50
mL of water and mix it well.
3.2.3 Thiourea-ascorbic acid solution. respectively weigh-take 10.0 g of thiourea and 10.0 g of
ascorbic acid, dissolve in 100 mL of water, mix it well, and store it in a dark place. Prepare it
immediately before use.
3.2.4 Thiourea-potassium iodide solution. respectively weigh-take 2.0 g of thiourea and 10.0 g
of potassium iodide, dissolve in 100 mL of water, mix it well, and store it in a dark place.
Prepare it immediately before use.
3.2.5 Potassium hydroxide solution (5.0 g/L). weigh-take 5.0 g of potassium hydroxide, use
water to dissolve it and dilute to 1,000 mL, and mix it well. Prepare it immediately before use.
The potassium hydroxide in the solution may also be replaced by sodium hydroxide.
3.2.6 Potassium borohydride solution (20 g/L). weigh-take 10.0 g of potassium borohydride,
use potassium hydroxide solution (5.0 g/L) to dissolve it and dilute it to 500 mL, mix it well,
and prepare it immediately before use. The potassium borohydride in the solution may also be
replaced by 7.0 g of sodium borohydride.
3.2.7 Nitric acid solution (1 + 5). measure-take 100 mL of nitric acid, add it to 500 mL of water
and mix it well.
3.2.8 Hydrochloric acid solution (5 + 95). measure-take 50 mL of hydrochloric acid, add it to
950 mL of water, and mix it well.
3.2.9 Nitric acid solution (1 + 1). measure-take 50 mL of nitric acid, add it to 50 mL of water,
and mix it well.
3.2.10 Nitric acid solution (2.5 mol/L). measure-take 173 mL of nitric acid, use water to
dissolve it and dilute it to 1,000 mL, and mix it well.
3.3 Standard Substance
Metallic antimony (Sb, CAS No.. 7440-36-0). purity > 99.99%.
3.4 Preparation of Standard Solutions
3.4.1 Antimony standard stock solution (1,000 mg/L). weigh-take 0.50 g (accurate to 0.0001 g)
of metallic antimony into a beaker, add 5.0 g of tartaric acid and 10.0 mL of nitric acid solution
(1 + 1), after dissolution, transfer it to a 500 mL volumetric flask, use 2.5 mol/L nitric acid
solution to reach a constant volume to the scale and mix it well. Place in a polyethylene plastic
bottle, and store at room temperature away from light. The shelf life is 2 years. Or use antimony
standard solution that has been certified by the state and awarded a standard substance
certificate.
3.4.2 Antimony standard intermediate solution (100 mg/L). accurately pipette 1.00 mL of
antimony standard stock solution (1,000 mg/L) into a 10 mL volumetric flask, add water to the
scale and mix it well. Place it in a polyethylene plastic bottle, and store at 0 C ~ 5 C in a
sealed container away from light. The shelf life is 6 months.
3.4.3 Antimony standard working solution (1.00 mg/L). accurately pipette 1.00 mL of antimony
standard intermediate solution (100 mg/L) into a 100 mL volumetric flask, add water to the
scale and mix it well. Place it in a polyethylene plastic bottle, and store at 0 C ~ 5 C in a
sealed container away from light. The shelf life is 1 month.
3.4.4 Antimony standard series solutions. respectively and accurately pipette 0 mL, 0.100 mL,
0.200 mL, 0.400 mL, 0.600 mL, 0.800 mL, 1.00 mL and 2.00 mL of antimony standard working
solution (1.00 mg/L) into 100 mL volumetric flasks, add a small amount of water to dilute them,
then, add 10 mL of hydrochloric acid solution (1 + 1), 10 mL of thiourea-potassium iodide
solution or thiourea-ascorbic acid solution, add water to reach a constant volume to the scale
and mix them well. The mass concentration of the antimony standard series solutions is 0 g/L,
1.00 g/L, 2.00 g/L, 4.00 g/L, 6.00 g/L, 8.00 g/L, 10.0 g/L and 20.0 g/L. After leaving
them for 1 hour, conduct the determination. Prepare them immediately before use.
NOTE. the mass concentration range of antimony in the standard series solutions can be determined
based on the sensitivity of the instrument, the actual antimony content in the sample and
different instrument models.
4 Instruments and Equipment
NOTE. all glassware and the polytetrafluoroethylene digestion inner tank and inner cover must be
soaked in nitric acid solution (1 + 5) overnight, rinsed repeatedly with tap water, and finally
rinsed with water.
4.1 Atomic fluorescence spectrometer. equipped with antimony hollow cathode lamp.
4.2 Balance. the division value is respectively 1 mg and 0.1 mg.
4.3 Adjustable electric heating plate.
4.4 Adjustable electric furnace.
4.5 Microwave digestion system. equipped with polytetrafluoroethylene digestion tank.
solution is colorless, transparent or slightly yellow, continue heating to drive out the acid, until
the volume reaches 0.5 mL ~ 1 mL. After cooling, transfer the solution to a 25 mL container
and use a small amount of water to rinse the container several times. After all the solution has
been transferred, add 2.5 mL of hydrochloric acid solution (1 + 1), add 2.5 mL of thiourea-
potassium iodide solution or thiourea-ascorbic acid solution, use water to dilute it and reach a
constant volume of 25 mL, shake it well, and after leaving it for 1 h, conduct the determination.
Meanwhile, perform a blank test.
5.2.2 Microwave digestion
Weigh-take 0.2 g ~ 0.8 g (accurate to 0.001 g; for the high-water content samples, the sampling
amount can be appropriately increased to 1 g) of solid sample or pipette 1.00 mL ~ 3.00 mL of
liquid specimen and place it in a microwave digestion tank. For samples containing ethanol or
carbon dioxide, first, heat on an electric heating plate at low temperature to remove the ethanol
or carbon dioxide, add 5 mL ~ 7 mL of nitric acid. In accordance with the operating procedures
of microwave digestion (see Appendix A for the reference conditions of digestion), digest the
specimen. After digestion is completed, wait for the digestion tank to cool down and then, open
it, heat it at 140 C ~ 160 C to drive out the acid, until the volume reaches 0.5 mL ~ 1 mL, use
a small amount of water to rinse the digestion tank several times, combine the washing liquid
in a 25 mL container, add 2.5mL of hydrochloric acid solution (1 + 1); add 2.5 mL of thiourea-
potassium iodide solution or thiourea-ascorbic acid solution, use water to dilute it and reach a
constant volume of 25 mL, shake it well, and after leaving it for 1 h, conduct the determination.
Meanwhile, perform a blank test.
5.2.3 Pressure tank digestion
Weigh-take 0.2 g ~ 1 g of solid specimen (accurate to 0.001 g; for the high-water content
samples, the sampling amount can be appropriately increased to 2 g) or pipette 1.00 mL ~ 5.00
mL of liquid specimen and place it in a polytetrafluoroethylene inner tank. For samples
containing ethanol or carbon dioxide, first, heat on an electric heating plate at low temperature
to remove the ethanol or carbon dioxide, and then, add 4 mL ~ 8 mL of nitric acid to soak
overnight. Put on the inner cover, tighten the stainless-steel sheath, put it into the constant-
temperature drying oven, and maintain it at 140 C ~ 160 C for 4 h ~ 5 h, in the oven, naturally
cool to room temperature. Open the cover and take out the inner tank, heat at 140 C ~ 160 C
to drive out the acid, until the volume reaches 0.5 mL ~ 1 mL. Use a small amount of water to
rinse the digestion tank several times, combine the washing liquid in a 25 mL container, add
2.5 mL of hydrochloric acid solution (1 + 1), add 2.5 mL of thiourea-potassium iodide solution
or thiourea-ascorbic acid solution, use water to dilute it and reach a constant volume of 25 mL,
and shake it well. After leaving it for 1 h, conduct the determination. Meanwhile, perform a
blank test.
5.3 Instrument Reference Conditions
Instrument reference conditions. photomultiplier tube voltage is 270 V; hollow cathode lamp
current is 40 mA; atomizer height is 8 mm; carrier gas is argon; carrier gas flow rate is 400
mL/min; shield gas flow rate is 800 mL/min; measurement mode is standard curve method;
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
Tips & Frequently Asked QuestionsQuestion 1: How long will the true-PDF of English version of GB 5009.137-2025 be delivered?Answer: The full copy PDF of English version of GB 5009.137-2025 can be downloaded in 9 seconds, and it will also be emailed to you in 9 seconds (double mechanisms to ensure the delivery reliably), with PDF-invoice. Question 2: Can I share the purchased PDF of GB 5009.137-2025_English with my colleagues?Answer: Yes. The purchased PDF of GB 5009.137-2025_English will be deemed to be sold to your employer/organization who actually paid for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. www.ChineseStandard.us -- GB 5009.137-2025 -- Click this link and select your country/currency to pay, the exact amount in your currency will be printed on the invoice. Full PDF will also be downloaded/emailed in 9 seconds. Question 5: Should I purchase the latest version GB 5009.137-2025?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB 5009.137-2025 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically. How to buy and download a true PDF of English version of GB 5009.137-2025?A step-by-step guide to download PDF of GB 5009.137-2025_EnglishStep 1: Visit website https://www.ChineseStandard.net (Pay in USD), or https://www.ChineseStandard.us (Pay in any currencies such as Euro, KRW, JPY, AUD).
Step 2: Search keyword "GB 5009.137-2025".
Step 3: Click "Add to Cart". If multiple PDFs are required, repeat steps 2 and 3 to add up to 12 PDFs to cart.
Step 4: Select payment option (Via payment agents Stripe or PayPal).
Step 5: Customize Tax Invoice -- Fill up your email etc.
Step 6: Click "Checkout".
Step 7: Make payment by credit card, PayPal, Google Pay etc. After the payment is completed and in 9 seconds, you will receive 2 emails attached with the purchased PDFs and PDF-invoice, respectively.
Step 8: Optional -- Go to download PDF.
Step 9: Optional -- Click Open/Download PDF to download PDFs and invoice.
See screenshots for above steps: Steps 1~3 Steps 4~6 Step 7 Step 8 Step 9
|