GB 5009.128-2016 PDF English
US$90.00 · In stock · Download in 9 secondsGB 5009.128-2016: National food safety standard - Determination of cholesterol in foods Delivery: 9 seconds. True-PDF full-copy in English & invoice will be downloaded + auto-delivered via email. See step-by-step procedureStatus: Valid GB 5009.128: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivery | Name of Chinese Standard | Status |
| GB 5009.128-2016 | English | 90 |
Add to Cart
|
0-9 seconds. Auto-delivery
|
National food safety standard - Determination of cholesterol in foods
| Valid |
| GB/T 5009.128-2003 | English | 199 |
Add to Cart
|
2 days
|
Determination of cholesterin in foods
| Obsolete |
Excerpted PDFs (Download full copy in 9 seconds upon purchase)PDF Preview: GB 5009.128-2016
GB 5009.128-2016: National food safety standard - Determination of cholesterol in foods---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB5009.128-2016
GB
NATIONAL STANDARD OF
THE PEOPLE’S REPUBLIC OF CHINA
National food safety standard -
Determination of cholesterol in foods
Issued on. DECEMBER 23, 2016
Implemented on. JUNE 23, 2017
Issued by. National Health and Family Planning Commission of the
People's Republic of China;
China Food and Drug Administration.
Table of Contents
Foreword... 3
1 Scope... 4
2 Principle... 4
3 Reagents and materials... 4
4 Instruments and equipment... 5
5 Analysis steps... 5
6 Expression of analysis result... 7
7 Precision... 8
8 Other... 8
9 Principle... 8
10 Reagents and materials... 8
11 Instruments and equipment... 9
12 Analysis steps... 9
13 Expression of analysis result... 11
14 Precision... 12
15 Other... 12
16 Principle... 12
17 Reagents and materials... 12
18 Instruments and equipment... 14
19 Analysis steps... 14
20 Expression of analysis result... 15
21 Precision... 15
22 Other... 15
Annex A Cholesterol standard chromatogram... 16
Foreword
This Standard replaces GB/T 5009.128-2003 Determination of cholesterol in
foods, GB/T 22220-2008 Determination of cholesterol in foods - High-
performance liquid chromatography and GB/T 9695.24-2008 Meat and meat
products - Determination of cholesterol.
Compared with GB/T 5009.128-2003, the main changes in this Standard are as
follows.
- modified the standard’s name to “National food safety standard -
Determination of cholesterol in foods”;
- added gas chromatography as method one and high-performance liquid
chromatography as method two; modified colorimetric method as method
three;
- modified extraction solvent, the amount of ethanol and volume constant
volume in pre-treatment method of gas chromatography in GB/T 9695.24-
2008.
National food safety standard -
Determination of cholesterol in foods
1 Scope
This Standard specifies the determination methods of cholesterol in foods.
This Standard applies to the determination of cholesterol in foods. Method One
Gas chromatography is applicable to the determination of cholesterol in meat
and meat products, eggs and egg products, milk and dairy products and other
animal foods and vegetable oils and fats. Method Two High performance liquid
chromatography is applicable to the determination of cholesterol in meat and
meat products, eggs and egg products, milk and dairy products and other
animal foods. Method Three Colorimetric method is applicable to the
determination of cholesterol in meat and meat products, eggs and egg products
and other animal foods.
Method One -- Gas chromatography
2 Principle
Saponify the sample by absolute ethanol - potassium hydroxide solution.
Extract by petroleum ether and anhydrous ether mixture. Concentrate the
extract to dry. After dissolving and setting volume of absolute ethanol, use gas
chromatography to detect, external standard method to quantify.
3 Reagents and materials
Unless otherwise indicated, the reagents used in this method are of analytical
grade, water is grade one water specified in GB/T 6682.
3.1 Reagents
3.1.1 methanol (CH3OH). chromatographic pure
3.2 Regent preparation
3.2.1 60% potassium hydroxide solution. weigh 60 g of potassium hydroxide;
slowly add water to dissolve and set volume to 100 mL.
3.2.2 Petroleum ether - anhydrous ether mixture (1+1, volume ratio).
uniformly mix petroleum ether and anhydrous ether of equal volume.
3.3 Standard product
Cholesterol standard (C27H46O, CAS number. 57-88-5). purity ≥ 99%.
3.4 Preparation of standard solution
4 Instruments and equipment
4.1 Gas chromatograph. equipped with flame ionization detector (FID)
4.4 Saponification device
5 Analysis steps
5.1 Sample preparation
5.1.1 Meat and meat products and other types of solid samples
Take 200 g of edible part of sample to homogenize
5.1.2 Vegetable oil, dairy products and other liquid samples
Take the uniform liquid sample after mixing into a sealed container to be tested.
5.2 Sample processing
5.2.1 Saponification
Weigh 0.25g ~ 10g of prepared sample (nearest to 0.001 g, cholesterol content
of about 0.5mg ~ 5mg) in a 250-mL round-bottomed flask. Add 30 mL of
absolute ethanol, 10 mL of 60% potassium hydroxide solution.
5.2.2 Extraction
Quantitatively transfer of all saponified liquid in a 250-mL separatory funnel.
Use 30 mL of water to rinse the round-bottomed flask in 2~3 times. Merge the
washing liquid into the separatory funnel.
5.2.3 Concentration
Evaporate the extract in the flat-bottomed flask to near dryness under vacuum
condition. Use absolute ethanol to dissolve and set volume to 5 mL. Determine
with gas chromatograph.
The pretreatment of different sample requires blank test at the same time.
5.3 Determination
5.3.1 Instrument reference conditions
5.3.2 Production of standard curve
Inject the cholesterol standard series of working solution into the gas
chromatograph. Under the above chromatographic conditions, measure the
response value of the standard solution (peak area). Using concentration as
abscissa, the peak area as ordinate, make the standard curve.
6 Expression of analysis result
The content of cholesterol in the sample is calculated according to equation (1).
7 Precision
The absolute difference between two independent determinations obtained
under repeatability conditions shall not exceed 10% of the arithmetic mean.
8 Other
When the sample volume is 0.5 g, the set volume is 5.0 mL, the detection limit
shall be 0.3 mg/100g, the limit of quantification shall be 1.0 mg/100g.
9 Principle
Saponify the sample by absolute ethanol - potassium hydroxide solution.
Extract by petroleum ether and anhydrous ether mixture. Concentrate the
extract to dry.
10 Reagents and materials
Unless otherwise indicated, the reagents used in this method are of analytical
grade, water is grade one water specified in GB/T 6682.
10.1 Reagents
10.2 Regent preparation
10.2.1 60% potassium hydroxide solution. weigh 60 g of potassium hydroxide;
slowly add water to dissolve and set volume to 100 mL.
10.2.2 Petroleum ether - anhydrous ether mixture (1+1, volume ratio).
uniformly mix petroleum ether and anhydrous ether of equal volume.
10.3 Standard product
Cholesterol standard (C27H46O, CAS number. 57-88-5). purity ≥ 99%.
10.4 Preparation of standard solution
11 Instruments and equipment
11.1 Homogenizer
11.2 High performance liquid chromatography. equipped with UV detector
or equivalent detector
......
Source: Above contents are excerpted from the full-copy PDF -- translated/reviewed by: www.ChineseStandard.net / Wayne Zheng et al.
Tips & Frequently Asked QuestionsQuestion 1: How long will the true-PDF of English version of GB 5009.128-2016 be delivered?Answer: The full copy PDF of English version of GB 5009.128-2016 can be downloaded in 9 seconds, and it will also be emailed to you in 9 seconds (double mechanisms to ensure the delivery reliably), with PDF-invoice. Question 2: Can I share the purchased PDF of GB 5009.128-2016_English with my colleagues?Answer: Yes. The purchased PDF of GB 5009.128-2016_English will be deemed to be sold to your employer/organization who actually paid for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. www.ChineseStandard.us -- GB 5009.128-2016 -- Click this link and select your country/currency to pay, the exact amount in your currency will be printed on the invoice. Full PDF will also be downloaded/emailed in 9 seconds. Question 5: Should I purchase the latest version GB 5009.128-2016?Answer: Yes. Unless special scenarios such as technical constraints or academic study, you should always prioritize to purchase the latest version GB 5009.128-2016 even if the enforcement date is in future. Complying with the latest version means that, by default, it also complies with all the earlier versions, technically. How to buy and download a true PDF of English version of GB 5009.128-2016?A step-by-step guide to download PDF of GB 5009.128-2016_EnglishStep 1: Visit website https://www.ChineseStandard.net (Pay in USD), or https://www.ChineseStandard.us (Pay in any currencies such as Euro, KRW, JPY, AUD).
Step 2: Search keyword "GB 5009.128-2016".
Step 3: Click "Add to Cart". If multiple PDFs are required, repeat steps 2 and 3 to add up to 12 PDFs to cart.
Step 4: Select payment option (Via payment agents Stripe or PayPal).
Step 5: Customize Tax Invoice -- Fill up your email etc.
Step 6: Click "Checkout".
Step 7: Make payment by credit card, PayPal, Google Pay etc. After the payment is completed and in 9 seconds, you will receive 2 emails attached with the purchased PDFs and PDF-invoice, respectively.
Step 8: Optional -- Go to download PDF.
Step 9: Optional -- Click Open/Download PDF to download PDFs and invoice.
See screenshots for above steps: Steps 1~3 Steps 4~6 Step 7 Step 8 Step 9
|