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GB 5009.124-2016

Chinese Standard: 'GB 5009.124-2016'
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GB 5009.124-2016English90 Add to Cart 0--10 minutes. Auto-delivered. National Food Safety Standard -- Determination of Amino Acid in Foods GB 5009.124-2016 Valid GB 5009.124-2016
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Standard ID GB 5009.124-2016 (GB5009.124-2016)
Description (Translated English) Determination of amino acids in foods
Sector / Industry National Standard
Classification of Chinese Standard X09
Date of Issue 2016-12-23
Date of Implementation 2017-06-23
Older Standard (superseded by this standard) GB/T 5009.124-2003
Regulation (derived from) National Health and Family Planning Commission Notice No.17 of 2016

GB 5009.124-2016
National Food Safety Standard
– Determination of Amino Acid in Foods
食品安全国家标准 食品中氨基酸的测定
Issued by. National Health and Family Planning Commission of PRC;
China Food and Drug Administration.
Table of Contents
Foreword ... 3
1 Scope ... 4
2 Principle ... 4
3 Reagents and Materials ... 4
4 Instrument and Equipment ... 5
5 Analytical Procedures ... 6
6 Expression of Analytical Results ... 8
7 Precision ... 10
8 Others ... 10
Appendix A Chromatogram ... 12
This Standard replaces GB/T 5009.124-2003 Determination of Amino Acid in Foods.
Compared with GB/T 5009.124-2003, this Standard has the major changes as follows.
--- Modify the standard name into “National Food Safety Standard – Determination
of Amino Acid in Foods”;
--- Expand the applicable scope;
--- Add the limit of detection and quantitative limit of the method;
--- Modify the result calculation formula.
National Food Safety Standard
– Determination of Amino Acid in Foods
1 Scope
This Standard specifies using the amino acid analyzer (ninhydrin post-column
derivatization ion exchange chromatography) to determine the amino acid in foods.
This Standard is applicable to determine the amino acid hydrolyzed by acid in foods,
there are totally 16 kinds of amino acids such as aspartic acid, threonine, serine,
glutamic acid, proline, glycine, alanine, valine, methionine, isoleucine, leucine, tyrosine,
phenylalanine, histidine, lysine, and arginine.
2 Principle
The protein in food is hydrolyzed by hydrochloric acid into free amino acid; after being
separated by ion exchange column, it occurs color reaction with ninhydrin solution;
then determine the amino acid content through the visible light spectrophotometer.
3 Reagents and Materials
Unless otherwise specified, the reagents used in this method are analytically pure;
while water is Class-I water stipulated in GB/T 6682.
3.1 Reagents
3.1.1 Hydrochloric acid (HCl). concentration≥36%, guarantee reagent.
3.1.2 Phenol (C6H5OH).
3.1.3 Nitrogen. with purity of 99.9%.
3.1.4 Sodium citrate (Na3C6H5O7•2H2O). guarantee reagent.
3.1.5 Sodium hydroxide (NaOH). guarantee reagent.
3.2 Reagents preparation
3.2.1 Hydrochloric acid solution (6mol/L). take 500mL of hydrochloric acid, dilute with
water to 1000mL, mix evenly.
3.2.2 Refrigerant. mix the commercial salt and ice by the mass ratio of 1.3.
Place the hydrolysis tube into the refrigerant, frozen for 3min~5min; connect to the
suction tube of vacuum pump; pump vacuum (close to 0Pa); then inject nitrogen;
repeatedly pump vacuum, inject nitrogen, after 3 times, seal the cap or tighten the
screw cap under nitrogen injecting state.
Place the sealed hydrolysis tube into 110°C±1°C electric blower thermostat or
hydrolysis furnace; hydrolyze for 22h, then take out; cooling off to the room
Open the hydrolysis tube, filter the hydrolysate into 50mL volumetric flask; use small
amount of water to wash the hydrolysis tube for several times; transfer the washing
liquid into the same 50mL volumetric flask; finally use water to make constant volume
to the scale; shake and mix evenly.
Accurately pipette 1.0mL of filtrate into 15mL or 25mL test tube; use the test tube
concentrator or parallel evaporator to reduce pressure to dry at the 40°C~50°C heating
temperature environment; after drying, the residuals shall use 1mL~2mL of water to
dissolve; then reduce pressure to dry; finally evaporate to dryness.
Add 1.0mL~2.0mL of sodium citrate buffer solution with pH 2.2 into the test tube after
drying to dissolve; after shaking and mixing evenly; absorb the solution to get through
the 0.22µm filter film; transfer into the instrument sample-injecting bottle; it is the
sample assay solution for the measurement of the instrument.
5.4 Determination
5.4.1 Instrument conditions
Inject the mixed amino acid standard working solution into the automatic amino acid
analyzer; refer to the test protocol and instrument manual of the amino acid analyzer
stipulated in JJG1064-2011; appropriately adjust the instrument operation procedures,
parameters, and reagent ratio of buffer so......
Related standard: GB 5009.118-2016    GB 5009.138-2017
Related PDF sample: GB/T 5009.124-2003    GB 5009.121-2016