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GB 4789.1-2016 English PDF (GB 4789.1-2010, GB/T 4789.1-2008)

GB 4789.1-2016_English: PDF (GB4789.1-2016)
Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
GB 4789.1-2016English70 Add to Cart 0--9 seconds. Auto-delivery National Food Safety Standard -- Food Microbiological Examination -- General Valid GB 4789.1-2016
GB 4789.1-2010English70 Add to Cart 0--9 seconds. Auto-delivery National food safety standard -- Food microbiological examination: General guidelines Obsolete GB 4789.1-2010
GB/T 4789.1-2008English279 Add to Cart 3 days [Need to translate] Microbiological examination of food hygiene -- General guidelines Obsolete GB/T 4789.1-2008
GB/T 4789.1-2003English199 Add to Cart 2 days [Need to translate] Microbiological examination of food hygiene -- General principles Obsolete GB/T 4789.1-2003
GB 4789.1-1994EnglishRFQ ASK 3 days [Need to translate] Microbiological examination of food hygiene. General principles Obsolete GB 4789.1-1994
GB 4789.1-1984EnglishRFQ ASK 3 days [Need to translate] Microbiological examination of food hygiene--General principles Obsolete GB 4789.1-1984


BASIC DATA
Standard ID GB 4789.1-2016 (GB4789.1-2016)
Description (Translated English) National Food Safety Standard -- Food Microbiological Examination -- General
Sector / Industry National Standard
Classification of Chinese Standard C53
Classification of International Standard 07.100.30
Word Count Estimation 7,782
Date of Issue 2016-12-23
Date of Implementation 2017-06-23
Older Standard (superseded by this standard) GB 4789.1-2010
Regulation (derived from) National Health and Family Planning Commission Notice No.17 of 2016
Issuing agency(ies) National Health and Family Planning Commission of the People Republic of China, State Administration of Food and Drug Administration
Summary This standard specifies the basic principles and requirements for microbiological testing of food. This standard applies to food microbiological examination.

BASIC DATA
Standard ID GB 4789.1-2010 (GB4789.1-2010)
Description (Translated English) National food safety standard - Food microbiological examination: General guidelines
Sector / Industry National Standard
Classification of Chinese Standard C53
Classification of International Standard 07.100.30
Word Count Estimation 6,648
Date of Issue 2010-03-26
Date of Implementation 2010-06-01
Older Standard (superseded by this standard) GB/T 4789.1-2008
Quoted Standard GB/T 4789.28; GB 19489
Regulation (derived from) Circular of the Ministry of Health (2010)7
Issuing agency(ies) Ministry of Health of People's Republic of China
Summary This Chinese standard specifies the basic principles of food microbiology tests and requirements. This standard applies to food microbiology.

BASIC DATA
Standard ID GB/T 4789.1-2008 (GB/T4789.1-2008)
Description (Translated English) Microbiological examination of food hygiene. General guidelines
Sector / Industry National Standard (Recommended)
Classification of Chinese Standard C53
Classification of International Standard 07.100.30
Word Count Estimation 7,740
Date of Issue 2008-11-21
Date of Implementation 2009-03-01
Older Standard (superseded by this standard) GB/T 4789.1-2003
Quoted Standard GB/T 4789; GB/T 4789.28; GB 14938; GB 19489
Drafting Organization Chinese Center for Disease Control Top Anti Nutrition and Food Safety
Administrative Organization Ministry of Health
Regulation (derived from) National Standard Approval Announcement 2008 No.22 (Total No.135); Health-Communication (2010) 7; National Standard Approval Announcement 2010 No.5 (Total No.160)
Proposing organization People's Republic of China Ministry of Health
Issuing agency(ies) Ministry of Health of People's Republic of China; Standardization Administration of China
Summary This standard specifies the basic Microbiological examination of food hygiene principles and requirements. This standard applies to Microbiological examination of food hygiene.


GB 4789.1-2016 GB NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA National Food Safety Standard – Food Microbiological Examination. General Guideline ISSUED ON. DECEMBER 23, 2016 IMPLEMENTED ON. JUNE 23, 2017 Issued by. National Health and Family Planning Commission of the PRC; China Food and Drug Administration. 3. No action is required - Full-copy of this standard will be automatically & immediately delivered to your EMAIL address in 0~60 minutes. Table of Contents Foreword ... 3 1 Scope ... 4 2 Laboratory Basic Requirements ... 4 3 Sample Collection ... 6 4 Examination ... 8 5 Biosafety and Quality Control ... 8 6 Record and Report ... 9 7 Sample Treatment after Examination ... 9 Appendix A Microbiology Laboratory Routine Examination Supplies and Equipment ... 10 Foreword This Standard replaced GB 4789.1-2010 National Food Safety Standard – Food Microbiological Examination. General Guideline. Compared with GB 4789.1-2010, this Standard has the major changes as follows. --- Add the Appendix A, Microbiological Laboratory Routine Examination Supplies and Equipment; --- Modify the laboratory basic requirements; --- Modify the sample collection; --- Modify the inspection; --- Modify the sample treatment after inspection; --- Delete the normative references. National Food Safety Standard – Food Microbiological Examination. General Guideline 1 Scope This Standard specifies the basic principles and requirements for food microbiological examination. This Standard is applicable to the food microbiological examination. 2 Laboratory Basic Requirements 2.1 Inspectors 2.1.1 He/she shall Have the corresponding microbiological professional education or training experience; have the corresponding qualification, and can understand and correctly perform the examination. 2.1.2 He/she shall master the laboratory biosafety operation and disinfection knowledge. 2.1.3 He/she shall, during the examination process, keep the personal clean and tidy; prevent the artificial pollution of the sample. 2.1.4 He/she shall, during the examination process, abide by the relevant safety measure provisions; and ensure his/her own safety. 2.1.5 The personnel who has color deficiencies shall not engage in the experiments for color discrimination. 2.2 Environment and facilities 2.2.1 The laboratory environment shall not affect the accuracy of the test results. 2.2.2 The experimental area shall be clearly separated from the office area. 2.2.3 The working area and overall layout of the laboratory shall meet the requirements of engaging in the examination; the laboratory layout shall adopt the one- way working flow; prevent the cross-pollution. 2.2.4 The temperature, humidity, cleanliness and illumination and noise, etc. of the ≤c pieces of samples are allowed to be between m value and M value; the corresponding microbial indicator examination value of no samples are allowed to exceed M value. For instance. n=5, c=2, m=100CFU/g, M=1000CFU/g. It means that collect 5 samples from 1 batch of products; if the examination results of the 5 samples are all less than or equal to m value (≤100CFU/g), such conditions are allowable; if the examination results of ≤ 2 samples (X) are between m value and M value (100CFU/g< X≤1000CFU/g), such conditions are also allowable; if the examination results of 3 and above samples are between m value and M value, such conditions are unallowable; if the examination results of any sample are greater than M value (>1000CFU/g), such conditions are unallowable. 3.2.3 The sampling solutions of various foods shall be carried out as per the provisions of relevant food standards. 3.2.4 Food sample collection in the food safety accident. a) If the food safety accident is caused by food pollution during the batch production and processing, the food sample shall be collected and judged as per the principles in 3.2.2 and 3.2.3. The same batch of food samples shall be focused on to collect. b) If the food safety accident is caused by restaurant or family cooking or processing, the remaining food samples shall be focused on to collect; so that meet the requirements of etiology and pathogen confirmation. 3.3 Sampling method of various foods 3.3.1 Pre-packaged food 3.3.1.1 The food samples with the same batch, independent package, and appropriate quantity shall be collected; the sampling amount of each sample shall meet the requirements of microbial indicator examination. 3.3.1.2 The solid food with independent package weight less than or equal to 1000g, or the liquid food with volume less than or equal to 1000mL shall take the sample from the same batch of package. 3.3.1.3 Shake or use sterile bar to mix the liquid food with independent package greater than 1000mL before sampling, after it becomes homogeneous, take appropriate amount of sample; put it into the sterile sampling container as a food sample. For the solid food greater than 1000g, use the sterile collector to take an appropriate amount of sample from different parts of the same package; then put into the same sterile sampling container as a food sample. 3.3.2 Bulk food or one-site produced food Use the sterile sampling tool to take sample on-site from n different parts; put then into Appendix A Microbiology Laboratory Routine Examination Supplies and Equipment A.1 Equipment A.1.1 Weighing device. balance, etc. A.1.2 Disinfection and sterilization device. dry baking/drying device, autoclaving device, filtration and sterilization device, ultraviolet and other devices. A.1.3 Culture medium preparation device. pH meter, etc. A.1.4 Sample treating device. homogenizer (shear or beat homogenizer), centrifuge, etc. A.1.5 Diluting device. pipettes, etc. A.1.6 Cultivating device. constant temperature incubator, constant temperature water bath, etc. A.1.7 Microscopic counting device. microscope, magnifier, Vernier caliper, etc. A.1.8 Refrigerating device. refrigerator, freezer, etc. A.1.9 Biosafety device. biosafety cabinet. A.1.10 Other devices. A.2 Examination supplies A.2.1 Routine examination supplies. inoculation ring (needle), alcohol lamp, tweezers, scissors, spoon, sterile cotton ball, silica-gel (cotton) plug, suction tube, suction ball, test tube, flat plate, conical flask, wide jar, micro-hole plate, measuring cylinder, glass rod, L-shaped glass rod, pH test paper, marker, homogeneous bag, etc. A.2.2 Examination supplies for on-site sampling. sterile sampling container, cotton swabs, smudge stick, sampling specification board, transit tube, etc. ......


GB 4789.1-2010 NATIONAL STANDARD OF THE PEOPLE’S REPUBLIC OF CHINA National Food Safety Standard Food Microbiological Examination. General Guidelines ISSUED ON. MARCH 26, 2010 IMPLEMENTED ON. JUNE, 1, 2010 Issued by. Ministry of Health of the People’s Republic of China GB Tips - GB 4789 Series (Not part of this Standard) Standard ID Standard Name Issued Date Enforced Date New Version (Click to check)? GB 4789.1-2010 National food safety standard Food microbiological examination. General guidelines 2010-03-26 2010-06-01 GB 4789.2-2010 National food safety standard Food microbiological examination. Aerobic plate count 2010-03-26 2010-06-01 GB 4789.3-2010 National food safety standard Food microbiological examination. Enumeration of coliforms 2010-03-26 2010-06-01 GB 4789.4-2010 National food safety standard Food microbiological examination. Salmonella 2010-03-26 2010-06-01 GB 4789.5-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Shigella 2012-05-17 2012-07-17 GB/T 4789.6- Microbiological examination of food hygiene Examination of diarrheogenic Escherichia coli 2003-08-11 2004-01-01 GB/T 4789.7- Microbiological examination of food hygiene. Examination of Vibrio parahaemolyticus 2008-05-16 2008-11-01 GB/T 4789.8- Microbiological examination of food hygiene. Examination of Yersinia enterocolitica 2008-11-21 2009-03-01 GB/T 4789.9- Microbiological examination of food hygiene. Examination of Campylobacter jejuni 2008-11-21 2009-03-01 GB 4789.10-2010 National food safety standard Food microbiological examination. Staphylococcus aureus 2008-11-21 2009-03-01 GB/T 4789.11- Microbiological examination of food hygiene Examination of streptococcus hemolyticus 2003-08-11 2004-01-01 GB/T 4789.12- Microbiological examination of food hygiene Examination of Clostridium botulinum and botulinus toxin 2003-08-11 2004-01-01 GB 4789.13-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Clostridium Perfringens 2012-05-17 2012-07-17 GB/T 4789.14- Microbiological examination of food hygiene Examination of Bacillus cereus 2003-08-11 2004-01-01 GB 4789.15-2010 National food safety standard Food microbiological examination. Enumeration of moulds and yeasts 2010-03-26 2010-06-01 GB/T 4789.16- Microbiological examination of food hygiene Identification of common mycotoxin producing fungi 2003-08-11 2004-01-01 GB/T 4789.17- Microbiological examination of food hygiene Examination of meat and meat products 2003-08-11 2004-01-01 GB 4789.18-2010 National food safety standard Food microbiological examination. Milk and milk products 2010-03-26 2010-06-01 GB/T 4789.19- Microbiological examination of food hygiene Examination of egg and egg products 2003-08-11 2004-01-01 GB/T 4789.20- Microbiological examination of food hygiene Examination of aquatic product foods 2003-08-11 2004-01-01 GB/T 4789.21- Microbiological examination of food hygiene Examination of frozen drinks and cold drinks 2003-08-11 2004-01-01 GB/T 4789.22- Microbiological examination of food hygiene Examination of flavourings 2003-08-11 2004-01-01 GB/T 4789.23- Microbiological examination of food hygiene Examination of cold dish and bean products 2003-08-11 2004-01-01 GB/T 4789.24- Microbiological examination of food hygiene Examination of candy, cake and preserved fruits 2003-08-11 2004-01-01 GB/T 4789.25- Microbiological examination of food hygiene Examination of wines 2003-08-11 2004-01-01 GB/T 4789.26- Microbiological examination of food hygiene Examination of commercial sterilization of canned food 2003-08-11 2004-01-01 GB/T 4789.27- Microbiological examination of food hygiene. Examination of residue of antibiotics in fresh milk 2008-11-21 2009-03-01 GB/T 4789.28- Microbiological examination of food hygiene Staining methods, culture mediums and reagents 2003-08-11 2004-01-01 GB/T 4789.29- Microbiological examination of food hygiene Examination of pseudomonas cocovenenans subsp. farinofermentans 2003-08-11 2004-01-01 GB 4789.30-2010 National food safety standard Food microbiological examination. Listeria monocytogenes 2010-03-26 2010-06-01 GB/T 4789.31- Microbiological examination of food hygiene Examination of salmonellae, shigellae, and diarrhoea causative Escherichia coli by means of the diagnostic typing phage set for enterobacteriaceae GB/T 4789.32- Microbiological examination for food hygiene rapid detection of coliform bacteria 2002-06-13 2002-10-01 GB/T 4789.33- Microbiological examination of food hygiene Examination of cereal, fruit and vegetable 2003-08-11 2004-01-01 GB 4789.34-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Examination of Bifidobacterium 2012-05-17 2012-07-17 GB 4789.35-2010 National food safety standard Food microbiological examination. Lactic acid bacteria 2010-03-26 2010-06-01 GB/T 4789.36- Microbiological examination of food hygiene. Examination of Escherichia coli O157. H7/NM 2008-05-16 2008-11-01 GB/T 4789.37- Microbiological examination of food hygiene. Examination of Staphylococcus aureus 2008-11-21 2009-03-01 GB 4789.38-2012 National Food Safety Standard Microbiological Examination of Food Hygiene - Enumeration of Escherichia Coli 2012-05-17 2012-07-17 GB/T 4789.39- Microbiological examination of food hygiene. Enumeration of faecal coliforms 2008-11-21 2009-03-01 GB 4789.40-2010 National food safety standard Food microbiological examination. Enterobacter sakazakii 2010-03-26 2010-06-01 Table of Contents Foreword ...7 1 Scope ...8 2 Normative references ...8 3 General laboratory requirements ...8 4 Sample collection ...10 5 Sample examination ...13 6 Biosafety and quality control...14 7 Recording and reporting ...14 8 Disposal of samples after examination ...14 Foreword This Standard replaces GB/T 4789.1-2008 Microbiological Examination of Food Hygiene – General Guidelines. The main changes in this Standard over GB/T 4789.1-2008 are as follows. - Modify the standard name of both English and Chinese; - Modify the selection of examination methods. The historical editions replaced by this Standard are as follows. - GB 4789.1-1984, GB 4789.1-1994, GB 4789.1-2003, and GB 4789.1-2008. National food safety standard Food microbiological examination. General guidelines 1 Scope This Standard specifies the general principles and requirements for food microbiological examination. This Standard is applicable to the food microbiological examination. 2 Normative references Documents referenced in this Standard are indispensable for application of this Standard. For dated references, only the dated edition is applicable to this Standard. For undated references, the latest edition (including all amendments) is applicable to this Standard. 3 General laboratory requirements 3.1 Environment 3.1.1 The laboratory environment shall not affect the accuracy of examination results. 3.1.2 The operation zone and the office zone of the laboratory shall be obviously separated from each other. 3.1.3 The operation zone and general layout of the laboratory shall meet the requirements for examination, and the laboratory layout shall adopt one-way work process to avoid cross contamination. 3.1.4 Ambient temperature, humidity, luminance, noise, and cleanliness etc. of the laboratory shall meet working requirements. 3.1.5 General sample examination shall be performed in clean zone (including super-clean bench or clean laboratory), clean zone shall have visible markings. 3.1.6 Separation and identification of pathogenic microbes shall be performed in laboratories of Biosafety level 2 (BSL-2). 3.2 Personnel 3.2.1 Examination personnel shall have related education or professional microbial training experience, with appropriate qualifications, and can understand and correctly implement the examination. 3.4.4 The items and materials used repeatedly (e.g. culture dish, suction tube, sucker, test tube and inoculating loop, etc.) can be replaced with disposable items applicable to the microbiological examination. 3.4.5 The storage environment for the examination appliances shall be kept dry and clean. The sterilized and unsterilized appliances shall be stored separately and clearly labeled. 3.4.6 The sterilization/disinfection temperature and duration shall be recorded for sterilized examination appliances. 3.5 Culture medium and reagents 3.5.1 Culture medium The preparation and quality control of culture medium shall follow the provisions of GB/T 4789.28. 3.5.2 Reagents The quality and preparation of examination reagents shall be applicable to relevant examination... ......


GB/T 4789.1-2008 Microbiological examination of food hygiene. General guidelines ICS 07.100.30 C53 National Standards of People's Republic of China Replacing GB/T 4789.1-2003 Microbiological examination of food hygiene - General Posted 2008-11-21 2009-03-01 implementation People's Republic of China Ministry of Health Standardization Administration of China released Foreword This standard replaces GB/T 4789.1-2003 "General Microbiological examination of food hygiene." This standard compared with GB/T 4789.1-2003 The main changes are as follows. --- Increasing the microbiology laboratory of the basic requirements, including the environment, personnel, equipment; --- Modify the sample collection, increasing the sampling scheme and the type of determination; --- Modify the sample inspection, increased quality control; --- Increased processing test samples. This standard is proposed and administered by the People's Republic of China Ministry of Health. This standard by the People's Republic of China Ministry of Health is responsible for interpretation. This standard is drafted by. China Center for Disease Control Nutrition and Food Safety. Participated in the drafting of this standard. Inner Mongolia People's Republic of China Exit Inspection and Quarantine, the Shanghai Municipal Center for Disease Control and Prevention, China Jiangsu People's Republic of Exit Inspection and Quarantine, CDC, Zhejiang Province, People's Republic of China Liaoning Entry-Exit Inspection and Quarantine Bureau. The main drafters of this standard. Xiumei, Liu middle school, Liu Hong, Tian Jing, Guo Yunchang, Zhu Changqing, Chengsu Yun, Lu Hangan, Wang Jun. The standard version of the calendar year instead of the standard release of case. --- GB 4789.1-1984, GB/T 4789.1-1994, GB/T 4789.1-2003. Microbiological examination of food hygiene - General 1 Scope This standard specifies the Microbiological examination of food hygiene basic principles and requirements. This standard applies to Microbiological examination of food hygiene. 2 Normative references The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. GB/T 4789 (all parts) Microbiological examination of food hygiene GB/T 4789.28 Microbiological examination of food hygiene staining, media and reagents GB 14938 food poisoning diagnostic criteria and technical General GB 19489 General requirements for laboratory biosafety 3 laboratory basic requirements 3.1 Environment 3.1.1 The laboratory environment should not affect the accuracy of test results. 3.1.2 The laboratory work area and office area should be clearly separated. 3.1.3 The laboratory work area and overall layout should be able to meet the needs of engaged in inspection work, the laboratory should use the layout of one-way workflow Cheng, avoid cross-contamination. 3.1.4 laboratory temperature, humidity, illumination, noise and cleanliness, etc. should meet the job requirements. 3.1.5 General test sample should be in a clean area [including clean bench or clean laboratory (partial one hundred)] were, clean area should be clear Significant identity. 3.1.6 Isolation and identification of pathogenic micro-organisms should be carried out in two biosafety laboratory (biosafetylevel2, BSL-2) in. 3.2 staff 3.2.1 The inspectors should have the appropriate education, professional training experience microbes, have the appropriate qualifications, can be understood and properly implemented test. 3.2.2 The inspectors should have a working knowledge of laboratory bio-safety testing and disinfection knowledge. 3.2.3 Inspection personnel should maintain personal cleanliness and hygiene in the inspection process to prevent human contamination of the sample. 3.2.4 Inspection personnel shall comply with the relevant precautions in the inspection process, to ensure their own safety. 3.2.5 color visually impaired person can not perform experiments related to color vision. 3.3 Device 3.3.1 The test equipment should meet the needs of inspection work. 3.3.2 The laboratory equipment should be placed in appropriate environmental conditions, ease of maintenance, cleaning, disinfection and calibration, and kept clean and in good working status. 3.3.3 The laboratory equipment should be regularly checked, test (add logo), maintenance and repair, in order to ensure the performance and operational safety. 3.3.4 laboratory equipment should be routine monitoring records and use records. 3.4 Test Supplies 3.4.1 routine testing supplies mainly inoculation loop (needle), alcohol lamp, tweezers, scissors, spoon, sterile cotton balls, silica gel (cotton) plug micropipette ......

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