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GB 4789.1-2016

Chinese Standard: 'GB 4789.1-2016'
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BASIC DATA
Standard ID GB 4789.1-2016 (GB4789.1-2016)
Description (Translated English) National food safety standard Food microbiological examination: General guidelines
Sector / Industry National Standard
Classification of Chinese Standard C53
Classification of International Standard 07.100.30
Word Count Estimation 7,759
Date of Issue 2016-12-23
Date of Implementation 2017-06-23
Older Standard (superseded by this standard) GB 4789.1-2010
Regulation (derived from) National Health and Family Planning Commission Notice No.17 of 2016
Issuing agency(ies) National Health and Family Planning Commission of the People Republic of China, State Administration of Food and Drug Administration
Summary This standard specifies the basic principles and requirements for microbiological testing of food. This standard applies to food microbiological examination.

GB 4789.1-2016
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
National Food Safety Standard –
Food Microbiological Examination. General Guideline
食品安全国家标准 食品微生物学检验 总则
ISSUED ON. DECEMBER 23, 2016
IMPLEMENTED ON. JUNE 23, 2017
Issued by. National Health and Family Planning Commission of the PRC;
China Food and Drug Administration.
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Table of Contents
Foreword ... 3
1 Scope ... 4
2 Laboratory Basic Requirements ... 4
3 Sample Collection ... 6
4 Examination ... 8
5 Biosafety and Quality Control ... 8
6 Record and Report ... 9
7 Sample Treatment after Examination ... 9
Appendix A Microbiology Laboratory Routine Examination Supplies and
Equipment ... 10
Foreword
This Standard replaced GB 4789.1-2010 National Food Safety Standard – Food
Microbiological Examination. General Guideline.
Compared with GB 4789.1-2010, this Standard has the major changes as follows.
--- Add the Appendix A, Microbiological Laboratory Routine Examination Supplies
and Equipment;
--- Modify the laboratory basic requirements;
--- Modify the sample collection;
--- Modify the inspection;
--- Modify the sample treatment after inspection;
--- Delete the normative references.
National Food Safety Standard –
Food Microbiological Examination. General Guideline
1 Scope
This Standard specifies the basic principles and requirements for food microbiological
examination.
This Standard is applicable to the food microbiological examination.
2 Laboratory Basic Requirements
2.1 Inspectors
2.1.1 He/she shall Have the corresponding microbiological professional education
or training experience; have the corresponding qualification, and can understand and
correctly perform the examination.
2.1.2 He/she shall master the laboratory biosafety operation and disinfection
knowledge.
2.1.3 He/she shall, during the examination process, keep the personal clean and tidy;
prevent the artificial pollution of the sample.
2.1.4 He/she shall, during the examination process, abide by the relevant safety
measure provisions; and ensure his/her own safety.
2.1.5 The personnel who has color deficiencies shall not engage in the experiments
for color discrimination.
2.2 Environment and facilities
2.2.1 The laboratory environment shall not affect the accuracy of the test results.
2.2.2 The experimental area shall be clearly separated from the office area.
2.2.3 The working area and overall layout of the laboratory shall meet the
requirements of engaging in the examination; the laboratory layout shall adopt the one-
way working flow; prevent the cross-pollution.
2.2.4 The temperature, humidity, cleanliness and illumination and noise, etc. of the
≤c pieces of samples are allowed to be between m value and M value; the corresponding
microbial indicator examination value of no samples are allowed to exceed M value.
For instance. n=5, c=2, m=100CFU/g, M=1000CFU/g. It means that collect 5 samples
from 1 batch of products; if the examination results of the 5 samples are all less than
or equal to m value (≤100CFU/g), such conditions are allowable; if the examination
results of ≤ 2 samples (X) are between m value and M value
(100CFU/gresults of 3 and above samples are between m value and M value, such conditions are
unallowable; if the examination results of any sample are greater than M value
(>1000CFU/g), such conditions are unallowable.
3.2.3 The sampling solutions of various foods shall be carried out as per the
provisions of relevant food standards.
3.2.4 Food sample collection in the food safety accident.
a) If the food safety accident is caused by food pollution during the batch production
and processing, the food sample shall be collected and judged as per the principles
in 3.2.2 and 3.2.3. The same batch of food samples shall be focused on to collect.
b) If the food safety accident is caused by restaurant or family cooking or processing,
the remaining food samples shall be focused on to collect; so that meet the
requirements of etiology and pathogen confirmation.
3.3 Sampling method of various foods
3.3.1 Pre-packaged food
3.3.1.1 The food samples with the same batch, independent package, and
appropriate quantity shall be collected; the sampling amount of each sample shall meet
the requirements of microbial indicator examination.
3.3.1.2 The solid food with independent package weight less than or equal to 1000g,
or the liquid food with volume less than or equal to 1000mL shall take the sample from
the same batch of package.
3.3.1.3 Shake or use sterile bar to mix the liquid food with independent package
greater than 1000mL before sampling, after it becomes homogeneous, take
appropriate amount of sample; put it into the sterile sampling container as a food
sample. For the solid food greater than 1000g, use the sterile collector to take an
appropriate amount of sample from different parts of the same package; then put into
the same sterile sampling container as a food sample.
3.3.2 Bulk food or one-site produced food
Use the sterile sampling tool to take sample on-site from n different parts; put then into
Appendix A
Microbiology Laboratory Routine
Examination Supplies and Equipment
A.1 Equipment
A.1.1 Weighing device. balance, etc.
A.1.2 Disinfection and sterilization device. dry baking/drying device, autoclaving
device, filtration and sterilization device, ultraviolet and other devices.
A.1.3 Culture medium preparation device. pH meter, etc.
A.1.4 Sample treating device. homogenizer (shear or beat homogenizer), centrifuge,
etc.
A.1.5 Diluting device. pipettes, etc.
A.1.6 Cultivating device. constant temperature incubator, constant temperature
water bath, etc.
A.1.7 Microscopic counting device. microscope, m......
Related standard:   GB 4789.10-2016  GB 4789.12-2016
Related PDF sample:   GB 4789.2-2016  GB 4789.3-2016
   
 
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