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GB 19641-2015

Chinese Standard: 'GB 19641-2015'
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GB 19641-2015English179 Add to Cart Days<=3 National food safety standard -- Oil seeds Valid GB 19641-2015
GB 19641-2015Chinese17 Add to Cart <=1-day [PDF from Chinese Authority, or Standard Committee, or Publishing House]


Standard ID GB 19641-2015 (GB19641-2015)
Description (Translated English) (National Food Safety Standard edible oil)
Sector / Industry National Standard
Classification of Chinese Standard X09
Classification of International Standard 67.040
Word Count Estimation 9,924
Date of Issue 2015-11-13
Date of Implementation 2016-11-13
Older Standard (superseded by this standard) GB 19641-2005
Regulation (derived from) National Health and Family Planning Commission Announcement No
Issuing agency(ies) National Health and Family Planning Commission of the People's Republic of China

GB 19641-2015
(National Food Safety Standard edible oil)
National Standards of People's Republic of China
National Food Safety Standard
Edible oil
Issued on. 2015-11-13
2016-11-13 implementation
People's Republic of China
National Health and Family Planning Commission released
This standard replaces GB 19641-2005 "oil plant health standards."
This standard compared with GB 19641-2005, the main changes are as follows.
--- Standard name was changed to "national food safety standards edible oil";
--- Modify the sensory requirements;
--- Modify the physical and chemical properties;
--- Added Appendix.
National Food Safety Standard
Edible oil
1 Scope
This standard applies to the preparation of edible vegetable oil.
2 Terms and definitions
2.1 moldy grain
Grain mildew and obviously hurt the embryo or endosperm or cotyledons, no edible particles value.
3 Technical requirements
3.1 Sensory requirements
Sensory requirements shall comply with the requirements of Table 1.
Table 1 Sensory requirements
Item Index Test Method
Color, color, odor of oil with normal odor GB/T 5492
Moldy grain /%
Soybean ≤
Other ≤
According to GB/T 5494 in imperfect grain inspection regulations
Set, and sort out the moldy grain, is weighed, calculated
3.2 poisonous and harmful fungi and plant seeds Limited
Toxic and harmful fungi and seeds should be limited in accordance with Table 2.
Table 2 toxic and harmful fungi and plant seeds Limited
Item Index Test Method
Datura seed and other poisonous plants a/(tablets/kg)
Soybean, rapeseed ≤ 1
Appendix A
Ergot /%
Rapeseed ≤
Not Detected
Appendix B
a Crotalaria (Crotalariaspp.), wheat Seno (AgrostemmagithagoL.), castor bean (RicinuscommunisL.) and other recognized for
Harmful to the health of the seeds.
3.3 Limits of contaminants and mycotoxins Limited
3.3.1 Limits of contaminants shall comply with the provisions of GB 2762.
3.3.2 Limits of Mycotoxins shall comply with the provisions of GB 2761.
3.4 MRL
MRL shall comply with the provisions of GB 2763.
4 Other
Transgenic edible oil identification should comply with the relevant provisions of the State.
Appendix A
Datura seed testing methods
A.1 Identification
A.1.1 morphology
Datura seed round, rectangular, kidney-shaped, triangular kidney-shaped, oval-shaped broadly ovate, seed length 3mm ~ 5mm, width 2.5mm ~
4.0mm, both sides of the flat, the back thicker or thick, smooth or wavy edge ridges. Species leathery, pale yellow, brown, tan to dark brown,
The surface is slightly wrinkled, or slightly (obviously) concave, with (or without) and coarse textured pocket. Hilum, long triangle, equilateral triangle or T-shaped, sometimes its surface often
Covered with remnants of white suspensor. Seed contains a wealth of white endosperm, germ or polycyclic raw campylotropous, few straight students. Figure A.1 for all types of Datura
Seed photo.
Figure A.1 Datura seed photo [1]
A.1.2 determination
A.1.1 can be identified in line with the above described morphological characteristics of Datura.
A.2 alkaloids colorimetric characterization
A.2.1 Principle
Samples have color reaction with fuming nitric acid and potassium hydroxide solution was contained atropine and other alkaloids were extracted.
A.2.2 Reagents
A.2.2.1 ammonia (11).
A.2.2.2 ether.
A.2.2.3 hydrochloric acid (15).
A.2.2.4 chloroform.
A.2.2.5 anhydrous sodium sulfate.
A.2.2.6 fuming nitric acid.
A.2.2.7 KOH - ethanol solution (100g/L).
A.2.3 Analysis step
About 30 datura seeds into a mortar, add ammonia (11) soaked, soaking moment, ground into viscous, abrasive with ether three times, each
Times 10mL, ether combined in a separating funnel, add 10mL hydrochloric acid (15), shaking extraction 1min, hydrochloric acid layer was separated by liquid separation to another leak
Bucket, add ammonia (11) made basic with 10mL chloroform shaking extraction 1min, repeat once again combined chloroform layer, by no
After dehydration over anhydrous sodium sulfate and concentrated to 0.5mL, spare.
Take 0.2mL test solution was evaporated to a small dish, and the solvent evaporated to dryness, add 4 drops of fuming nitric acid to dissolve the residue, evaporated on a water bath, the residue becomes yellow,
After cooling, a few drops of potassium hydroxide - ethanol solution (100g/L), the purple violaceum, immediately becomes red. Atropine, hyoscyamine and scopolamine are
Have this reaction.
A.3 qualitative TLC
A.3.1 Principle
After atropine and other alkaloids contained in the sample were extracted, separated by thin layer, then the color reagent, compared with the control standards.
A.3.2 Reagents
A.3.2.1 silica gel G plate. thickness 0.3mm ~ 0.5mm, 105 ℃ activation 1h, put desiccator.
A.3.2.2 developing solvent. methanol - ammonia (2003).
A.3.2.3 reagent. Weigh 0.85g times bismuth nitrate added 10mL of glacial acetic acid, add 40mL water, dissolved. Take 5mL, plus 5mL iodide
Potassium solution (4g of potassium iodide was dissolved in 5mL of water), plus 20mL of glacial acetic acid was diluted, with water to 100mL.
A.3.2.4 atropine standard solution. Weigh 120.0mg atropine sulfate, dissolved in 10mL water, add ammonia (11) alkaline, with trichloro
Methane extraction twice, each 8mL, chloroform extract was a little over anhydrous sodium sulfate, filtered into 20mL tube with a stopper colorimetric, and then less
Xu chloroform wash the filter, lotion incorporated into the colorimetric tube, add chloroform to 20mL, this is equivalent to 5.0mg per milliliter solution of atropine.
A.3.2.5 scopolamine standard solution. Weigh 145.0mg scopolamine hydrobromide, dissolved in 10mL water, add ammonia (11) alkaline,
Extracted with chloroform twice, each 8mL, chloroform extract was a little over anhydrous sodium sulfate, filtered into 20mL colorimetric tube with a stopper,
A little chloroform and then wash the filter, lotion incorporated into the colorimetric tube, add chloroform 20mL, dubbed equivalent to 5.0mg per milliliter East buttercup
A.3.3 Analysis step
In the TLC plate at the lower end of 2cm, point 10μL atropine and scopolamine standard solution, 30μL ~ 100μL sample extract concentrate, each
Spacing 1.5cm, previously placed expansion tank with developing solvent saturated until the solvent front exhibition to 10cm ~ 15cm removed, evaporated to dryness Expand
Agents, spray chromogenic agent orange-red spots on a positive reaction.
Appendix B
Ergot test methods
B.1 Identification
B.1.1 morphology
Ergot elongated strip or banana-shaped, sometimes slightly flat, long 3mm ~ 10mm, coarse 1mm ~ 7mm, black or purple outside, there
Longitudinal grooves and transverse cracks, brittle, easily broken, section flat, blunt polygonal or oval, center white, gray or pink and white. Sclerotia germination after dormancy
Hair will produce sub-mount; infertility child seat elongate shank, head flat spherical diameter of 1mm ~ 2mm, red-brown, the outer edge of raw perithecium.
B.1.2 tissue sections
Ergot will soak into the water, soaking 24h, the expansion, caught in the middle to fix potatoes or turnips with a small scalpel cut into small thin as possible
Slices with methylene blue solution (1g/L) color was observed under a microscope, the organization closely.
B.2 ergot alkaloids ergot red pigment and qualitative
B.2.1 Reagents
B.2.1.1 tartaric acid solution (20g/L).
B.2.1.2 anhydrous ether.
B.2.1.3 saturated sodium bicarbonate solution.
B.2.1.4 ammonia (11).
B.2.1.5 chloroform.
B.2.1.6 of dimethylaminobenzaldehyde solution. Weigh 0.125g of dimethylaminobenzaldehyde, plus 100mL dilute sulfuric acid (65mL sulfuric acid slowly
Poured into 35mL of water, mix, cooling) was dissolved, and then adding 0.1mL ferric chloride solution (50g/L), mix.
B.2.1.7 ethanol. wavelength of 365nm UV light under observation without fluorescence.
B.2.2 analysis step
Take 20 suspicious ergot in a mortar, pestle, add tartaric acid solution (20g/L) research into viscous, finely ground with ether three times
10mL, ether layers were combined in a test tube, hold the residue in a mortar. In the test tube 0.5mL saturated sodium bicarbonate solution, shaken, carbonate
Sodium hydroxide solution layer turns red, it means that the detection ergot red.
Take the residue retained, ammonia water (11) Polishing was made basic and extracted three times with chloroform, each 10mL, combined chloroform layers minutes
Into two parts. Carefully add 2mL part of dimethylaminobenzaldehyde solution, leaving it in contact with the liquid layer two blue-violet ring, after a few minutes, trichlorosilane
Methane layer were significantly blue, it means that the detection of ergot alkaloids. Another part of the chloroform extract was placed in a test tube in a hot water bath to make trichlorosilane
Alkyl play to make, and the residue was dissolved plus ethanol, at a wavelength of 365nm UV lamp observed a strong blue fluorescence, which indicates detection of ergot
B.3 formula
Said 1000g (m1) samples after the detection of ergot weighed (m2), accurate to 1g. Ergot content (w) mass fraction (%) expressed by the formula
(B.1) Calculated.
w =
m1 ×
100% (B.1)
w --- sample ergot content,%;
m2 --- ergot sample mass, in grams (g);
m1 --- sample weight in grams (g).
Results to three significant figures.
[1] BYE, SOSAV.MolecularPhylogenyoftheJimsonweedGenusDatura (Solanaceae) [J] .Sys-
tematicBotany, 2013,38 (3). 818-829.
Related standard:   GB 19640-2016  GB 19643-2016
Related PDF sample:   GB 19640-2016  GB 19643-2016
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