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GA/T 1693-2020: Forensic sciences - Specifications for second generation sequencing-based DNA examination
Status: Valid GA/T 1693: Evolution and historical versions
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Forensic sciences - Specifications for second generation sequencing-based DNA examination
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GA/T 1693-2020
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(Forensic Science Seal Inspection Part 4: Measurement Comparison Method)
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Basic data | Standard ID | GA/T 1693-2020 (GA/T1693-2020) | | Description (Translated English) | Forensic sciences - Specifications for second generation sequencing-based DNA examination | | Sector / Industry | Public Security (Police) Industry Standard (Recommended) | | Classification of Chinese Standard | A92 | | Classification of International Standard | 13.310 | | Word Count Estimation | 18,182 | | Date of Issue | 30/10/2020 | | Date of Implementation | 1/1/2021 | | Issuing agency(ies) | Ministry of Public Security | GA/T 1693-2020: Forensic sciences - Specifications for second generation sequencing-based DNA examination
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Forensic Science DNA Second Generation Preliminary Inspection Specification)
ICS 13.310
A92
Public Safety Industry Standards of the People's Republic of China
Forensic Science DNA Second-Generation Sequencing Inspection Specification
2020-10-30 released
2021-01-01 implementation
Issued by the Ministry of Public Security of the People's Republic of China
Table of contents
Foreword Ⅰ
1 Scope 1
2 Normative references 1
3 Terms and definitions 1
4 Abbreviations 3
5 General 3
6 Principle 4
7 Inspection equipment and reagents 4
8 Inspection process 4
9 Data Analysis 5
10 Calculation of genetic parameters 7
Appendix A (informative appendix) Forensic Science DNA Second-Generation Sequencing Test Record Form 8
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying these patents.
This standard was proposed by the Forensic Examination Subcommittee of the National Criminal Technology Standardization Technical Committee (SAC/TC179/SC6) and
Focus.
This standard was drafted by. Material Evidence Appraisal Center of the Ministry of Public Security, Sichuan University, Guangdong Provincial Public Security Department.
The main drafters of this standard. Ye Jian, Ji An'an, Wang Le, Kang Klei, Hou Yiping, Zhang Chi, Li Haiyan, Liu Yihui, Zhang Guangfeng.
Forensic Science DNA Second-Generation Sequencing Inspection Specification
1 Scope
This standard specifies the terms and definitions for the use of next-generation sequencing technology for targeted sequencing testing of human DNA genetic markers in forensic sciences.
Principles, inspection equipment and reagents, inspection procedures, as well as basic requirements for data analysis and calculation of genetic parameters.
This standard is applicable to genetic markers such as STR, SNP, InDel, and mitochondrial DNA of various forensic materials.
Generation sequencing inspection and analysis, providing genetic information for forensic science practice, applicable to all DNA laboratories and products engaged in forensic science inspection
Product manufacturer.
2 Normative references
The following documents are indispensable for the application of this document. For dated reference documents, only the dated version applies to this article
Pieces. For undated reference documents, the latest version (including all amendments) is applicable to this document.
GB/T 27025-2019 General requirements for the competence of testing and calibration laboratories
GA/T 383-2014 Forensic Science DNA Laboratory Inspection Specifications
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
DNA sequencing DNAsequencing
The determination of the nucleotide sequence of DNA molecules, that is, the determination of adenine (A), guanine (G), and cytosine that constitute nucleic acid molecules
(C) and the order of thymine (T).
3.2
Second generation sequencing
Different from traditional Sanger (dideoxy method) sequencing, a technology that can sequence a large number of nucleic acid molecules in parallel at one time, usually one
The sequencing reaction can produce no less than 100Mb of sequencing data.
3.3
Targetedsequencing
Sequencing of known and new variants in specific gene sets or genomic regions can effectively reduce sequencing costs, increase sequencing depth, and improve sequencing.
To effectively study the genetic variation of a specific area.
3.4
Bridge PCR bridgepolymerasechainreaction
The DNA sequences at both ends of the library specifically bind to the primer sequences fixed on the surface of the chip, and the library DNA is used as a template for bridge expansion.
increase. Through the cyclic process of continuous denaturation and amplification, each DNA fragment is replicated in its own position and concentrated into bundles to generate DNA
cluster. Each DNA cluster contains thousands of monoclonal amplicons. Using each single strand of DNA as a template, the complementary strands of DNA are synthesized one by one, and the base
The base signal intensity is amplified to reach the signal intensity required for sequencing.
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