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GA/T 1610-2019: Forensic sciences - Examination methods for erythromycin and roxithromycin in biological samples - LC-MS
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Forensic sciences - Examination methods for erythromycin and roxithromycin in biological samples - LC-MS
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GA/T 1610-2019
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Basic data | Standard ID | GA/T 1610-2019 (GA/T1610-2019) | | Description (Translated English) | Forensic sciences - Examination methods for erythromycin and roxithromycin in biological samples - LC-MS | | Sector / Industry | Public Security (Police) Industry Standard (Recommended) | | Classification of Chinese Standard | A92 | | Classification of International Standard | 13.310 | | Word Count Estimation | 14,149 | | Date of Issue | 2019 | | Date of Implementation | 2019-12-01 | | Issuing agency(ies) | Ministry of Public Security | GA/T 1610-2019: Forensic sciences - Examination methods for erythromycin and roxithromycin in biological samples - LC-MS
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Forensic sciences - Examination methods for erythromycin and roxithromycin in biological samples - LC-MS
ICS 13.310
A92
Public Safety Industry Standard of the People's Republic of China
Erythromycin and forensic biological samples
Roxithromycin inspection liquid chromatography-mass spectrometry
Published on October 14,.2019
2019-12-01 Implementation
Issued by the Ministry of Public Security of the People's Republic of China
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
Please note that some content of this document may involve patents. The issuer of this document does not assume responsibility for identifying these patents.
This standard was proposed by the National Toxic Analysis Sub-Committee of Criminal Technology Standardization Technical Committee (SAC/TC179/SC1) and
Be in charge.
This standard was drafted by. Physical Evidence Appraisal Center of the Ministry of Public Security, Shenzhen Public Security Bureau.
The main drafters of this standard. Zhang Yunfeng, Wang Fanglin, Huang Jian, Wang Jiong, Chang Jing, Wang Ruihua, Dong Ying, Zhang Leiping, Hou Xiaoping, Luan Yujing, Du Hongyan,
Cui Guanfeng, Yu Zhongshan, He Yi, Li Shuhui.
Erythromycin and forensic biological samples
Roxithromycin inspection liquid chromatography-mass spectrometry
1 Scope
This standard specifies the liquid chromatography of erythromycin and roxithromycin in forensic scientific biological materials (blood, urine, liver, kidney, stomach and stomach contents, etc.)-
Mass spectrometry (LC-MS) qualitative and quantitative test method.
This standard applies to qualitative and quantitative analysis of erythromycin and roxithromycin in forensic biological samples. Red in other suspicious samples
The qualitative analysis and quantitative analysis of amycin and roxithromycin can be used by reference.
2 Normative references
The following documents are essential for the application of this document. For dated reference documents, only the dated version applies to this article
Pieces. For the cited documents without date, the latest version (including all amendments) applies to this document.
GB/T 6682 Analysis of laboratory water specifications and test methods
GA/T 122 Nomenclature of poison analysis
3 Terms and definitions
The terms and definitions defined in GA/T 122 apply to this document.
4 Principle
Using blank samples and added samples as controls, according to the requirements of parallel operation, extract, purify and concentrate biological samples, using liquid color
Spectral-mass spectrometric quantification, based on retention time, mass spectrometry ion and relative abundance ratio as qualitative judgment basis; based on peak area,
Quantitative analysis was carried out by external standard method.
5 Reagents and materials
5.1 Reagents
The experimental water shall comply with the first-grade water specified in GB/T 6682.The reagents used in the analysis are all chromatographically pure.The reagents include.
a) methanol;
b) acetonitrile;
c) formic acid;
d) ammonia water;
e) 0.1% formic acid in 5mmol/L ammonium formate aqueous solution. Weigh 0.315g of ammonium formate and dissolve with water, add 1mL of formic acid and dilute with water
To 1000mL;
f) 0.1mol/L phosphate buffer (pH value 8). Weigh 5.59g of dipotassium hydrogen phosphate and 0.41g of potassium dihydrogen phosphate, and dilute with water to
1000mL;
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