YC/T 468-2021 English PDF

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YC/T 468-2021: Tobacco and tobacco products - Determination of total alkaloids - Continuous flow (potassium thiocyanate) method
Status: Valid

YC/T 468: Historical versions

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
YC/T 468-2021	279	Add to Cart	3 days	Tobacco and tobacco products - Determination of total alkaloids - Continuous flow (potassium thiocyanate) method	Valid
YC/T 468-2013	319	Add to Cart	3 days	Tobacco and tobacco products. Determination of total alkaloids. Continuous flow(potassium thiocyanate)method	Obsolete

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YC/T 405.5   GB 5606.2   GB/T 5606.1   YC/T 472   YC/T 475   YC/T 471   

Basic data

Standard ID: YC/T 468-2021 (YC/T468-2021)
Description (Translated English): Tobacco and tobacco products - Determination of total alkaloids - Continuous flow (potassium thiocyanate) method
Sector / Industry: Tobacco Industry Standard (Recommended)
Classification of Chinese Standard: X87
Word Count Estimation: 12,154
Issuing agency(ies): State Tobacco Monopoly Administration

YC/T 468-2021: Tobacco and tobacco products - Determination of total alkaloids - Continuous flow (potassium thiocyanate) method

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Tobacco and tobacco products - Determination of total alkaloids - Continuous flow (potassium thiocyanate) method ICS 65.160 CCSX87 People's Republic of China Tobacco Industry Standards Replaces YC/T 468-2013 Determination of total plant alkaloids in tobacco and tobacco products Continuous flow (potassium thiocyanate) method Published on 2021-12-03 2022-03-01 Implementation Released by the State Tobacco Monopoly Administration

foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" drafted. This document replaces YC/T 468-2013 "Continuous Flow (Potassium Thiocyanate) Method for the Determination of Total Phytoalkaloids in Tobacco and Tobacco Products". Compared with YC/T 468-2013, except for editorial changes, the main technical changes are as follows. --- Define the scope of application of the standard as "tobacco and tobacco products (tobacco leaves, tobacco stems, reconstituted tobacco leaves and tobacco mixtures)" (see Chapter 1); --- Added 5% acetic acid solution (see 5.3); --- Increased system flushing fluid (see 5.4); --- Modified buffer solution A (see 5.7, 4.10 of the.2013 edition); --- Modified buffer solution B (see 5.8, 4.11 of the.2013 edition); --- Modified the flow chart of the continuous flow analyzer for total phytoalkaloids (see Appendix A); --- Increased repeatability and reproducibility (see Appendix B). This document uses the redrafted method to modify the use of ISO 22980.2020 "Determination of the total phytoalkaloid content of tobacco (as nicotine) in continuous flow KSCN/DCIC Law. Compared with ISO 22980.2020, this document has many adjustments in structure, and the comparison list of chapter number changes between the two documents See Appendix C. Compared with ISO 22980.2020, there are technical differences in this document. In the outer margin of the clauses involved, a vertical single Lines (|) are marked, and a list of these technical differences and their reasons is given in Appendix D. Please note that some content of this document may be patented. The issuing agency of this document assumes no responsibility for identifying patents. This document is proposed by the State Tobacco Monopoly Administration. This document is under the jurisdiction of National Tobacco Standardization Technical Committee Cigarette Sub-Technical Committee (SAC/TC144/SC1). This document is drafted by. National Tobacco Quality Supervision and Inspection Center, Shanghai Tobacco Group Beijing Cigarette Factory Co., Ltd., China Tobacco Standard Chemical Research Center, Jiangsu China Tobacco Industry Co., Ltd., Zhengzhou Tobacco Research Institute, Yunnan China Tobacco Industry Co., Ltd., Guizhou China Tobacco Industry Co., Ltd., Northwest Tobacco Quality Supervision and Testing Station, Shandong China Tobacco Industry Co., Ltd., Henan Tobacco Quality Supervision and Testing Station, Cloud Nanzhong Tobacco Reconstituted Tobacco Co., Ltd., Yunnan Tobacco Quality Supervision and Testing Station, and Sichuan Tobacco Quality Supervision and Testing Station. The main drafters of this document. Zhang Wei, Liu Nan, Ma Yanjun, Zhou Jun, He Shengbao, Ding Li, Feng Xiaomin, Xu Ruyan, Luo Anna, Wang Yingyuan, Du Guorong, Peng Qianrong, Peng Lijuan, Gong Zhenlin, Cheng Tao, Zhang Haiyan, Chen Zhihao, Ma Li, Liu Jian, Liu Enfen, Li Ling, Faye Wong, Liu Wei, Wang Kai, Guo Junwei, Shi Hongyan, Zhang Jie, Wang Chunqiong, Qin Yunhua, Zhang Chunhui, Liao Huiyun, Wang Hongbo, Shao Jimin. The previous versions of this document and its superseded documents are as follows. ---YC/T 468-2013; ---This is the first revision. Determination of total plant alkaloids in tobacco and tobacco products Continuous flow (potassium thiocyanate) method

1 Scope

This document specifies the continuous determination of total plant alkaloids (as nicotine) in tobacco and tobacco products (tobacco leaves, stems, reconstituted tobacco leaves and tobacco blends) Flow (potassium thiocyanate) determination method. This document applies to the determination of total plant alkaloids (as nicotine) in tobacco and tobacco products (tobacco leaves, stems, reconstituted tobacco leaves and tobacco blends) Determination.

2 Normative references

The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, dated citations documents, only the version corresponding to that date applies to this document; for undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analysis Laboratory Water Specifications and Test Methods YC/T 31 Preparation of Tobacco and Tobacco Products Samples and Moisture Determination Oven Method YC/T 247 Determination of Nicotine Purity of Tobacco and Tobacco Products—Silicon Tungstic Acid Gravimetric Method

3 Terms and Definitions

There are no terms and definitions that need to be defined in this document.

4 Principles

Tobacco samples were extracted with water, and the total phytoalkaloids (calculated as nicotine) in the extract were reacted with p-aminobenzenesulfonic acid and cyanogen chloride, which was composed of thiocyanate Potassium acid and sodium dichloroisocyanurate are produced online by reaction. The reaction product was measured with a colorimeter at 460 nm. Note. The same results can be obtained with 5% acetic acid solution as the extraction solution. If total plant alkaloids (as nicotine) and reducing sugars (see YC/T 159-2019), 5% acetic acid extract should be used.

5 Reagents

Unless otherwise specified, only analytically pure and above reagents and first-grade water in accordance with GB/T 6682 are used in the analysis. 5.1 Reference material Nicotine or nicotine ditartaric acid dihydrate [C10H14N2(C4H6O6)2·2H2O], check nicotine or ditartaric acid dihydrate according to YC/T 247 The purity of nicotine. 5.2 Polyethoxylauryl ether solution (Brij35 solution) 250g of polyethoxylauryl ether [(C2H4O)nC12H26O] was added to 1000mL of water, heated and stirred until dissolved. ......

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