SN/T 3567.1-2013 English PDFUS$319.00 ยท In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 3567.1-2013: Detection method of crossing priming isothermal amplification. Part 1: General technical regulation Status: Valid
Basic dataStandard ID: SN/T 3567.1-2013 (SN/T3567.1-2013)Description (Translated English): Detection method of crossing priming isothermal amplification. Part 1: General technical regulation Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: C62 Classification of International Standard: 13.020 Word Count Estimation: 12,190 Quoted Standard: GB 19489; GB/T 6682 Regulation (derived from): AQSIQ notification issued in 2013 on the first batch of 179 entry-exit inspection and quarantine of industry standards; industry standard for filing Notice 2013 No. 9 (No. 165 overall) Issuing agency(ies): General Administration of Customs Summary: This standard specifies the cross primer isothermal amplification primer design, materials and equipment needed to build the technology platform when amplified and amplified product detection system to establish the specific content, and practices of each SN/T 3567.1-2013: Detection method of crossing priming isothermal amplification. Part 1: General technical regulation---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Detection method of crossing priming isothermal amplification.Part 1. General technical regulation People's Republic of China Entry - Exit Inspection and Quarantine Industry Standard Detection Method of Cross Primer Constant Temperature Amplification Part 1. General technical regulations Part 1. Generaltechnicalregulation 2013-03-01 release 2013-09-16 implementation People's Republic of China State Administration of Quality Supervision, Inspection and Quarantine issued ForewordThis section is part 1 of SN/T 3567. This part is drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuer of this document does not assume responsibility for the identification of these patents. This part of the State Certification and Accreditation Administration Committee proposed and centralized. This part of the drafting unit. the People's Republic of China Tianjin Entry - Exit Inspection and Quarantine Bureau. This part of the main drafters. Qi Jun, Zhang Xia, Wang Xin, Liu Zhenyu, Zuo Feng, Chai Hongsen, Zhan Xijing, Wei Yongxin, Li Zhihui, especially special, Cui Jingbo. Detection Method of Cross Primer Constant Temperature Amplification Part 1. General technical regulations1 ScopeThis part of SN/T 3567 specifies the primer design, materials and instruments required for cross-primers for constant temperature amplification technology. System construction and amplification of product testing specific content, as well as the steps of the operating norms. This part applies to the inspection and quarantine institutions to establish cross-primers constant temperature amplification technology, and based on the technology used in harmful and pathogenic microbes Rapid identification and detection of the material.2 normative reference documentsThe following documents are indispensable for the application of this document. For dated references, dated only dates apply to this file. For undated references, the latest edition (including all modifications) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods GB 19489 General requirements for laboratory biosecurity3 terms and definitionsThe following terms and definitions apply to this document. 3.1 Constant temperature amplification technology isothermalamplificationtechnology A class of nucleic acid amplification method, the whole process of its amplification reaction in a single temperature, without the need for specialized amplification equipment under. With polymerase chain (Polymerasechainreaction, PCR) is different, it does not need to go through dozens of temperature changes in the cycle of the process. 3.2 Cross - primers thermostat amplification technology crossprimingisothermalamplification; CPA A nucleic acid thermostat amplification technique, in addition to a DNA polymerase (DNA) containing a chain displacement function in a CPA amplification system polymerase), but also mainly cross-primers, stripping primers and detection primers. These oligonucleotide chains can rely on the DNA polymerisation Enzyme high activity of the chain displacement characteristics, so that deoxyribonucleic acid (deoxyribonucleicacid, DNA) can continue to achieve the cycle of amplification. 3.3 Cross primers crossprimer The main primers used for cross-amplification, where the 5 'end sequence of the forward primer is identical to the hybridization sequence of the reverse primer, whereas the reverse primer The 5 & apos; end sequence is identical to the hybridization sequence of the forward primer, so that the two primers are introduced into the hybrid sequence to each other during the amplification process, Increase the hybridization site of the primer, and promote the amplification reaction. 3.4 Peel the primer bomperprimer A short chain primer located at the rear of the cross-amplified primer, which serves to extend the amplification primer by the action of strand displacement DNA polymerase The chain is stripped from the template. ...... |
