SN/T 2271-2009 English PDFUS$189.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 2271-2009: Method of the ploymerase chain reaction for detecting genetically modified components in pimiento Status: Valid
Basic dataStandard ID: SN/T 2271-2009 (SN/T2271-2009)Description (Translated English): Method of the ploymerase chain reaction for detecting genetically modified components in pimiento Sector / Industry: Commodity Inspection Standard (Recommended) Classification of Chinese Standard: X26 Classification of International Standard: 67.080 Word Count Estimation: 7,744 Date of Issue: 2009-02-20 Date of Implementation: 2009-09-01 Quoted Standard: SN/T 1193; SN/T 1204 Regulation (derived from): National Quality Inspection [2009] No. 67 Issuing agency(ies): General Administration of Customs Summary: This standard specifies the exogenous gene transgenic peppers CaMV 35S, NPT ��, NOS, CMV Qualitative PCR detection methods. This standard applies to transgenic pepper sample screening test for resistance to cucumber mosaic virus in transgenic pepper identification. SN/T 2271-2009: Method of the ploymerase chain reaction for detecting genetically modified components in pimiento---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order. Method of the ploymerase chain reaction for detecting genetically modified components in pimiento Exit inspection and quarantine industry standard book People's Republic of China Qualitative PCR Detection of Genetically Modified peppers Posted 2009-02-20 2009-09-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released ForewordThis standard is proposed and managed by the National Certification and Accreditation Committee. This standard was drafted. People's Republic of China Shandong CIQ. The main drafters of this standard. Gao Hongwei, Liang beads, with Liu, Wang Yan, YU Li Xin, Cao Zhong Lei. This standard is the first release of the entry-exit inspection and quarantine industry standards. Qualitative PCR Detection of Genetically Modified peppers1 ScopeThis standard specifies the transgenic pepper exogenous gene CaMV35S, NPTⅡ, NOS, CMV qualitative PCR detection method. This standard applies to transgenic pepper sample screening test for the identification of anti-CMV gene transfer of green pepper.2 Normative referencesThe following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard. SN/T 1193 genetic testing laboratory technical requirements SN/T 1204 real-time PCR detection method for genetically modified plants and their processed products 3 Terms, definitions and abbreviations The following terms, definitions and abbreviations apply to this standard. 3.1 Terms and Definitions 3.1.1 This species does not have the will, from other species of functional DNA sequences, by introducing a variety of means to carry out the species Expression, in order for the species to obtain new varieties of features. 3.1.2 Gene sequence template first denatured at high temperatures into a single strand, DNA polymerase and under appropriate reaction conditions, depending on the template sequence Two primers were designed in a period of two complementary sequence of the template DNA strand annealing occurs in conjunction with each other, followed by DNA polymerase Under catalysis in four deoxyribonucleotides (dNTP) as substrates, primer can be extended, and then repeated denaturation, annealing and extension of this A cycle in which the amplified gene fragment For amplification was geometrically. 3.2 Acronyms 3.2.1 Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit gene from a plant chloroplast genome encodes. 3.2.2 Cauliflower mosaic virus 35S promoter. 3.2.3 Neomycin 3'-phosphotransferase. 3.2.4 Nopaline synthase 3 'transcription terminator. ......Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 2271-2009_English be delivered?Answer: Upon your order, we will start to translate SN/T 2271-2009_English as soon as possible, and keep you informed of the progress. 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