GB/T 40194-2021 English PDF

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GB/T 40194-2021: Detection and identification of Barley stripe mosaic virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 40194-2021	259	Add to Cart	3 days	Detection and identification of Barley stripe mosaic virus	Valid

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Basic data

Standard ID: GB/T 40194-2021 (GB/T40194-2021)
Description (Translated English): Detection and identification of Barley stripe mosaic virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Word Count Estimation: 14,135
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 40194-2021: Detection and identification of Barley stripe mosaic virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Barley stripe mosaic virus ICS 65.020.01 CCSB16 National Standards of People's Republic of China Barley Stripe Mosaic Virus Quarantine and Identification Method Released on 2021-05-21 2021-12-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents. This document was proposed and managed by the National Plant Quarantine Standardization Technical Committee (SAC/TC271). Drafting organizations of this document. Chinese Academy of Inspection and Quarantine, Fuzhou Customs of the People's Republic of China. The main drafters of this document. Zhang Yongjiang, Shen Jianguo, Xin Yanyan. Barley Stripe Mosaic Virus Quarantine and Identification Method

1 Scope

This document specifies the serological and molecular biological quarantine and identification methods for barley stripe mosaic virus. This document is applicable to the quarantine and identification of plants and their products that may carry barley stripe mosaic virus.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to This document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Terms and definitions

There are no terms and definitions that need to be defined in this document.

4 Basic information of barley stripe mosaic virus

Scientific name. Barleystripemosaicvirus Abbreviation. BSMV Synonyms. Barleyfalsestripevirus, Barley mildstripemosaicvirus, Barley mildstripevirus, Barleymosaicvirus, Barleystripemosaichordeivirus, Barleyverymildstripemosaicvirus, Oat stripemosaicvirus, Sepipemosaicvirus Taxonomic status. Martellivirales (Martellivirales) Plant Baculoviridae (Virgaviridae) Hordeivirus (Hordei- virus) members. See Appendix A for additional information on barley streak mosaic virus.

5 Principles of the method

The serological and genomic characteristics of barley stripe mosaic virus are the basis for quarantine and identification of the virus. According to the BSMV and antibody The specific reaction between the plant samples and the double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA); based on the genome of BSMV The characteristics are detected by RT-PCR and real-time fluorescent RT-PCR; through the effective combination of these methods, it is determined whether the sample contains BSMV.

6 Instruments and reagents

6.1 Equipment 6.1.1 Electronic balance (with a sense of 0.001g). 6.1.2 High-speed refrigerated centrifuge. ......

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