GB/T 40174-2021 English PDF

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GB/T 40174-2021: Purity detection method of reagent enzymes
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 40174-2021	189	Add to Cart	3 days	Purity detection method of reagent enzymes	Valid

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Basic data

Standard ID: GB/T 40174-2021 (GB/T40174-2021)
Description (Translated English): Purity detection method of reagent enzymes
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: C27
Word Count Estimation: 10,156
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 40174-2021: Purity detection method of reagent enzymes

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Purity detection method of reagent enzymes ICS 07.080 CCSC27 National Standards of People's Republic of China Detection method of tool enzyme purity Released on 2021-05-21 2021-12-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents. This document was proposed and managed by the National Tool Enzyme Standardization Working Group (SAC/SWG11). Drafting organizations of this document. Fujian Nansheng Technology Co., Ltd., Xiahe (Shenzhen) Biotechnology Co., Ltd., Suzhou Kunqi Biotechnology Co., Ltd. Company, Shanghai Yingzi Life Technology Co., Ltd., Leigu (Xiamen) Biomedical Co., Ltd., Fujian Huacan Pharmaceutical Co., Ltd., Xiamen University, Angel Yeast Co., Ltd., Shandong University, Beijing University of Chemical Technology, Fudan University, Institute of Microbiology, Chinese Academy of Sciences, Shenzhen Huayinkangji Because Technology Co., Ltd. The main drafters of this document. Huang Facan, Zhan Xuexiong, Zheng Dengzhong, Zhao Jing, Zhu Li, Zhong Jiang, Zhang Yongyou, Yao Juan, Chen Xiulan, Chen Jinchun, Liu Wenjun, Guo Yanwei. Detection method of tool enzyme purity

1 Scope

This document describes the terms and definitions, principles, reagents or materials, equipment, sample preparation, and test procedures of the tool enzyme purity detection method. Steps, result calculation and quality control. This document is suitable for testing the purity of tool enzymes.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to This document. GB/T 6682 Analytical laboratory water specifications and test methods YY/T 0087 Electrophoresis device

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 Purityofreagentenzymes The mass percentage of the target tool enzyme in the total protein or solid content of the sample. 3.2 Contentofprotein The mass of the protein per milligram or milliliter of the sample was determined by the Folin method. 3.3 Contentofsolid The mass per milligram or milliliter of the anhydrous solid matter obtained after drying the sample.

4 Principle

With the bovine serum albumin standard as a control, the sample was taped after sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Scan the surface, and then calculate the gray value to obtain the quality of the target tool enzyme in the sample, and then use the Folin method to determine the quality of the total protein of the sample Or use the ignition residue inspection method to determine the solid content of the sample to calculate the purity of the tool enzyme.

5 Reagents or materials

Unless otherwise specified, the reagents used are all analytically pure, and the water is secondary water specified in GB/T 6682. 5.1 Alkaline copper solution Weigh 10g of sodium hydroxide, 50g of sodium carbonate, add 400mL of water to dissolve, as a liquid; take 0.5g of potassium tartrate, add 50mL of water to make ......

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