GB/T 33127-2016 English PDF

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GB/T 33127-2016: Detection and identification of Sugarcane streak virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 33127-2016	279	Add to Cart	3 days	Detection and identification of Sugarcane streak virus	Valid

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Basic data

Standard ID: GB/T 33127-2016 (GB/T33127-2016)
Description (Translated English): Detection and identification of Sugarcane streak virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,196
Date of Issue: 2016-10-13
Date of Implementation: 2017-05-01
Regulation (derived from): National Standard Notice No.1716 of 2016
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 33127-2016: Detection and identification of Sugarcane streak virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Sugarcane streak virus ICS 65.020.01 B16 National Standards of People's Republic of China Identification method of sugarcane line virus 2016-10-13 released 2017-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Management Committee released

Foreword

This standard is drafted in accordance with the rules given in GB/T 1.1-2009. This standard is proposed by the National Commission for Standardization of Plant Quarantine Standardization (SAC/TC271). The drafting unit of this standard. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, the People's Republic of China Hainan Entry-Exit Inspection Epidemic situation. The main drafters of this standard. Feng Lixia, Wu Zhentao, Liu Fuxiu, Zhao Lirong, Wang Weifang, Yang Lei Liang. Identification method of sugarcane line virus

1 Scope

This standard specifies the method of quarantine identification of sugarcane line virus. This standard applies to quarantine identification of living host plants that may carry sugarcane line virus.

2 normative reference documents

The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article Pieces. For undated references, the latest edition (including all modifications) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods SN/T 1840 Plant virus immunoelectron microscopy SN/T 2122 Quarantine sampling of inbound and outbound plants and plant products

3 sugar cane line virus basic information

Chinese name. sugar cane line virus. Scientific name. Sugarcanestreakvirus. Abbreviations. SSV. Taxonomic status. Geminiviridae, Mastrevirus. See Appendix A for additional information on sugarcane line viruses.

4 Principle of the method

SSV immunogenicity and genomic characteristics are the basis for the identification of the virus. Using conventional PCR, real-time PCR, double antibody Sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and immunoelectron microscopy were used to detect the samples. 5 Instruments, facilities, utensils and reagents 5.1 Instruments Electronic balance (inductance 0.001g), high-speed centrifuge, small instant centrifuge, ultra-low temperature refrigerator (-80 ℃), ice machine, vortex oscillation , Magnetic stirrer, autoclave, pH meter, transmission electron microscope, clean bench, PCR instrument, real-time fluorescence quantitative PCR instrument, micro Furnace, electrophoresis, electrophoresis tank, gel imaging analyzer, enzyme detector. 5.2 facilities Isolated greenhouse (10 ℃ ~ 30 ℃). 5.3 appliances (2.5 μL, 10 μL, 20 μL, 100 μL, 1000 μL) and an adjustable pipette head, centrifuge tube, mortar, miniature ......

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