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GB/T 33115-2016 English PDF

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GB/T 33115-2016: Detection and identification of Narcissus late season yellows virus
Status: Valid
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GB/T 33115-2016279 Add to Cart 3 days Detection and identification of Narcissus late season yellows virus Valid

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Basic data

Standard ID: GB/T 33115-2016 (GB/T33115-2016)
Description (Translated English): Detection and identification of Narcissus late season yellows virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,112
Date of Issue: 2016-10-13
Date of Implementation: 2017-05-01
Regulation (derived from): National Standard Notice No.1716 of 2016
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 33115-2016: Detection and identification of Narcissus late season yellows virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Narcissus late season yellows virus ICS 65.020.01 B16 National Standards of People's Republic of China Quarantine of Narcissus in Late Season 2016-10-13 released 2017-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Management Committee released

Foreword

This standard is drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The release of this document The Agency does not assume responsibility for identifying these patents. This standard is proposed by the National Commission for Standardization of Plant Quarantine Standardization (SAC/TC271). The drafting unit of this standard. Fujian Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, China Inspection and Quarantine Science Research Institute, the Chinese People's Republic of China And Ningbo International Entry and Exit Inspection and Quarantine Bureau, Fujian Agriculture and Forestry University. The main drafters of this standard. Shen Jianguo, Li Min, Cai Wei, Li Guifen, Zhang Yongjiang, Yu Yun, Yu Wentao, Guo Lixin, Gao Fangluan, Wu Zujian. Quarantine of Narcissus in Late Season

1 Scope

This standard specifies the quarantine identification method for Narcissuslateseasonyellows virus. This standard is applicable to quarantine identification of narcissus and narcissus products that may carry daffodil yellowing virus.

2 normative reference documents

The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article Pieces. For undated references, the latest edition (including all modifications) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods SN/T 2122 Quarantine sampling of inbound and outbound plants and plant products Code for the detection of plant viruses Code for molecular biology detection of plant viruses

3 narcissus late season yellow virus basic information

Chinese name. Narcissus late season yellow virus. Scientific name. Narcissuslateseasonyellowsvirus, abbreviation. NLSYV. Taxonomic status. Potato virus (Potyviridae), Potato virus (Potyvirus). For additional information on daffodil yellowing virus, see Appendix A.

4 Principle of the method

The serological and molecular biological characteristics of the daffodil virus were the main basis for the development of the quarantine identification method. 5 instruments, utensils and reagents 5.1 Instruments High-speed refrigerated centrifuge, electronic balance, PCR instrument, fluorescence PCR instrument, horizontal electrophoresis system, -20 ℃ low temperature refrigerator and -80 ℃ ultra low Temperature refrigerator, gel imaging analysis system, pH meter, microwave oven, magnetic stirrer, constant temperature water bath, autoclave, clean bench, ELISA Instrument and so on. 5.2 Appliances (2.5 μL, 10 μL, 20 μL, 100 μL,.200 μL, 1000 μL) and an adjustable pipette head, centrifuge tube, Bowl and so on. 5.3 Reagents Unless otherwise specified, all reagents are of analytical grade and the test water shall comply with the relevant provisions in GB/T 6682. PTA-ELISA assay
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