GB/T 33114-2016 English PDF

US$279.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB/T 33114-2016: Detection and identification of Prunus necrotic ringspot virus
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB/T 33114-2016	279	Add to Cart	3 days	Detection and identification of Prunus necrotic ringspot virus	Valid

Similar standards

GB/T 43165   GB/T 40135   GB/T 19495.4   GB/T 33116   GB/T 33115   

Basic data

Standard ID: GB/T 33114-2016 (GB/T33114-2016)
Description (Translated English): Detection and identification of Prunus necrotic ringspot virus
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Classification of International Standard: 65.020.01
Word Count Estimation: 14,140
Date of Issue: 2016-10-13
Date of Implementation: 2017-05-01
Regulation (derived from): National Standard Notice No.1716 of 2016
Issuing agency(ies): General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China, Standardization Administration of the People's Republic of China

GB/T 33114-2016: Detection and identification of Prunus necrotic ringspot virus

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Prunus necrotic ringspot virus ICS 65.020.01 B16 National Standards of People's Republic of China Identification method of necrotic ring spot virus 2016-10-13 released 2017-05-01 implementation General Administration of Quality Supervision, Inspection and Quarantine of the People's Republic of China China National Standardization Management Committee released

Foreword

This standard is drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The release of this document The Agency does not assume responsibility for identifying these patents. This standard is proposed by the National Commission for Standardization of Plant Quarantine Standardization (SAC/TC271). The drafting of this standard. China Inspection and Quarantine Science Research Institute, the People's Republic of China Hunan Entry-Exit Inspection and Quarantine Bureau, Fujian Province Agriculture Academy. The main drafters of this standard. Zhang Yongjiang, Huang Yingbo, Kong Jun, Xie Lixue, Li Guifen, Ma Jie, Xin Yizhen, Wei Meisheng. Identification method of necrotic ring spot virus

1 Scope

This standard specifies the method of quarantine identification of Prunusnecroticringspotvirus. This standard applies to quarantine identification of plants and their products that may carry the genus Nematocysts.

2 normative reference documents

The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article Pieces. For undated references, the latest edition (including all modifications) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods SN/T 2122 Quarantine sampling of inbound and outbound plants and plant products Code for the detection of plant viruses Code for molecular biology detection of plant viruses

3 is a necrotic ring spot virus basic information

Scientific name. Prunusnecroticringspotvirus Abbreviations. PNRSV Taxonomic status. Bromoviridae, Ilarvirus. For other information, see Appendix A.

4 Principle of the method

The serological and genomic characteristics of the necrotic ring spot virus are the basis for the identification of the virus. According to PNRSV with antibodies (DAS-ELISA); the gene based on PNRSV was used to isolate the plant samples by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) Group of RT-PCR, real-time fluorescence RT-PCR detection method; through the effective combination of these methods to determine whether the sample with PNRSV. 5 instruments, utensils and reagents 5.1 Instruments Electronic balance (inductance 0.001g), high-speed refrigerated centrifuge, small instant centrifuge, ordinary refrigerator, ultra-low temperature refrigerator (-80 ℃), the system Ice machine, vortex oscillator, magnetic stirrer, autoclave, pH meter, clean bench, PCR instrument, microwave oven, electrophoresis, electrophoresis tank, gel Imaging analyzer, microplate reader. 5.2 Appliances (2.5 μL, 10 μL, 20 μL, 100 μL,.200 μL, 1000 μL) and an adjustable pipette head, Eppendorf Tube, mortar, micro-grinding pestle and so on. ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB/T 33114-2016_English be delivered?

Answer: Upon your order, we will start to translate GB/T 33114-2016_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB/T 33114-2016_English with my colleagues?

Answer: Yes. The purchased PDF of GB/T 33114-2016_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.