GB 1903.79-2025 English PDF

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GB 1903.79-2025: National food safety standard - Nutrition fortifier - L-Lysine-L-aspartate
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB 1903.79-2025	179	Add to Cart	3 days	National food safety standard - Nutrition fortifier - L-Lysine-L-aspartate	Valid

Basic data

Standard ID: GB 1903.79-2025 (GB1903.79-2025)
Description (Translated English): National food safety standard - Nutrition fortifier - L-Lysine-L-aspartate
Sector / Industry: National Standard
Classification of Chinese Standard: X40
Word Count Estimation: 9,927
Date of Issue: 2025-09-02
Issuing agency(ies): National Health Commission; State Administration for Market Regulation

GB 1903.79-2025: National food safety standard - Nutrition fortifier - L-Lysine-L-aspartate

---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB1903.79-2025
National Standards of the People's Republic of China National Food Safety Standards L-Lysine, a food fortifier, is also known as L-Lysine (or L-ascorbic acid aspartate Published on 2025-09-02 Implemented on 2026-03-02 National Health Commission of the People's Republic of China State Administration for Market Regulation issued National Food Safety Standards L-Lysine, a food fortifier, is also known as L-Lysine (or L-ascorbic acid aspartate 1.Scope This standard applies to products made from starch or sugars via fermentation; or products made from L-lysine (or L-lysine salt) and L-aspartate amino acids. L-Lysine and L-aspartate salts are food fortifiers produced by chemical synthesis from aspartic acid. 2.Chemical name, molecular formula, structural formula, and relative molecular mass 2.1 Chemical Name L-2,6-Diaminohexanoic acid L-2-aminosuccinate 2.2 Molecular Formula C10H21N3O6 2.3 Structural Formula 2.4 Relative Molecular Mass 279.29 (based on 2022 international relative atomic mass)

3 Technical Requirements

3.1 Sensory Requirements Sensory requirements shall comply with the provisions of Table 1. Table 1 Sensory Requirements Project Requirements Inspection Methods White Crystalline or crystalline powder Place an appropriate amount of sample in a clean, dry white porcelain dish and observe it under natural light. Observe its color and condition 3.2 Physicochemical Indicators The physicochemical properties should meet the requirements of Table 2. Table 2 Physicochemical Indicators Project indicator testing methods L-Lysine and L-aspartate content (on a dry basis), w/% ≥ 98.0 (See Appendix A, A.4) Specific rotation αm(20℃, D)/[(°)·dm2·kg-1] 24.0~26.5 (See Appendix A, A.5) pH (50 g/L aqueous solution). 5.0~7.0 (Appendix A, A.6) Loss on drying, w/% ≤ 0.2 (Appendix A, A.7) Residue on ignition, w/% ≤ 0.1 (Appendix A, A.8) Chloride (as Cl), w/% ≤ 0.04 (Appendix A, A.9) Lead (Pb)/(mg/kg) ≤ 0.3 GB 5009.12 or GB 5009.75 Total arsenic (as As)/(mg/kg) ≤ 0.2 GB 5009.11 or GB 5009.76

Appendix A

Test methods A.1 Safety Warning Some reagents used in this test method are toxic and corrosive; therefore, care must be taken during handling. If splashed on skin, rinse immediately with water. Rinse thoroughly; severe cases require immediate treatment. When using flammable materials, never heat with an open flame. A.2 General Provisions Unless otherwise specified in this standard, the reagents used shall be of analytical grade, and the standard titration solutions, impurity determination standard solutions, preparations, and reagents used shall be of analytical grade. The products shall be prepared in accordance with the provisions of GB/T 601, GB/T 602, and GB/T 603 respectively, and the test water shall meet the requirements of Grade III water in GB/T 6682. Unless otherwise specified, all solutions used in the experiment are aqueous solutions. A.3 Identification Test Infrared identification. The determination of infrared spectroscopy analysis shall be performed in accordance with 6.2 of GB/T 32199 or GB/T 6040.The spectrum of the sample shall be compared with that of the standard. Comparing the figures (see Figure B.1 in Appendix B), the two should be basically consistent. A.4 Determination of L-L-Lysine and L-Aspartate (on a dry basis) A.4.1 Method Summary The sample was titrated with a standard perchloric acid solution using formic acid as a co-solvent and acetic acid as the solvent. The titration was based on the amount of standard perchloric acid solution consumed. The L-lysine and L-aspartate content was calculated by volume as C10H21N3O6. A.4.2 Reagents and Materials A.4.2.1 Formic acid. A.4.2.2 Acetic acid. A.4.2.3 Perchloric acid. A.4.2.4 Crystal Violet. A.4.2.5 Standard perchloric acid titration solution. c(HClO4) = 0.1 mol/L. A.4.2.6 Crystal Violet-Acetic Acid Indicator (5g/L). Weigh 0.5g of crystal violet, dissolve it in acetic acid, and dilute to 100mL with acetic acid. A.4.3 Instruments and Equipment A.4.3.1 Electronic balance with a sensitivity of 0.0001g. A.4.3.2 Potentiometric titrator. A.4.4 Analysis Steps Weigh 0.2 g (accurate to 0.0001 g) of the sample into a beaker or conical flask, add 3 mL of formic acid and 50 mL of acetic acid, and dissolve and mix well. Titrate with standard perchloric acid solution using a potentiometric titrator until the endpoint. Alternatively, add 3 drops of crystal violet-acetic acid indicator; the solution color will change from purple to light purple. The endpoint is when the color changes from blue to green. A blank test is also conducted simultaneously. A.4.5 Result Calculation The mass fraction w1 of L-lysine and L-aspartate (on a dry basis) is calculated according to formula (A.1). w1= (V1-V0)×c×9.310 m×100 × 100-w2× 100% (A.1) In the formula. V1 --- The volume of perchloric acid standard titration solution consumed by the sample solution, in milliliters (mL); V0 --- The volume of perchloric acid standard titration solution consumed by the blank solution, in milliliters (mL); c --- Molar concentration of the perchloric acid standard titration solution, in moles per liter (mol/L); 9.310 --- Each 1 mL of 0.1 mol/L perchloric acid standard titration solution is equivalent to 9.310 mg of L-lysine and L-aspartic acid. Salt (C10H21N3O6); m --- Sample mass, in grams (g); 100 --- Conversion factor; w2 --- Loss on drying of the sample. The calculation results are expressed as the arithmetic mean of two independent measurements obtained under repeatability conditions, and the results are rounded to one decimal place. The absolute difference between two independent measurements obtained under repeatability conditions shall not exceed 2% of the arithmetic mean. A.5 Determination of specific rotation A.5.1 Reagents and Materials A.5.1.1 Hydrochloric acid. 36%~38%. A.5.1.2 Hydrochloric acid solution (6mol/L). Measure 540mL of hydrochloric acid, dilute with water and bring the volume to 1000mL. A.5.2 Instruments and Equipment A.5.2.1 Electronic balance with a sensitivity of 0.0001g. A.5.2.2 Polarimeter. A.5.2.3 Vacuum drying oven (or desiccant). A.5.3 Analysis Steps Weigh 4 g (accurate to 0.0001 g) of the dried sample (according to method A.7), dissolve it in 6 mol/L hydrochloric acid solution, and adjust the solution... Temperature to 20℃, transfer to a 50mL volumetric flask and dilute to volume with 6mol/L hydrochloric acid solution, then mix well. Measure according to the method specified in GB/T 613. Determine and calculate the specific rotation of L-lysine and L-aspartate. A.5.4 Result Calculation The specific rotation αm (20℃, D) of L-lysine and L-aspartate is expressed in units of "(°)·dm2·kg-1", according to the formula. (A.2) Calculate. αm(20℃,D)= l×ρ (A.2) In the formula. α --- Optical rotation as measured by a polarimeter, in degrees (°); l --- the length of the optical polarimeter, in decimeters (dm); ρ --- The mass concentration of L-lysine and L-aspartate in the solution, expressed in grams per milliliter (g/mL). A.6 pH Measurement A.6.1 Instruments and Equipment A.6.1.1 Electronic balance with a sensitivity of 0.01g. ......

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