GB 1903.76-2025 English PDF

US$159.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
GB 1903.76-2025: National food safety standard - Nutrition fortifier - Hematin
Status: Valid

Standard ID	USD	BUY PDF	Lead-Days	Standard Title (Description)	Status
GB 1903.76-2025	159	Add to Cart	3 days	National food safety standard - Nutrition fortifier - Hematin	Valid

Basic data

Standard ID: GB 1903.76-2025 (GB1903.76-2025)
Description (Translated English): National food safety standard - Nutrition fortifier - Hematin
Sector / Industry: National Standard
Classification of Chinese Standard: X40
Word Count Estimation: 8,817
Date of Issue: 2025-09-02
Issuing agency(ies): National Health Commission; State Administration for Market Regulation

GB 1903.76-2025: National food safety standard - Nutrition fortifier - Hematin

---This is an excerpt. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.), auto-downloaded/delivered in 9 seconds, can be purchased online: https://www.ChineseStandard.net/PDF.aspx/GB1903.76-2025
National Standards of the People's Republic of China National Food Safety Standards Food fortifier heme iron Published on 2025-09-02 Implemented on 2026-03-02 National Health Commission of the People's Republic of China State Administration for Market Regulation issued National Food Safety Standards Food fortifier heme iron 1.Scope This standard applies to the use of animal blood that has passed inspection and quarantine or blood cells obtained through centrifugation as raw materials, which undergo enzymatic hydrolysis, separation, and drying. The resulting food fortifier is heme iron. 2.Chemical name, molecular formula, structural formula, and relative molecular mass 2.1 Chemical Name Heme iron 2.2 Molecular Formula C34H32FeN4O4 2.3 Structural Formula 2.4 Relative Molecular Mass 616.499 (based on 2022 international relative atomic mass)

3 Technical Requirements

3.1 Sensory Requirements Sensory requirements shall comply with the provisions of Table 1. Table 1 Sensory Requirements Project Requirements Inspection Methods Color ranges from reddish-brown to dark brown Powder or granules Odorless or with a slight characteristic odor of heme iron Impurities. None visible to the naked eye Take an appropriate amount of sample and place it in a clean, dry, transparent glass container. Observe its color and condition under natural light, smell its odor, and observe its impurities. 3.2 Physicochemical Indicators The physicochemical properties should meet the requirements of Table 2. Table 2 Physicochemical Indicators Project indicator testing methods Iron (as Fe), w/% 1.0~2.6 GB 5009.90 Heme iron (calculated as C34H32FeN4O4), w/% 9.0~27.0 (See Appendix A, A.3) Loss on drying, w/% ≤ 6.0 GB 5009.3 (Direct drying method) Residue on ignition, w/% ≤ 12.0 GB 5009.4 Lead (Pb)/(mg/kg) ≤ 2.0 GB 5009.75 or GB 5009.12 Total arsenic (as As)/(mg/kg) ≤ 2.0 GB 5009.76 or GB 5009.11 3.3 Microbial limits Microbial limits should comply with the provisions of Table 3. Table 3 Microbial Limits project Sampling scheme a and limits ncm M Test methods Salmonella/25g 5 0 0 - GB 4789.4 Staphylococcus aureus/(CFU/g) 5 1 100 1000 GB 4789.10 Method II a. The sampling and processing of samples shall be carried out in accordance with GB 4789.1.

Appendix A

Test methods A.1 General Provisions Unless otherwise specified, the reagents and water used in the test methods of this standard refer to analytical grade reagents and those specified in GB/T 6682. The solution used in the experiment is a grade III water. Unless otherwise specified, all solutions used in the experiment are aqueous solutions. A.2 Identification Test A.2.1 Reagents and Materials A.2.1.1 Sodium hydroxide. A.2.1.2 Pyridine. A.2.1.3 Sodium hyposulfite. A.2.1.4 Nitric acid. chromatographic grade. A.2.1.5 Ammonia water. A.2.1.6 1mol/L sodium hydroxide solution. Weigh 40.0g of sodium hydroxide, dissolve it in carbon dioxide-free water, and bring the volume to 1L. A.2.1.7 Pyridine sodium hydroxide solution. Pipette 100 mL of pyridine, add 30 mL of 1 mol/L sodium hydroxide solution, and dilute with water to the specified concentration. 300mL. A.2.2 Instruments and Equipment A.2.2.1 Analytical balance. sensitivity 0.0001g. A.2.2.2 Ultraviolet spectrophotometer. A.2.3 Identification Method A.2.3.1 Color Development Test A.2.3.1.1 Weigh 0.01 g (accurate to 0.0001 g) of heme iron sample, dissolve it in 50 mL of pyridine sodium hydroxide solution, and take... Add 15 mg of sodium hyposulfite to 5 mL of solution; the solution should turn red. A.2.3.1.2 Weigh 0.01 g (accurate to 0.0001 g) of heme iron sample into a 100 mL beaker, add 5 mL of nitric acid, heat, and dissolve. The liquid should be yellow. After cooling, it should be alkalized with ammonia water, and the solution should turn orange-yellow. A.2.3.2 Ultraviolet Spectroscopy Weigh 0.01 g (accurate to 0.0001 g) of heme iron sample, dissolve it in 50 mL of pyridine sodium hydroxide solution, and scan under ultraviolet light. The spectrum shows maximum absorption at a wavelength of 399 nm. A.3 Determination of Heme Iron (C34H32FeN4O4) Content A.3.1 Method Summary Heme iron in the sample was dissolved in pyridine sodium hydroxide, separated by liquid chromatography, and detected by ultraviolet or diode array detector. Qualitative analysis using time, quantitative analysis using the external standard method based on peak area. A.3.2 Reagents and Materials Unless otherwise specified, all reagents used are of analytical grade, and the water is Grade I water as specified in GB/T 6682. A.3.2.1 Methanol. chromatographic grade. A.3.2.2 Formic acid. chromatographic grade. A.3.2.3 Sodium hydroxide. A.3.2.4 Pyridine. A.3.2.5 1mol/L sodium hydroxide solution. Weigh 40.0g of sodium hydroxide, dissolve it in carbon dioxide-free water, and bring the volume to 1L. A.3.2.6 Pyridine sodium hydroxide solution. Pipette 100 mL of pyridine, add 30 mL of 1 mol/L sodium hydroxide solution, and dilute with water to the specified concentration. 300mL. A.3.2.7 Hydroxyheme (C34H33FeN4O5, CAS No.. 15489-90-4) Standard. Content ≥98.0%. A.3.2.8 Hydroxymethene Standard Stock Solution. Accurately weigh 0.1028 g of hydroxymethene dried at 100℃ for 3 h, and oxidize with pyridine. Dissolve and dilute to 100 mL in sodium solution (A.3.2.6). This solution is equivalent to 1 mg/mL of heme iron. Store in a brown reagent bottle at 0°C. Store at 4℃ away from light; shelf life is 7 days. A.3.2.9 Filter membrane. 0.45μm, aqueous phase. A.3.3 Instruments and Equipment A.3.3.1 High-performance liquid chromatograph. equipped with an ultraviolet detector or a diode array detector. A.3.3.2 Analytical balance. sensitivity 0.0001g. A.3.3.3 Ultrasonic generator. power greater than 180W. A.3.3.4 Centrifuge. greater than 4000 r/min. A.3.3.5 Constant temperature drying oven. A.3.4 Reference Conditions for Liquid Chromatography A.3.4.1 Chromatographic column. C18 column, 250 mm in length, 4.6 mm in inner diameter, 5 μm in particle size or equivalent column. A.3.4.2 Mobile phase. Methanol. 0.1% formic acid aqueous solution = 70. 30 (volume ratio). A.3.4.3 Flow rate. 1.0 mL/min. A.3.4.4 Column temperature. 30℃. A.3.4.5 Injection volume. 5.0 μL. A.3.4.6 Detection wavelength. 399nm. A.3.5 Analytical Steps Accurately weigh 0.1 g of the sample (accurate to 0.0001 g), dissolve it in pyridine sodium hydroxide solution (A.3.2.6), and sonicate under light-protected conditions. Dissolve for 10 minutes, cool to room temperature, transfer to a 50 mL volumetric flask, dilute to the mark with pyridine sodium hydroxide solution, mix well, and measure out the solution. 1 mL was placed in a 10 mL volumetric flask, diluted to the mark with pyridine sodium hydroxide solution, centrifuged at 4000 r/min for 10 min, and the supernatant was collected and filtered through a membrane. Filter and determine by liquid chromatography. Perform a blank test, except that the procedure is the same as for the sample test. A.3.6 Qualitative Analysis The qualitative identification method based on the consistency of retention time, according to the retention time of the hydroxymethylheme standard, has a deviation of ±2.5%. Within this range, determine the chromatographic peak of heme iron in the sample (see Appendix B). A.3.7 Quantitative Analysis Take 0.25 mL, 0.50 mL, 1.00 mL, 2.00 mL, and 3.00 mL of hydroxymethemoglobin standard stock solution (A.3.2.8) respectively. 5.00 mL was added to a 50 mL volumetric flask and diluted to volume with pyridine sodium hydroxide solution (A.3.2.6) to prepare a solution equivalent to the mass concentration of heme iron solution. Standard solutions with concentrations of 5 μg/mL, 10 μg/mL, 20 μg/mL, 40 μg/mL, 60 μg/mL, and 100 μg/mL were used for liquid chromatography determination. A standard curve was plotted with heme iron concentration on the x-axis and peak area on the y-axis. ......

Image     

Tips & Frequently Asked Questions:

Question 1: How long will the true-PDF of GB 1903.76-2025_English be delivered?

Answer: Upon your order, we will start to translate GB 1903.76-2025_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time.

Question 2: Can I share the purchased PDF of GB 1903.76-2025_English with my colleagues?

Answer: Yes. The purchased PDF of GB 1903.76-2025_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet.

Question 3: Does the price include tax/VAT?

Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countries

Question 4: Do you accept my currency other than USD?

Answer: Yes. If you need your currency to be printed on the invoice, please write an email to Sales@ChineseStandard.net. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.