GA/T 1979-2022 English PDF

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GA/T 1979-2022: Forensic sciences-Technical specifications for second generation sequencing for mitochondria) DNA
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Basic data

Standard ID: GA/T 1979-2022 (GA/T1979-2022)
Description (Translated English): Forensic sciences-Technical specifications for second generation sequencing for mitochondria) DNA
Sector / Industry: Public Security (Police) Industry Standard (Recommended)
Classification of Chinese Standard: A92
Classification of International Standard: 13.310
Word Count Estimation: 14,175
Date of Issue: 2022-03-09
Date of Implementation: 2022-08-01
Issuing agency(ies): Ministry of Public Security
Summary: This standard specifies the basic requirements for the terms and definitions, principles, testing equipment and reagents, testing procedures, and data analysis of human mitochondrial DNA next-generation sequencing testing conducted by forensic science DNA laboratories. This standard is applicable to forensic science DNA laboratories for the next-generation sequencing analysis of mitochondrial DNA for various forensic evidence materials and samples, and to provide mitochondrial DNA genetic information for forensic science practice.

GA/T 1979-2022: Forensic sciences-Technical specifications for second generation sequencing for mitochondria) DNA

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 13.310 CCSA92 People's Republic of China Public Security Industry Standards forensic science Mitochondrial DNA Next Generation Sequencing Technical Specifications Released on 2022-03-09 2022-08-01 Implementation Released by the Ministry of Public Security of the People's Republic of China

foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules for Standardization Documents" drafting. Please note that some contents of this document may refer to patents. The issuing agency of this document assumes no responsibility for identifying patents. This document was submitted by the Criminal Investigation Bureau of the Ministry of Public Security. This document is under the jurisdiction of the Forensic Examination Sub-Technical Committee (SAC/TC179/SC6) of the National Criminal Technology Standardization Technical Committee. This document was drafted by. Public Security Department of Liaoning Province, Dalian Public Security Bureau, Physical Evidence Appraisal Center of the Ministry of Public Security, Yantai Public Security Bureau, Guangzhou Public Security Bureau, Weihai Public Security Bureau. The main drafters of this document. Liu Feng, Li Mingguang, Zheng Zhejia, Ji Anan, Wang Le, Yu Jiao, Liu Li, Li Yue, Han Ying, Wang Weihua. Forensic Science Mitochondrial DNA Next Generation Sequencing Specifications

1 Scope

This document specifies the terms and definitions, principles, and inspection methods for the next-generation sequencing of human mitochondrial DNA in forensic science DNA laboratories. The basic requirements that should be complied with for test equipment and reagents, test procedures, data analysis, etc. This document is suitable for forensic science DNA laboratories to conduct mitochondrial DNA next-generation sequencing for various forensic evidence materials and samples Test analysis to provide mitochondrial DNA genetics information for forensic science practice.

2 Normative references

The contents of the following documents constitute the essential provisions of this document through normative references in the text. Among them, dated references For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document. GB/T 27025-2019 General requirements for the competence of testing and calibration laboratories GB/T 30989-2014 Technical Regulations for High-throughput Gene Sequencing GA/T 382-2014 Forensic Science DNA Laboratory Construction Specifications GA/T 383-2014 Forensic Science DNA Laboratory Inspection Specification GA/T 1161-2014 Content and format of forensic science DNA test identification documents GA/T 1693-2020 Forensic Science DNA Next Generation Sequencing Inspection Specification YY/T 1173-2010 Polymerase Chain Reaction Analyzer

3 Terms and Definitions

GB/T 30989-2014, GA/T 1693-2020 and the following terms and definitions apply to this document. 3.1 Mitochondrial DNA mitochondrialDNA; mtDNA DNA outside the nucleus of human cells, which exists independently in the cytoplasm, has a closed-loop double-stranded structure, with about 16569 base pairs; does not follow Mendelian The law of inheritance, manifested as maternal inheritance, the genetic information in the egg cell is passed on to the offspring; there is no cycle of mitosis and meiosis The genetic material is located in the organelle and is not affected by nuclear transfer; the copy number of mtDNA in a single cell is large and there is heterogeneity. 3.2 heteroplasmy When two or more mtDNA sequences exist in the same cell, tissue or individual, it is called heteroplasmy. Note. It can be divided into length heterogeneity and point heterogeneity, which are common in the following two situations. (1) Different mtDNA sequences are detected in the same tissue in the same individual; (2) Different mtDNA sequences were detected in different tissues of the same individual. 3.3 Hypervariable region hypervariableregion; HVR In the DNA region located in the non-coding region of mtDNA, the mutation rate can reach 6-17 times that of the single-copy nuclear genome, and there is no repair system, no Affected by selection pressure, more mutations were accumulated, and the polymorphism was good. Note. Also known as the control region (control region), or D-loop region (D-loop region), usually divided into hypervariable region I according to the position on the mtDNA chain ......

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