GB/T 42425-2023 PDF English
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Determination of capryloyl salicylic acid, phenylethyl resorcinol, ferulic acid in cosmetics - High performance liquid chromatography
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GB/T 42425-2023: PDF in English (GBT 42425-2023) GB/T 42425-2023
GB
NATIONAL STANDARD OF THE
PEOPLE'S REPUBLIC OF CHINA
ICS 71.100.70
CCS Y 42
Determination of capryloyl salicylic acid, phenylethyl
resorcinol, ferulic acid in cosmetics - High performance
liquid chromatography
ISSUED ON: MARCH 17, 2023
IMPLEMENTED ON: OCTOBER 1, 2023
Issued by: State Administration for Market Regulation;
Standardization Administration of PRC.
Table of Contents
Foreword ... 3
1 Scope ... 4
2 Normative references ... 4
3 Terms and definitions ... 4
4 Principles ... 5
5 Reagents and materials ... 5
6 Instruments and equipment ... 5
7 Test steps ... 6
8 Result calculation ... 8
9 Recovery rate ... 8
10 Precision ... 8
Appendix A (Informative) Information on capryloyl salicylic acid, phenylethyl
resorcinol, and ferulic acid standard substances ... 9
Appendix B (Informative) High-performance liquid chromatogram and DAD
spectrogram of capryloyl salicylic acid, phenylethyl resorcinol and ferulic acid ... 10
Determination of capryloyl salicylic acid, phenylethyl
resorcinol, ferulic acid in cosmetics - High performance
liquid chromatography
WARNING -- The personnel using this document shall have practical experience
in formal laboratory work. This document does not indicate all possible safety
issues. The user is responsible for taking appropriate safety and health measures
and ensuring compliance with the conditions stipulated by relevant national
regulations.
1 Scope
This document describes the high-performance liquid chromatography method for the
determination of capryloyl salicylic acid, phenylethyl resorcinol, and ferulic acid in
cosmetics, including principles, reagents and materials, instruments and equipment, test
steps, test data processing, recovery rate, precision, etc.
This document is applicable to the determination of capryloyl salicylic acid,
phenylethyl resorcinol, and ferulic acid in water, lotion, and cream cosmetics.
In this document, the method detection limit and quantification limit of capryloyl
salicylic acid, phenylethyl resorcinol, and ferulic acid are 3.3 mg/kg and 10 mg/kg,
respectively.
2 Normative references
The following documents contain the provisions which, through normative reference in
this document, constitute the essential provisions of this document. For the dated
referenced documents, only the versions with the indicated dates are applicable to this
document; for the undated referenced documents, only the latest version (including all
the amendments) is applicable to this document.
GB/T 6682 Water for analytical laboratory use - Specification and test methods
3 Terms and definitions
There are no terms and definitions that need to be defined in this document.
4 Principles
The sample is vortex-oscillated with methanol solution, treated through ultrasonic
extraction, centrifugal precipitation, separation and filtration, separated by reversed-
phase high-performance liquid chromatography, detected by a diode array detector, and
quantified by external standard method.
5 Reagents and materials
Unless otherwise specified, only analytically pure reagents are used.
5.1 Water: Grade 1 specified in GB/T 6682.
5.2 Acetonitrile (chromatographically pure).
5.3 Methanol.
5.4 Capryloyl salicylic acid, phenylethyl resorcinol, and ferulic acid: The content is not
less than 95%. See Table A.1 in Appendix A for the English name, INCI name
(International Name of Cosmetic Ingredients), CAS number, molecular formula,
relative molecular mass, and chemical structural formula.
5.5 Sea sand, chemically pure.
5.6 Sodium decanesulfonate.
5.7 Phosphoric acid.
5.8 Standard stock solution of capryloyl salicylic acid, phenylethyl resorcinol, and
ferulic acid: Accurately weigh 25.0 mg of each of capryloyl salicylic acid, phenylethyl
resorcinol, and ferulic acid (5.4) in a 25 mL volumetric flask, and dilute to the mark
with methanol (5.3). A standard stock solution with a mass concentration of 1000 μg/mL
is obtained; it is stored in a -18 °C refrigerator, and the validity period is 6 months.
5.9 Polytetrafluoroethylene (PTFE) filter membrane: The pore size is 0.22 μm.
6 Instruments and equipment
6.1 High-performance liquid chromatograph (diode array detector).
6.2 Analytical balance: The sense quantity is 0.1 mg and 0.001 g.
6.3 Volumetric flask: 10 mL, 25 mL, and 100 mL.
6.4 Graduated centrifuge test tube with a stopper: 10 mL.
6.5 Ultrasonic oscillator: The power is not less than 180 W.
6.6 Centrifuge: The rotation speed is not less than 10000 r/min.
6.7 Vortex oscillator.
6.8 Pipette or pipettor: 1 mL, 5 mL, and 10 mL.
7 Test steps
7.1 Sample treatment
For water cosmetics, take 1 g of the sample (accurate to 0.001 g) into a graduated
centrifuge tube with a stopper, add 7 mL of methanol (5.3), vortex-oscillate for 1 minute,
extract with ultrasonic for 20 minutes, and cool to room temperature; make up the
volume to 10 mL with methanol (5.3), centrifuge at 10000 r/min for 10 min, and filter
the supernatant through a 0.22 μm filter membrane (5.9) for later testing.
For facial masks (the mask liquid taken from facial masks), lotions, and cream
cosmetics, take 1 g of the sample (accurate to 0.001 g) into a graduated centrifuge tube
with a stopper, add methanol (5.3) to make up the volume to 10 mL, vortex-oscillate
for 1 minute, and conduct ultrasonic extraction for 20 min; after cooling to room
temperature, add 0.5 g~1.0 g of sea sand (5.5), vortex for 1 min, centrifuge at 10000
r/min for 10 min, and filter the supernatant through a 0.22 μm filter membrane (5.9) for
later testing.
Do two experiments in parallel.
7.2 Determination conditions
The reference conditions for high-performance liquid chromatography determination
are as follows:
a) Chromatographic column specifications: C18 chromatographic column, 250
mm×4.6 mm (particle size of 5 μm); or, a chromatographic column with
equivalent performance;
b) Mobile phase: 5 mmol/L sodium decanesulfonate solution (containing 0.1%
phosphoric acid) (A): acetonitrile (B), and the gradient elution procedure is
shown in Table 1;
c) Column temperature: 35 ℃;
d) Mobile phase flow rate: 1.0 mL/min;
......
Source: Above contents are excerpted from the PDF -- translated/reviewed by: www.chinesestandard.net / Wayne Zheng et al.
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