GB/T 36004-2018 PDF in English
GB/T 36004-2018 (GB/T36004-2018, GBT 36004-2018, GBT36004-2018)
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| GB/T 36004-2018 | English | 90 |
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Food contact surfaces cleaning and disinfection efficacy test method -- ATP bioluminescence method
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GB/T 36004-2018: PDF in English (GBT 36004-2018) GB/T 36004-2018
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 71.100.40
Y 43
Food contact surfaces cleaning and disinfection
efficacy test method - ATP bioluminescence method
ISSUED ON. MARCH 15, 2018
IMPLEMENTED ON. OCTOBER 01, 2018
Issued by. General Administration of Quality Supervision, Inspection and
Quarantine of the People's Republic of China;
Standardization Administration of the People's Republic of
China.
Table of Contents
Foreword ... 3
1 Scope ... 4
2 Normative references ... 4
3 Terms and definitions ... 4
4 Principle ... 5
5 ATP test methods ... 5
6 Laboratory cleaning and disinfection efficacy evaluation method ... 8
Food contact surfaces cleaning and disinfection
efficacy test method - ATP bioluminescence method
1 Scope
This Standard specifies a test method for the evaluation of food contact
surfaces cleaning and disinfection efficacy, based on the principle of adenosine
triphosphate (ATP) bioluminescence.
This Standard is applicable to the evaluation of food contact surfaces cleaning
and disinfection efficacy by ATP bioluminescence method.
2 Normative references
The following referenced documents are indispensable for the application of
this document. For dated references, only the edition cited applies. For undated
references, the latest edition of the referenced document (including any
amendments) applies.
GB/T 6682 Water for analytical laboratory use - Specification and test
methods
3 Terms and definitions
For the purpose of this document, the following terms and definitions apply.
3.1
food contact surfaces
Surfaces such as food packaging materials and containers, and utensils,
tableware, equipment, floors, walls in food processing, production and
management process that may be in direct contact with food.
3.2
cleaning
Process of removing the contamination from the item so as to achieve the
intended use or further processing requirement.
After starting and calibrating the instrument according to the instrument
instructions, fully PRESS the sampling rod into the test tube to activate the ATP
luminescence reaction. Quickly OSCILLATE back and forth the sampling rod
for about 5 s, MIX the reagents thoroughly, and INSERT the test tube into the
instrument for test. The measured results are expressed as relative light unit
(RLU).
5.1.5 Precautions
Note the following when using the handheld ATP bioluminescence tester smear
method.
a) Do not touch the sampling head and the smearing surface during the
smearing process;
b) The operator shall perform a consistent smearing method to obtain
repeatable results;
c) Once the sampling is completed, it is recommended to test immediately;
once activated, the test shall be carried out within 30 s;
d) The smear sampling rods provided by different manufacturers shall be
tested with their own matching instruments. The test results can refer to
the standards provided by the instrument supplier. People can be
engaged in test after being trained and deemed qualified by the supplier;
e) Different instrument test values cannot be compared together. When using
different instruments from different manufacturers, the results may be
inconsistent.
5.2 Method 2. benchtop chemiluminescence analyzer test method
5.2.1 Reagents and materials
Unless otherwise stated, only analytically pure reagents and third- or higher-
grade water as specified in GB/T 6682 are used in the analysis.
5.2.1.1 ATP test kit. The kit includes cell lysate, assay buffer, fluorescein-
luciferase mixture, ATP standard solution.
5.2.1.2 Sterile water. Experimental water after autoclaving. The sterilization
condition is. pressure 103 kPa, steam sterilization at 121 °C ~ 126 °C for 20
min. This condition is used for the sterilization operations in this Standard.
5.2.1.3 Autoclaved metal tweezer.
5.2.1.4 Filter paper. CUT the filter paper into 5 cm × 5 cm size and use it after
c) The ATP in the sample after cracking is not too stable at room temperature
so that it shall operate at 4 °C or on ice. ATP can be stabilized on ice for
up to 6 hours;
d) Bacteria can survive and multiply in sterile water, which can cause ATP
interference, so the quality of sterile water shall be checked regularly.
6 Laboratory cleaning and disinfection efficacy
evaluation method
6.1 Instruments
6.1.1 Autoclave.
6.1.2 Constant temperature incubator.
6.1.3 Centrifuge.
6.1.4 Biological safety cabinet.
6.2 Reagents and materials
6.2.1 E. coli strain.
6.2.2 LB liquid medium. Preparation method. ADD 10 g of tryptone, 5 g of yeast
extract, and 10 g of NaCl into 950 mL of experimental water, SHAKE the
container until the solute is dissolved. USE 5 mol/L NaOH to adjust the pH to
7.0, ADD experimental water to 1 L, and CARRY OUT steam sterilization at 103
kPa for 20 min.
6.2.3 Disposable plastic sterile petri dish with a diameter of 9 cm.
6.2.4 Sterilized utility knife.
6.2.5 Sterilized cotton swab.
6.2.6 Sterilized centrifuge tube.
6.3 Artificial containment
6.3.1 Bacterial culture
After activation of the E. coli strain, take 1 mL of E. coli culture medium to
inoculate into 100 mL of sterilized LB liquid medium, and culture in a 37 °C
incubator at a rate of 220 r/min for 24 h.
......
Source: Above contents are excerpted from the PDF -- translated/reviewed by: www.chinesestandard.net / Wayne Zheng et al.
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