GB/T 13917.2-2009 PDF in English
GB/T 13917.2-2009 (GB/T13917.2-2009, GBT 13917.2-2009, GBT13917.2-2009)
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Laboratory efficacy test methods and criterions of public health insecticides for pesticide registration -- Part 2: Aerosol
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Standards related to: GB/T 13917.2-2009
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GB/T 13917.2-2009: PDF in English (GBT 13917.2-2009) GB/T 13917.2-2009
GB
NATIONAL STANDARD OF THE
PEOPLE’S REPUBLIC OF CHINA
ICS 65.100
B 17
Replacing GB 13917.2-1992, GB/T 17322.2-1998
Laboratory efficacy test methods and criterions of public
health insecticides for pesticide registration - Part 2: Aerosol
ISSUED ON: MARCH 27, 2009
IMPLEMENTED ON: OCTOBER 01, 2009
Issued by: General Administration of Quality Supervision, Inspection and
Quarantine of PRC;
National Standardization Administration.
Table of Contents
Foreword ... 3
1 Scope ... 5
2 Test materials ... 5
3 Instruments and equipment ... 5
4 Test method ... 6
5 Calculation ... 7
6 Evaluation ... 8
7 Results and report preparation ... 8
Foreword
GB/T 13917 "Laboratory efficacy test methods and criterions of public health
insecticides for pesticide registration" is divided into 10 parts:
- Part 1: Spray fluid;
- Part 2: Aerosol;
- Part 3: Smoke generator and smoke tablet;
- Part 4: Mosquito coil;
- Part 5: Vaporizing mat;
- Part 6: Liquid vaporizer;
- Part 7: Bait;
- Part 8: Dutsable powder and chalk;
- Part 9: Repellent;
- Part 10: Analogous site.
This Part is Part 2 of GB/T 13917.
This Part replaces GB 13917.2-1992 "Efficacy test methods of public health
insecticides for pesticides registration. Method of laboratory efficacy test for aerosol"
and GB/T 17322.2-1998 "Laboratory efficacy criterions of public health insecticides
for pesticide registration - Aerosol".
Compared with GB 13917.2-1992 and GB/T 17322.2-1998, the main changes in this
Part are as follows:
- MERGE GB 13917.2-1992 and GB/T 17322.2-1998, so that methods and
evaluations can be reflected in the same standard, making application more
convenient;
- MODIFY the requirements for standard test insects into: use standard test insects
of sensitive strains raised in the laboratory;
- MODIFY and CLARIFY the age of the tested insects;
- MODIFY the test instruments;
- No longer required to set up a blank control test;
Laboratory efficacy test methods and criterions of public
health insecticides for pesticide registration - Part 2: Aerosol
1 Scope
This Part of GB/T 13917 specifies the indoor efficacy measurement methods and
evaluation criterions for aerosols.
This Part is applicable to the determination and evaluation of the efficacy of aerosols,
which are directly sprayed on health pests mosquitoes, flies, cockroaches, during
pesticide registration.
2 Test materials
Use standard test insects of sensitive strains raised in the laboratory.
2.1 Mosquitoes
Culex pipiens pallens (northern region) or Culex pipiens quinque fasciatus (southern
region), which are female adults that do not feed on blood on the 3rd to 5th day after
emergence.
2.2 Flies
Musca elegans, which is an adult on the 3rd to 4th day after emergence, half male and
half female.
2.3 Cockroach
Blattella germanica, which is a 10th to 15th day adult, half male and half female.
3 Instruments and equipment
3.1 Cylinder device (Figure 1)
The colorless transparent cylinder (C) is placed on the bracket (I). A pull plate (A) is
inserted into the upper frame of the bracket. There is a colorless transparent cylinder
(or tube) (B) under the pull plate. There is an insect hole (J) in the middle of the side
wall. The hole is plugged with a rubber plug, after putting the test insect in. There is a
12-mesh screen cover (H) on the mouth of the colorless transparent cylinder or tube (B).
There are pillars (F) on the bottom of the bracket (I), to make the cylinder (or tube) (B)
Relative humidity: 60% ± 10%.
4.2 Test steps
4.2.1 Mosquitoes and flies
Use a cylinder device (3.1). Release the test insects (30 houseflies or 30 mosquitoes)
into B, through the insect hole (J). After the test insects resume normal activities, place
the aerosol tube to be tested in a horizontal shape, with the nozzle pointing downwards
and vertically. Aim at the spray hole on E, to spray the agent (1.0 ± 0.1) g. Immediately
plug the round hole with a rubber stopper (G). After 1 minute, pull out the pull plate
(A). Start timekeeping immediately. Record the number of knocked down test insects
at regular intervals. After 20 min, move the knocked down test insects into clean insect
cages. Resume standard feeding. Feed with 5% sugar water cotton balls. Check the
number of dead insects for 24 hours. The test insects that are not knocked down are
counted as live insects. The test shall be repeated three or more times. After each test,
the test device shall be cleaned.
4.2.2 Cockroach
Use the cylinder device (3.1) instead of the pull plate (A). Place 20 cockroaches in a
cylinder B, which is coated with a circle of Vaseline on the upper part of the inner wall;
sealed with a 12-mesh iron screen. After the test insects resume normal activities, place
the aerosol cylinder horizontally, with the nozzle directed downwards and vertically.
Spray the pesticide (1.0 ± 0.1) g into the spray hole on E. Immediately plug the round
hole with a rubber stopper (G). Start timekeeping. Record the number of knocked down
test insects at certain intervals. After 20 minutes, move all the test insects to a clean
vessel. Resume standard feeding. It should feed them with mixed feed blocks and water-
soaked cotton balls. Check the number of dead insects after 72 hours. The test shall be
repeated three or more times. After each test, the test device shall be cleaned.
5 Calculation
The repeated test data are used to calculate KT50 and virulence regression equations,
according to the linear weighted regression method. The 24 h (72 h for cockroaches)
mortality is calculated according to formula (1); the results are retained to 2 decimal
places.
Where:
P - Mortality rate, %;
H - Represents the number of dead insects, in pcs;
......
Source: Above contents are excerpted from the PDF -- translated/reviewed by: www.chinesestandard.net / Wayne Zheng et al.
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