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GB 4789.30-2010 (GB 4789.30-2016 Newer Version) PDF English


Search result: GB 4789.30-2010 (GB 4789.30-2016 Newer Version)
Standard IDContents [version]USDSTEP2[PDF] delivered inName of Chinese StandardStatus
GB 4789.30-2016English115 Add to Cart 0-9 seconds. Auto-delivery. National Food Safety Standard -- Food Microbiological Examination -- Examination of Listeria Monocytogenes Valid
GB 4789.30-2010English70 Add to Cart 0-9 seconds. Auto-delivery. National food safety standard -- Food microbiological examination: Listeria monocytogenes Obsolete
GB/T 4789.30-2008English679 Add to Cart 5 days Microbiological examination of food hygiene -- Examination of listeria monocytogenes Obsolete
GB/T 4789.30-2003English359 Add to Cart 3 days Microbiological examination of food hygiene -- Examination of listeria monocytogenes Obsolete
GB 4789.30-1994EnglishRFQ ASK 3 days Microbiological examination of food hygiene. Examination of Listeria moncytogenes Obsolete
BUY with any currencies (Euro, JPY, GBP, KRW etc.): GB 4789.30-2016     Newer version: GB 4789.30-2016

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GB 4789.30-2010: PDF in English

GB 4789.30-2010 GB National Standard of the People’s Republic of China National food safety standard Food microbiological examination. Listeria monocytogenes ISSUED ON. MARCH 26, 2010 IMPLEMENTED ON. JUNE 01, 2010 Issued by. Ministry of Health of the People’s Republic of China Table of Contents Foreword ... 3  1 Scope ... 4  2 Apparatuses and materials ... 4  3 Culture medium and reagents ... 5  4 Inspection procedure ... 5  5 Operation steps ... 6  6 Results and report ... 8  Appendix A ... 9  Foreword  This standard will replace GB/T 4789.30-2008 Microbiological examination of food hygiene - Examination of listeria monocytogenes. Compared with GB/T 4789.30-2008, the main changes of this standard are as follows. - Modify this standard’s Chinese and English names; - Delete “the second method - Automatic enzyme-linked fluorescence immunoassay analyzer screening method”; - Delete “the third method - Automatic screening method of pathogen detection system”. This standard’s appendix A is normative. The previous versions replaced by this standard are. - GB 4789.30-1994, GB/T 4789.30-2003, GB/T 4789.30-2008. National food safety standard Food microbiological examination. Listeria monocytogenes 1 Scope This standard specifies the test methods of Listeria monocytogenes in foods. This standard applies to test of Listeria monocytogenes in foods. 2 Apparatuses and materials    In addition to the conventional sterilization and cultivation apparatuses, other apparatuses and materials are as follows. 2.1 Refrigerator. 2°C~5°C. 2.2 Thermostatic incubator. 30°C±1°C, 36°C±1°C. 2.3 Homogenizer. 2.4 Microscope. 10x~100x. 2.5 Electronic balance. Sensitivity is 0.1g. 2.6 Erlenmeyer flasks. Capacity 100mL and 500mL. 2.7 Sterile pipette. 1mL (with 0.01mL scale), 10mL (with 0.1mL scale). 2.8 Sterile petri dish. Diameter is 90mm. 2.9 Sterile test tube. 16mmx160mm. 2.10 Centrifuge tube. 30mmx100mm. 2.11 Sterile syringe. 1mL. 2.12 Staphylococcus aureus (ATCC25923). 2.13 Rhodococcus equi. 2.14 Mus musculus albus. 16g - 18g. 2.15 Automated microbial and biochemical identification system. 3 Culture medium and reagents    3.1 Tryptone soya broth containing 0.6% yeast extract (TSB-YE). see appendix A.1. 3.2 Trypticase soy agar containing 0.6% yeast extract (TSA-YE). see appendix A.2. 3.3 Listeria enrichment broth LB (LB1, LB2). see appendix A.3. 3.4 1% acriflavine HCL solution. see appendix A.3.2.1. 3.5 1% of naladixic acid solution. see appendix A.3.2.1. 3.6 PALCAM agar. see appendix A.4. 3.7 Gram dye. see appendix A.5. 3.8 SIM motility culture medium. see appendix A.6. 3.9 Buffer glucose peptone water [for Methyl Red (MR) and V-P tests]. see appendix A.7. 3.10 5%~8% sheep blood agar. see appendix A.8. 3.11 Sugar fermentation tube. see appendix A.9. 3.12 Catalase test. see appendix A.10. 3.13 Listeria chromogenic medium. 3.14 Biochemical identification kit. 4 Inspection procedure  The inspection procedure of Listeria monocytogenes is shown in Figure 1. A.9.2 Preparation method A.9.2.1 Allocate glucose fermentation tubes according to above compositions. Add glucose according to proportion 0.5%. Pack into the small test tubes which have inverted tubules. Adjust pH to 7.4. Autoclave it for 15min at 115°C. Preserve for spare use. A.9.2.2 Allocate other glucose fermentation tubes according to above compositions. Pack every bottle for 100mL. Autoclave it for 15min at 115°C. Allocate all types of carbohydrate to make 10% solution. Autoclave it simultaneously. Add 5mL of sugar solution to 100mL of culture medium. Use sterile technique to pack into small tubes. A.9.3 Test method Use appropriate pure cultures to inoculate in sugar fermentation tube. Cultivate it for 24h~48h at 36°C±1°C. Observe the result. Blue represents negative. Yellow represents positive. A.10 Catalase test A.10.1 Reagents 3% Hydrogen peroxide solution. Prepare it when use. A.10.2 Test method Use small glass rod or disposable inoculation needle to pick single colony. Place it in clean test tube. Add 2mL of 3% Hydrogen peroxide solution. Observe the result. A.10.3 Results If it produces bubbles within half minute, the result is positive. If not, the result is negative. ......
Source: Above contents are excerpted from the PDF -- translated/reviewed by: www.chinesestandard.net / Wayne Zheng et al.