GB/T 42699.2-2023 English PDF

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GB/T 42699.2-2023: Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 2: Peptide detection using MALDI-TOF-MS
Status: Valid

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Similar standards

GB/T 42701   GB/T 42697   GB/T 42698   GB/T 42694   GB/T 42695   GB/T 42699.1   

Basic data

Standard ID: GB/T 42699.2-2023 (GB/T42699.2-2023)
Description (Translated English): Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 2: Peptide detection using MALDI-TOF-MS
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: W04
Classification of International Standard: 59.080.01
Word Count Estimation: 22,283
Date of Issue: 2023-08-06
Date of Implementation: 2024-03-01
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 42699.2-2023: Textiles - Qualitative and quantitative proteomic analysis of some animal hair fibres - Part 2: Peptide detection using MALDI-TOF-MS

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
ICS 59.080.01 CCS W04 National Standards of People's Republic of China Textile proteome characterization of certain animal hair fibers and Quantitative Analysis Part 2.Reduced Protein Peptides Analytical matrix-assisted laser desorption ionization Time-of-flight mass spectrometry (MALDI-TOF-MS) method (ISO 20418-2.2018,MOD) Published on 2023-08-06 2024-03-01 Implementation State Administration for Market Regulation Released by the National Standardization Administration Committee

Table of contents

PrefaceⅠ Introduction II 1 Scope 1 2 Normative reference documents 1 3 Terms and Definitions 1 4 Abbreviations 2 5 Principle 2 6 reagent 2 7 Instruments and Utensils 4 8 Test step 4 9 Precision 7 10 Interlaboratory Test 7 11 Test report 7 Appendix A (informative) Example 8 of SDS-PAGE gel image of extracted protein Appendix B (Informative) Animal species-specific mass spectrum peak 9 analyzed by MALDI-TOF-MS Appendix C (Informative) Species-specific monoisotopic peaks in MALDI-TOF-MS analysis10 Appendix D (informative) Standard curve for cashmere-sheep wool mixture 11 Appendix E (informative) Standard curve of cashmere-yak blend 12 Appendix F (Informative) Repeatability and Reproducibility 13 Appendix G (informative) MALDI-TOF-MS blind sample detection and analysis 15 Appendix H (Informative) Interlaboratory Validation 16 Reference 19

Foreword

This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. This document is Part 2 of GB/T 42699 "Qualitative and Quantitative Analysis of Proteome of Certain Animal Hair Fibers in Textiles". GB/T 42699 has released the following parts. ---Part 1.Reduced protein peptide analysis liquid chromatography-mass spectrometry (LC-ESI-MS) method; ---Part 2.Reduced protein peptide analysis matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) method. This document is modified using ISO 20418-2.2018 "Qualitative and quantitative analysis of proteome of certain animal hair fibers in textiles - Part 2" Points. Reduced protein peptide analysis matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) method. The technical differences between this document and ISO 20418-2.2018 and their reasons are as follows. ---Added warning; ---In Chapter 1, ISO 1833 (all parts) is replaced by our country's document GB/T 2910 (all parts), FZ/T 01057.3 generation Replaced ISO /T R11827, GB/T 40905 (all parts) replaced ISO 17551 (all parts) to adapt to our country's technology technical conditions; ---In Chapter 6, the regulations on reagents are made in line with the actual situation in our country; ---In 6.1, ISO 3696 is replaced by my country's document GB/T 6682 to adapt to my country's technical conditions; ---In 6.4, the mass of sodium dihydrogen phosphate is corrected to 24g; ---In 6.14, the CAS number of the substance is added; ---In Chapter 7, 7.9 "centrifuge tube", 7.10 "96-well microplate", 7.11 "pH meter" and 7.12 "centrifuge" are added; ---In 8.3.4, the recycling strip size is increased to make it more operable; ---In 8.5.1, change the reflection mode to reflection mode or linear mode. The following editorial changes have been made to this document. ---In 3.1, the definition of animal hair fiber is adjusted to be consistent with the definition in ISO 20418-1.2018. Please note that some content in this document may be subject to patents. The publisher of this document assumes no responsibility for identifying patents. This document is proposed by the China Textile and Apparel Industry Federation. This document is under the jurisdiction of the National Textile Standardization Technical Committee (SAC/TC209). This document was drafted by. Shanghai Customs Industrial Products and Raw Materials Testing Technology Center, China Textile Standard Inspection and Certification Co., Ltd., Anhui Zheng Lin Textile Co., Ltd., Wujiang Meijin Weaving Co., Ltd., Zhejiang Yilei Wool Textile Co., Ltd., Changxing Kingfa Textile Co., Ltd., Jinjiang Guangzhilin Knitting Co., Ltd., Shanghai Aili Textile Technology Inspection Co., Ltd., Dongguan Weiside Technology Development Co., Ltd., Hunan Keli Jia Textile Co., Ltd., Yiwu Niqing Household Products Co., Ltd. The main drafters of this document. Fei Jing, Chen Xiao, Fei Yafei, Wei Mengyuan, Si Ying, Gao Linmei, Yao Ling, Wang Ya, Zhang Chunhua, Shen Yilei, Jin Fei, Zhang Wenfu, Xu Shanwen, Zhang Zhihuan.

Introduction

For the qualitative and quantitative analysis test methods of certain animal hair fiber proteomes in textiles, for the convenience of use, according to different test principles The principles and methods are divided into multiple parts. GB/T 42699 "Qualitative and Quantitative Analysis of Proteome of Certain Animal Hair Fibers in Textiles" is planned to be composed of the following two parts. Composed of parts. ---Part 1.Reduced protein peptide analysis liquid chromatography-mass spectrometry (LC-ESI-MS) method. The purpose is to establish the use of liquid phase color Spectrometry-mass spectrometry (LC-ESI-MS) is a qualitative and quantitative method for determining the components of animal hair fiber mixtures in textiles. ---Part 2.Reduced protein peptide analysis matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) method. The purpose is to establish the use of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to determine the dynamic properties of textiles. Qualitative and quantitative methods for the components of wool fiber mixtures. Textile proteome characterization of certain animal hair fibers and Quantitative Analysis Part 2.Reduced Protein Peptides Analytical matrix-assisted laser desorption ionization Time-of-flight mass spectrometry (MALDI-TOF-MS) method Warning---Personnel using this document should have practical experience in regular laboratory work. This document does not address all possible security issues question. Users are responsible for taking appropriate safety and health measures and ensuring compliance with the conditions stipulated in relevant national regulations.

1 Scope

This document specifies the use of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) for the determination of animal hair in textiles. Qualitative and quantitative methods for fiber mixture components. Non-animal hair fiber components can be tested with reference to GB/T 2910 (all parts), and then the results are combined to determine the overall fiber content. This method refers to FZ/T 01057.3 and uses an optical microscope to observe the appearance of mixed fibers for preliminary identification. This method is not suitable Used when animal fibers of the same species are present (e.g. mixtures of cashmere and mohair), such samples can be analyzed by microscopic examination Quantitative (such as the analytical methods described in GB/T 40905.1 and GB/T 40905.2).

2 Normative reference documents

The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, the dated quotations For undated referenced documents, only the version corresponding to that date applies to this document; for undated referenced documents, the latest version (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods (GB/T 6682-2008, ISO 3696.1987, MOD)

3 Terms and definitions

The following terms and definitions apply to this document. 3.1 animal hair fiber animalhairfibre Animal hair follicles grow from fibers with a multicellular structure composed of keratin. Examples. cashmere, sheep wool, yak wool, etc. 3.2 protein protein Amino acid polymers that play important roles in living things. 3.3 polypeptide A small fragment of protein (3.2) formed by dehydration and condensation of less than 50 amino acids. 3.4 buffer solution A solution that maintains the pH of the reaction solution within an appropriate range. ......

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