GB/T 40254-2021 English PDF

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GB/T 40254-2021: Detection and identification of Verticillium Nees using real-time PCR
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GB/T 40254-2021	189	Add to Cart	3 days	Detection and identification of Verticillium Nees using real-time PCR	Valid

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Basic data

Standard ID: GB/T 40254-2021 (GB/T40254-2021)
Description (Translated English): Detection and identification of Verticillium Nees using real-time PCR
Sector / Industry: National Standard (Recommended)
Classification of Chinese Standard: B16
Word Count Estimation: 10,169
Issuing agency(ies): State Administration for Market Regulation, China National Standardization Administration

GB/T 40254-2021: Detection and identification of Verticillium Nees using real-time PCR

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Verticillium Nees using real-time PCR ICS 65.020.01 CCSB16 National Standards of People's Republic of China Verticillium spp. real-time fluorescent PCR quarantine and identification method Released on 2021-05-21 2021-12-01 implementation State Administration of Market Supervision and Administration Issued by the National Standardization Management Committee

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1.Structure and Drafting Rules of Standardization Documents" Drafting. Please note that some of the contents of this document may involve patents. The issuing agency of this document is not responsible for identifying patents. This document was proposed and managed by the National Plant Quarantine Standardization Technical Committee (SAC/TC271). Drafting organizations of this document. Ningbo Customs of the People's Republic of China, Institute of Microbiology, Chinese Academy of Sciences, Xining Vegetable Technical Service Center, Chinese Academy of Inspection and Quarantine Sciences, Urumqi Customs of the People's Republic of China. The main drafters of this document. Duan Weijun, Cai Lei, Zhang Huili, Zhao Peng, Zhang Xiaomei, Guo Lixin, Liu Fang, Li Xuelian, Zhang Yongjiang, Zhang Xiaoju, Chen Xianfeng. Verticillium spp. real-time fluorescent PCR quarantine and identification method

1 Scope

This document describes the real-time fluorescent polymerase chain reaction (PCR) quarantine identification method for the plant pathogenic fungus Verticillium. This document is applicable to the detection and screening of Verticillium pathogens in soil, plants and their products that carry Verticillium pathogens.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to This document. SN/T 2589 Test specification for plant pathogenic fungi

3 Terms and definitions

There are no terms and definitions that need to be defined in this document.

4 Basic information

Chinese name. Verticillium. Scientific name. Verticillium Nees. Verticillium belongs to Fungi, Ascomycota, Pezizomycotina, Faecomycetes (Sordariomycetes) in the small irregular spherical shell family (Plectosphaerelaceae). Transmission route. Verticillium pathogens are mainly spread by seeds, but also by diseased bodies, soil, wind, irrigation water and insects.

5 Principles of Identification

According to the DNA sequence characteristics of Verticillium pathogens and the principle of real-time fluorescent PCR, the designed primers or probes are used to treat Verticillium diseases Bacteria are tested and identified. 6 Equipment and utensils, reagents and culture medium 6.1 Instruments and appliances Autoclave, microscope, dissecting mirror, balance, water bath, ice maker, pure water meter, vortex oscillator, trace ultraviolet spectrophotometer, high Quick-freezing centrifuge, refrigerator, ultra-clean workbench, biochemical incubator, fume hood, real-time fluorescent PCR instrument, micropipette (0.1μL~2.5μL, 1μL~10μL, 10μL~50μL, 20μL~200μL, 100μL~1000μL), Erlenmeyer flask, Petri dish, glass slide, cover slip. ......

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